Difference between revisions of "LuxR/cI"

Revision as of 15:58, 2 July 2008

Parallel Biobrick Assembly

Objective 1: pLux/cI + RBS + LuxS + RBS + Mnt + TT (ampicillin resistance)

K091107 + B0034 + K091109 + B0034 + C0072 + B0015[B0010+B0012]

Step	BBa_	Description	Prefix/suffix	Enzymes	Size	BBa_	Description	Prefix/suffix	Enzymes	Size	BBa_	Size
.	Vector	.	.	.	.	Insert	.	.	.	.	Product	.
1A	B0015	Double Terminator	RX/SP	RX	129bp	C0072	Mnt Gene	RX/SP	RS	288bp
1B	B0034	RBS	RX/SP	RX	12bp	K091109	LuxS Gene	RX/SP	RS	516bp
2	B0034	RBS	RX/SP	SP	12bp	Product of 1B	LuxS + RBS	RX/SP	XP
3	Product of 2	RBS + LuxS + RBS	RX/SP	SP		Product of 1A	Mnt Gene + TT	RX/SP	XP
4	K091107	pLux/cI	RX/SP	SP		Product of 3	RBS + LuxS + RBS + Mnt + TT	RX/SP	XP			Objective 1

Objective 2: pBAD + RBS + LuxR + TT (kanamycin resistance)

@@ Line 30: / Line 30: @@
 | 3 || Product of 2 || RBS + LuxS + RBS || RX/SP || SP ||  || Product of 1A || Mnt Gene + TT || RX/SP || XP || || ||
 |-
-| 4 || K091107 || pLux/cI || RX/SP || SP ||  || Product of 3 || RBS + LuxS + RBS + Mnt + TT || RX/SP || XP ||  || ||
+| 4 || K091107 || pLux/cI || RX/SP || SP ||  || Product of 3 || RBS + LuxS + RBS + Mnt + TT || RX/SP || XP ||  || || Objective 1
-|-
-| || ||  ||  ||  || || || || || || || ||
-|-
-| || ||  ||  ||  || || || || || || || ||
-|-
-| || ||  ||  ||  || || || || || || || ||
 |-
 |}