LuxR/cI
From GcatWiki
Parallel Biobrick Assembly
Objective 1: pLux/cI + RBS + LuxS + RBS + Mnt + TT (ampicillin resistance)
K091107 + B0034 + K091109 + B0034 + C0072 + B0015[B0010+B0012]
| Step | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Size |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Vector | . | . | . | . | Insert | . | . | . | . | Product | . |
| 1A | B0015 | Double Terminator | RX/SP | RX | 129bp | C0072 | Mnt Gene | RX/SP | RS | 288bp | ||
| 1B | B0034 | RBS | RX/SP | RX | 12bp | K091109 | LuxS Gene | RX/SP | RS | 516bp | ||
| 2 | B0034 | RBS | RX/SP | SP | 12bp | Product of 1B | LuxS + RBS | RX/SP | XP | |||
| 3 | Product of 2 | RBS + LuxS + RBS | RX/SP | SP | Product of 1A | Mnt Gene + TT | RX/SP | XP | ||||
| 4 | K091107 | pLux/cI | RX/SP | SP | Product of 3 | RBS + LuxS + RBS + Mnt + TT | RX/SP | XP | ||||
Objective 2: pBAD + RBS + LuxR + TT (kanamycin resistance)
| Step | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Description | Prefix/suffix | Enzymes | Size | BBa_ | Size |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Vector | . | . | . | . | Insert | . | . | . | . | Product | . |
| 1 | ||||||||||||
