LuxR/cI

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Parallel Biobrick Assembly

Objective 1: pLux/cI + RBS + LuxS + RBS + Mnt + TT (ampicillin resistance)

K091107 + B0034 + K091109 + B0034 + C0072 + B0015[B0010+B0012]

Step BBa_ Description Prefix/suffix Enzymes Size BBa_ Description Prefix/suffix Enzymes Size BBa_ Size
. Vector . . . . Insert . . . . Product .
1A B0015 Double Terminator RX/SP RX 129bp C0072 Mnt Gene RX/SP RS 288bp
1B B0034 RBS RX/SP RX 12bp K091109 LuxS Gene RX/SP RS 516bp


Objective 2: pBAD + RBS + LuxR + TT (kanamycin resistance)


Step BBa_ Description Prefix/suffix Enzymes Size BBa_ Description Prefix/suffix Enzymes Size BBa_ Size
. Vector . . . . Insert . . . . Product .
1