Difference between revisions of "LuxR/cI"

Revision as of 16:16, 2 July 2008

Parallel Biobrick Assembly

Objective 1: pLux/cI + RBS + LuxS + RBS + Mnt + TT (ampicillin resistance)

K091107 + B0034 + K091109 + B0034 + C0072 + B0015[B0010+B0012]

Step	BBa_	Description	Prefix/suffix	Enzymes	Size	BBa_	Description	Prefix/suffix	Enzymes	Size	BBa_	Size
.	Vector	.	.	.	.	Insert	.	.	.	.	Product	.
1A	B0015	Double Terminator	RX/SP	RX	129bp	C0072	Mnt Gene	RX/SP	RS	288bp
1B	B0034	RBS	RX/SP	RX	12bp	K091109	LuxS Gene	RX/SP	RS	516bp
2	B0034	RBS	RX/SP	SP	12bp	Product of 1B	LuxS + RBS	RX/SP	XP
3	Product of 2	RBS + LuxS + RBS	RX/SP	SP		Product of 1A	Mnt Gene + TT	RX/SP	XP
4	K091107	pLux/cI	RX/SP	SP		Product of 3	RBS + LuxS + RBS + Mnt + TT	RX/SP	XP		Objective 1

Parts

Objective 2: pBAD + RBS + LuxR + TT (kanamycin resistance)

BBa_	Description	Source	location('07)	Vector
B0015	double terminator	registry 07	Plt.1-1I;Plt.3-3O	pSB1AK3
B0034	RBS	registry 07	Plt.1-3O	pSB1A2
C0072	Mnt Gene	registry 07	Plt.2-5M	pSB2K3
K091107	pLux/cI	N/A
K091109	LuxS	N/A

@@ Line 43: / Line 43: @@
 !width="35"|Vector
 |-
-|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 ||
+|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3
 |-
-|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 ||
+|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2
 |-
-|[http://partsregistry.org/Part:BBa_C0072 C0072] ||Mnt Gene ||registry 07 ||Plt.2-5M ||pSB2K3 ||
+|[http://partsregistry.org/Part:BBa_C0072 C0072] ||Mnt Gene ||registry 07 ||Plt.2-5M ||pSB2K3
 |-
-|[http://partsregistry.org/Part:BBa_K091107 K091107] ||pLux/cI || N/A || || ||
+|[http://partsregistry.org/Part:BBa_K091107 K091107] ||pLux/cI || N/A || ||
 |-
-|[http://partsregistry.org/Part:BBa_K091109 K091109] ||LuxS || N/A || || ||
+|[http://partsregistry.org/Part:BBa_K091109 K091109] ||LuxS || N/A || ||
 |-
--}