Difference between revisions of "Malcolm C"

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(→‎EST Search Results)
(→‎EST Search Results)
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9) MybA1
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9) MybA1<br>
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did tBLASTn using amino acid results from scaffold03500
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did tBLASTn using amino acid results from scaffold03500<br>
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LGVRKGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG
+
LGVRKGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG<br>
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−
 
 
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>DR067494        Length = 369
 
−
 
 
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Score = 43.5 bits (101), Expect = 2e-06
 
−
Identities = 18/35 (51%), Positives = 24/35 (68%)
 
−
Frame = +3
 
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−
Query: 2  GVRKGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG 36
 
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          G+ +GAWT  ED +L + I  +GEGKW  +P RAG
 
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Sbjct: 144 GLNRGAWTAMEDKILTEYIRVNGEGKWRNLPKRAG 248
 
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>CV091396        Length = 791
 
−
 
 
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Score = 41.6 bits (96), Expect = 8e-06
 
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Identities = 16/34 (47%), Positives = 22/34 (64%)
 
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Frame = +1
 
−
 
 
−
Query: 3  VRKGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG 36
 
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          ++KG W  EED +LK  +E HGEG W  V  ++G
 
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Sbjct: 19 LKKGLWKPEEDRILKNYVETHGEGNWASVSQKSG 120
 
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>CV191530        Length = 673
 
−
 
 
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Score = 39.3 bits (90), Expect = 4e-05
 
−
Identities = 16/32 (50%), Positives = 21/32 (65%)
 
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Frame = +1
 
−
 
 
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Query: 5  KGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG 36
 
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          KGAW+++ED  L  I +HGEG W  +P  AG
 
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Sbjct: 148 KGAWSKQEDQKLIDYIRRHGEGCWRTIPQAAG 243
 
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−
 
 
−
 
 
−
>CV090776        Length = 717
 
−
 
 
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Score = 38.1 bits (87), Expect = 9e-05
 
−
Identities = 17/34 (50%), Positives = 23/34 (67%), Gaps = 1/34 (2%)
 
−
Frame = +1
 
−
 
 
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Query: 3  VRKGAWTEEEDCLLKKCIEKHGE-GKWHQVPYRA 35
 
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          V+KG W+ EED  LK+ I+KHG  G W  +P +A
 
−
Sbjct: 103 VKKGPWSPEEDAKLKEYIDKHGTGGNWIALPQKA 204
 
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 +
[[File:Myba1_EST.png]]
  
 
10) MYCA1
 
10) MYCA1

Revision as of 20:09, 12 April 2012

This is where I will capture my progress on this project.

I will also be working on the 13 genes Allan gave us.


SSR Primer Hunt

The class has already completed 4 SSR primer designs (LDOX, CHS, StSy, and F3H).

Results from Blueberry 454 Scaffold Searches from Grape (given to us by Allan Brown)
AOMT Scaffold 00796

  1. Forward Primer GAGCTCTTGGATGCTCCATACT & Rev Primer CCTCATAATTTTTGGTCTTCGC (taa) x6 PCR product = 137 bp
  2. Forward Primer TATCCCATGTTTTGGCTAATCC & Rev Primer TTTGCTTTTCAATCGGGAAC (tc) x9 PCR product = 261 bp


AOMT scaffold 01781

  1. Forward Primer GGCGACTGTGTCTTTCAGTAAA & Rev Primer TAGGATTTCGAGGAGGAGAGGT (ct) x7 PCR product = 136 bp
  2. Forward Primer ATAAACAGGTAAACCGAACCGA & Rev Primer CCGATTAAATTGGAGCAGTAGC (tg) x7 PCR product = 262 bp


ANPER scaffold 00666

  1. Forward Primer TTTCTATCTCTCTCGCCGTCAT & Rev Primer GTTTTCTCACCTTGTTTTTGCC (ct) x21 PCR product = 219 bp
  2. Forward Primer CCGTTTCGATCTCTCTCTCAGT & Rev Primer CATCCCTAGATCTGACACCACA (tc) x14 PCR product = 278 bp


CHI scaffold 03186

  1. Forward Primer TACATCTTGAGGGGCAGTTTTT & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp
  2. Forward Primer ACGTGGTCCAATCTAACCTCAC & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp


DFR scaffold 03132

  1. Forward Primer TACATCTTGAGGGGCAGTTTTT & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp
  2. Forward Primer ACGTGGTCCAATCTAACCTCAC & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp


F3'H scaffold 01438

  1. Forward Primer CAGTGGTACAGTGGTGGATGAC & Rev Primer ATTCCCTTTCTCTTCCCATTTC (ag) x7 PCR product = 152 bp
  2. Forward Primer ATACCAATCCATAGTCTCCCGA & Rev Primer TCAGTTGTGTTTTTCAGTTGGG (ta) x7 PCR product = 279 bp


FLS scaffold 00142

  1. Forward Primer CAATTATACCCTTCCTGTCCCA & Rev Primer GAATGGATGAAAGGAACCCATA (ag) x8 PCR product = 214 bp
  2. Forward Primer CTAGCTCTCCCTCTCTCCCTCT & Rev Primer GTGGTACTCCATATCCATGCCT (ct) x8 PCR product = 295 bp


FLS scaffold 00374

  1. Forward Primer TGAGCATCATTGCCAGAGTAGT & Rev Primer GGATTTCATTCCAAGAGATTGC (ct) x6 PCR product = 295 bp
  2. Forward Primer TAGATTATCCGGGGAATCATTG & Rev Primer GATTCTCATGACAAGCCAGTTG (tc) x6 PCR product = 275 bp


MYBA1 scaffold 03500

  1. Forward Primer GCGAGGATGGAAATATATACGG & Rev Primer GCTTCATAAGCTGCTAGGCAAT (ag) x8 PCR product = 222 bp
  2. Forward Primer GCGAGGATGGAAATATATACGG & Rev Primer GCTTCATAAGCTGCTAGGCAAT (ttat) x5 PCR product = 222 bp


MYCA1 scaffold 00278

  1. Forward Primer CAGTATTTGAGCCGGAACCTAC & Rev Primer CGTACAGAGTTTCGCACAAAAG (ct) x7 PCR product = 268 bp
  2. Forward Primer TAGTGGGAACGAGTCGTCTTTT & Rev Primer AGTTGGTGCCCTTTTATGAAGA (at) x6 PCR product = 195 bp


MYCA1 scaffold 00554

  1. Forward Primer GGCTAAAACCCTAGTTGCCTTT & Rev Primer GGGTGTACTGAGAACCGAAGTC (tc) x6 PCR product = 157 bp
  2. Forward Primer CCCAAAAGAAAATACCTGGACA & Rev Primer GGAGCTTGGACCTAAAAGGATT (ga) x8 PCR product = 260 bp


UFGT scaffold 00304

  1. Forward Primer TGAACTACCAAAATGGGACAGA & Rev Primer NGACGGAGCACATCCTACTACA (ag) x10 PCR product = 207 bp
  2. Forward Primer CCCCAAATTCAGATCAAAGAAG & Rev Primer CTTATCTCTGCCCACCTACACC (ag) x12 PCR product = 230 bp


UFGT scaffold 01120

  1. Forward Primer TGTGTGTGTGTAGTGTGGTCGT & Rev Primer AAGAGAGAGGGGTCCTGAAAAC (ct) x11 PCR product = 132 bp
  2. Forward Primer TAAGCTACAGTACCTCGCCACA & Rev Primer AAGGATATGCCGACGAGTCTTA (tc) x6 PCR product = 196 bp


EST Search Results

For the second half of the semester, we focused on finding ESTs of our genes using the Washington State University Vaccinium genome database as well as the Towson State University Blue Berry Genome database using 454 sequencing.


ANPER - anthocyanin permease

AOMT - anthocyanin O-methyltransferase

CHI - Chalcone isomerase

DFR - dihydroflavonol 4-reductase

F3’H flavonoid 3' hydroxylase

LDOX - leucoanthocyanidin dioxygenase

Myba1 - myb-related transcription factor

MycA1 - myc anthocyanin regulatory protein

UFGT - UDP-glucose:flavonoid 3-O-glucosyltransferase


1) ANPER Submitted the 3’ most blueberry sequence from my scaffold00666
ggatggcaagctctggtagcttacatcaacttgggatcctattacat
Got this result from BLASTn search of Vaccinium database
>DR067294
Length = 260 Score = 60.0 bits (30), Expect = 9e-11 Identities = 39/42 (92%) Strand = Plus / Plus 

DR067294.png


2) AOMT 01781 search of Vaccinium database
Submitted carboxyl most amino acids that gave hits with my scaffold01781
Did tBLASTn
EEMVLEDLRPSRESTMKLNKMLAADPRVMISHASIGDGITLCMRV
No hits.

I submitted
ATCGAGCTAATATTTTATGAGACcACTTAAAAAaTTTAAAAAaTTATTTtAATATTAATGAACGATTCGGCTCATTTGTACTCTACGGA
From the scaffold

And got
>DW043195
Length = 523 Score = 48.1 bits (24), Expect = 7e-07 Identities = 33/36 (91%) Strand = Plus / Minus 

DW043195.png


3) AMOT 00796 search submitted ATCTAAAAATAAATAAATAATTTGAGTAATAATTATTAAAGACGAAAAAaTAAaGATGACGAAATAGTAAAAGCTTTGGAAGATAAATTTTCAAACCTGGAAACCCGCAAATTACTCCCACTTGCATATATAAGTACTCA
TATAATTTTCTATTATTTAATTTAATGGGTCCTAACATGATTGTAGTTATTATTTTTTTTtATTATTATTGTAGTTATTTATACTACGCAAGATGAAGGGTGCACAAGTCTTCCATGTTGTGGAATATCGCAGCTACAAGCA
TTTATTTAATGGGTACAAAATATTTTTGTAAGCGTCGtGCCCATTATAGGTGAGCATATAAGTCTATAAAGTATTAATTTAGAGAGGTCAATTAAAAAaGAaTCTCCCTGTGACACTACAATCGTGCTTGATGTCAACAAa
TATGaCAAAAAAaTATATaTAAAaTaTAAAacAAgTACTTtCAtgcATtATTtaTTtAAtATTTttCAcACAAATTTGAAATTGATGCCGAAAAATTAGAAATTTCACATAAAATATTTATTTAAaTTAAAAATAGCACGGTTTTTC
ATAAGGAACTAAAATTACTTGGCGGACGGAATACCAGAAAATCACGCAATTAATTCTTCAaGTTTTTCACTTTTCACGGAGGTGGGTTTATGATCtCGTAAATAAGTAGTTTCTTCTTTTTTtATTGTGGATATAATCTCCT
TCTATTTTCTCTAAATATTGAGGATCATGATAAtGCAAAGATGCAAGTGGTGGCTAGCTCATGCGGTAAGAGCTCTTGGATGCTCCATACTGCATCCCGAGATCGAGTCCCATCTCCCTTCCTAATGTAATAAATCAGGA
ATGATGAATGAAAAaTAATACGGAGTAATAATAATAATAATAATGCGAAGACCAAAAATTATGAGGATTTTTTtAGGAGGGTTtCGAATTACACCATCcTTTTTGGTAGGTGAATGGTTCAGTGCATGTGAGCATTTTAAT
AAAATCATTACATATTTTGGtATCAAATGGTATTAGTATATATAATTAATTAATTAAGTAATTATGACCCCTTGCATGTGAATGAAGGTGGTGGTGGGACCACCAACACCAATACCAACTCAACGGCATATACATATTGG
AATCGTCAATAGcGGGCAAGTGTGGTTGGTGGAaGgTTGGTGAAATGAAACTTTaGtCcACGTAACAaGAAAaGAGTTGGTGGCCATCCCcTTCAAGGGATATCATTTTCTTTTtAAAGGGCATaTAAAAAAAAAaTtAAAaT
cACTAACAAGAAATTTTAAGGAAAaTAAaCAaGCCCAACcaTAAAACGGTAgATACCAAaTCTATACCACCTTATAGTATATCTATTTTGgAATTtCACACTAgTGATCAAACGGaTGTGGGgTTgCTGCTATGCAGCCCTAG
CACAACAAGTGCTGTGTATTTGGCTTGTTCGTTACTGGGATGGACggCCcAGATTTTACCtCGGCACCTTTGCATCGAatCGTTTGTtGTCGTGCGTGCAAGTGAAATCTGGATCGTCCATCAGAGCAACGGATGACCCGGA
TGTGCATAGCACCTGCTGTGCACGGGGgTgCACAACAGCAACCCcAGTTCTGATCAAACcAATTCTATTATTTtAAaGATTATTTTATGGgTCTTtGAAaGAAAAAAAaaCTCATTCGGATGTCGTTAAGGGCTTAATAGGAC
GAAAATCGATCAAGCATTTAAtGATATtTGAATGAGTTGATTTTTTataGagACTCTtAAGAAtaTGTTTTAAATCAATTGAAtGACTCTgaTCATTTTTGTGGAATTCCAAAATAATTTTtACACGATAGGGTAAATATTTGGCa
TACACCACATGGTATAaGCAtAGTTTTTTTTTTTttGgtGCCTTTACTTGgTTCATTTCCTTTATTTTTGCGAGTCTAAGTCATTATCGTTTCTAGGGATGGCAACGGGTAAgaCATGGATCAGGGATCCTATTTTACCTTCGA
TCCTTAAATGGGAATTTCCTTTCCACCCCcACcTCCATATCTAAAAGGGaTAGTgTTttCCAcgATACCCGCCTCCCAATGGGGATCGgaTACACTtGGGTAAATtcATATCCAAACTAGTTGAAAAAaaGAACAaTAAGATCT
AAAtAAAAATAaTTtaCgTGTCC

BLASTn
Got 3 EST hits

AMOT 00796.png



4) CHI

I did BLASTn with (from scaffold near SSR primer) TTTCTTGAGGTCTTCCAGGATGAGACATTCCCCCCTGGTGCTTCTATTCTTTTCACCCAGTCACCCCATGGGTTGCTAACGGTCAGTACATCCTTCCACAAGTGTTAGTTCTGCCTTTCTCTTCGCCTTTtGTTCTCTCTTC
CTTGTTGATTATAAAATAGGTAATGCAGTTCCTTGATTCAGGAACAATTTAAGCAGGTATCCAAATTTTCTTTtGAAaTTTGAGGgCAAGGAAATCACGATGATTTATGAGACTGATGTTGCATGTTAATTtCAGCTTGTAC
CCCAGATGCAGCATCGATCAGGACATTGTGAATTTCTTTtGTTTTtGGTCACCATTACTTGGTTCTCTACTAGTTgACGGTTCACTGTGCACTAaGCTCCATTTTGATGGACTTCATAAAACaCGATGCAGGACAagATGTA
AACCAGGCCCATGAAAATAGTTtCGTGTTTTAAATCaTTAAGCCTGTTCTTGACTTCGCCAAGAAGAtCAAACTCGTAGTATAAAGTATGTTGTTGAGAAGtGTTCTGAAAAGTAAGcAAATGGGTTTCTATTTtGGTTTTCA
TCTTTTTATAACACTCTTGGTTAATTTTtACCCTAAAAAaTACTCcATTAaTtAAGACCTAAAGACAAcTGGTTTGGGTtGATTATAGGTTTACTTTTATCTGTTtGGTGACATCACTAGATaGGCTGTGTTGCGACCTTGCCGA
TTGGTTTtCACAATCGGAGCACCTGAGTTATGAAGAGACATGCTTTTTtGAGGAACTTTAGGCCCGATATCGTCTTAACTCTTGAGAATCATTTTCTTCATTGATTTGGACATACACACACATGCATTCGAATCCGTGGTGC
AAATGATTCTGTAAGATACAGGTCATTTGAATCTTCATGCTCAAAACCCCCATTCAATTAGCGACTTACTTCTTACTTCCCAAGGAGCAGGAACTGCTCTTTTAACCCTTCTCCGAGGATTACATCCACAAACACAAAGTC
GATGGCAGACTGATATTACTCATCATCATTATCTATTGCATTTATTTTTCTTGTTGCTGATCATATGTATTTGACAAACTTTGGGATTACATGAGTATGTACATCCACAGATATGCATTGGTGTTGCGCGTGTATGTAGGAA
GTTGCAATGAAACAATAACGTTTTGATTTCCTATTGTTGATGCTTTATTTTCTGACTACAGATTGGCTTCTCGAAAGATGGTTTGTTACCTGAGACTGGCAAAGTGGTAATCGAGAACAAACAATTGTCCGAAGCAGTGTT
GGAATCAATCATCGGGAAGCATGGCGTTTCCCcTGCAGCAAAGCAGAGTTTGGCGGCAAGAGTgtCTGCATTGTTCAAGGAGGCATAAGAAAGAAGGTTTTGGAAAACGATAAGAAAGAAGTCCTGTAGCAGAAAagttAg
TAGtAGTTtATTGAgagaGGAAGATAAAATGGTAGAATCTCACTTCCCAGTTGATTCTGATGTTTCTCCATGAGTAAATGTATCTGTACGCTCTTTCATTTCCGTTTtGTCACTGTAATAAGAAGGCTTCAaCTTTTGCATTCT
AAACTCATCACATCTATCTACGACCTTTTCTTATTCTTCTTTgCAAATTTAAACCAAACcGagCCTTATGtCCCAATAaaTTGGGgTCTACTATTGGGgTCTACTATGtAAatCCcTTTTTttGtCcATTGGgATCTGtGCAAATTC
AAATGATATTGAAATTAAaTTCGGCATAaTGGCGGTAGTGGTTATGGACATCATGAATTAGTAATGTCAAGTGTGGAAATTTTtGCTTGTTTtACTTTGtGTAATGCAAGATGTCGAGGCCAGTATACAAGGGTGGAGCCAA
GATTTTAGGTAAgCGGGGtCAAAAATTAAACGCATATGCAATATAtATTTTTTCtATTTGCTTATGCAAAGAAATAACTAATTTGAGAGTTTAACTATAACGTAAATATACAATGATGCATGTATTTCTGCTTTATAATTGGT
CCCAATGACTTATTATATCTTGAAAAaAaTCACTTATTCAGATTTTTtACTTATTTAATAAAAAAaGTGAGATTATATtAGCTTCAATTTGTAGTTAATTTATTTTAAAGTACTTGAAAATGTTAAAAAaGGTTTGACCAAAAa
GTTTATTTATTTTCAAGAGTGCTTCTATAAATTCCTTAAGACAGGTAATTAATTAGAAGTTATTCTACAACTTATTTAACCAAATAATTTACACTCTTCTTTTAACTTATGAAAGAGCACCAAGCGTATCTATAAATTATTT
ATAATTTTTAACTTTATACTTCTAAAAaTCATCATTTAAAATTTtAACTTATGAAGGAGCAAACAGCTGGgATTGATTTAAAATCTTCGGGACTAGTAGAGCCGCAAGGCCCGCAAAGACTTGCTTGgTTTTGCATACAGA
TTGCCCATTTGATTTCCTTTTTATGGGCCTTGTAATTTGGTCTGATTTAATCAATAGAAACTGCGTTTGTTCGTCCTAAAAAAaGGTTagATAAAGATCCTCTTATTTGATGATTTCTATTGTGATGCAAAACGAAATTACA
TAGCAAGCTCATGTATGTATTGGAGAGTTCAACACTTTATAGCTTTTTACGATAACTCGTATGTCTGGGCTAGTTTCAGTTCACCTCGAGtAATTTTGGGGACcAGTCGACTGTTTACTAGTGAGAGCTCCAATTAAAGCT
AAAGAAGCTCCGTATGAATTGACTCAAAaGAGTTGAAAGCACCTAAGATGTTTCGATCCTAAAATCTTAAAAGGAAGCAAACCCCTAAGTTTTAGGGTCATCAGGCCAACTGCTTAGGgTTAACTATTTATAGCTTTTAA
CTTGGGGAGGTTGTAATGGTTATTTACAATGATTAAAaGAAAAaTTATGCATAACGACAAAtCCAATAATTAAAAGCTTCATAATCCCTTCTCTCTCTAAAACGAAATCCTCTCACTCTAGAAaCCAAAAACCCTTATTTCT
GTCTTtCCTTTtCCcTTtCCCCcTAGGgCTTCACCCATGCGGCAACAGTGTAAGAACCcGTGTTTTTAAATGATTTTTCAGAAATAATGTTGGGAAAATGTAAGGAAACgAAAATAGATTAAAGTGAGCCAATGTGGTGTTA
ATAGATTGCATGTGAGTGCATTTAGAACACATTAGAGACATGTGGCGTGTGTGTGCAATTAAGGGTGTGCATATACATCTTGAGGGGCAGTTTTTTTTTtAAAaCtAAAaCACTTCTCTCTCTCTATAGGgCCcGCTGCCC
CcTTTCTCTCTCCcTCTCCATTTCTCTTGCCAAATCCATATCCCACACTCCACCTCTTTAAAATCTAACCTTAAATCCTTCATCTATGCGTGATTTGGAGTCCGAGTGTCTTTGGTTTCACTTGGAGgAGGTATATTTtCGG
TTGAAATTCAAGAACAAAAgTTGGGGTTTCATTTCTCACCAATTGGAAGCTAAATCTTTGAGGTAGTGGACAAATTTCTACTCCCTcTAGTTGTATTTTGTTGATTTGTCGCATATACCATTGTGTTGCCCACAAATTtCTA
GTCAATCGTGAAATCTCCCTAGAAATGGGTTTGGTTTTGATTGAGTTTGATTCCCACTTGTTTGGTTGTTAAATTCACAAATTGGTGGTTGATATACCCTTATTCCATGCTTAAATGATATTTGAAATTCCAAAATTGACA
TGGGTTGGAACCCCCCTTCATTTGTGAATtGCTGTTAAAAATCTCATTTTGTAGcAGTAGATACCAAGTTAGTGAGGTACGATACCTTTTGGTTTTGTTCACTGATGGCGCGTTGGTATCTATACCAAGTTGGTGAGATAT
GGATACCTGTTGGTTCTGTTCATTGACGgTGCACTGGCATCCATACCAATTTGGTGAGGTATAGATACCTTTCTTTCTGGTCGCTAAtGGTGCATTGGTATCGATACCACTCTTGGAGGTATCCAtaCCTTTTTAAAAAaGT
TCCAAAAAACTCTCATTTGGTTTCCGGTTGACTTATTTGGTATCATACTTGATATATTCACCCCGTGCACTTTATTTTTGGTCGAATTGCGCCTTTCTAACCTACTTtCATATGATTGGTGATAaTGTTGATCTTCCAACGTA
CTTTGACACATatAAACTTTGTAGAAATTGGCGATCTACGTTACATGAAATGAAAATGTATGATGTTTGTTATTTGGACATGTGTGAAATACTTGGTGATTTttGAACGAAAAaCAAGTTGATGCACTAGGATCACTACTCAG
TGAGTCACTAGGAAAGGTTTCACGAGGCCCATAAGACTCGGACAAGGGTTCTACGAGGCTCATAAGATTCGGATATGAGTTCCACgAGGCGCATAAGACTCGGACATGGGTTAAGACTCGGACAAGGGTTCCACGAGGC
CCATAAGACTCGGATAGAGGTTCTACGAGGCCCaTAAAACTCGGACATGGGTTCCACGAgCCCATAAGACTCGGACATGGATTCCACtAGGCCCAAAAGACTCGGAAGAGAATTTCAATATTCTCAAGCTTGAATTGATGT
ACACAtGAGATTGATAAAATGTGAAAATGGTTGAaCGTGGACATTTATGGGATACGCGGATACTCAGAGACACAGTCGGGTAGTTTACGGgCATGTTGGGTTTTCTGTTATCATATGTGGGTCCTATATGGACATTTGACCAT
ATTTTTACCTTTGGACATTTGTTGGTCATTCGTCACATGTTTCGAATATTtCGTAGTATCCATTGGTTGTATCACACATGATAGTCATTCTCTTATGCACATATCACACTTTATACACCTAACGTTTGTAGAATGGTTTtCCTAA
TGGGCTAGTGAGCTCACCCTATCTTTTTCAGGTACATGGCAAGAAACATGTGTTGGATGCTTGGAGTGTGGATGCATtGCATGAAGCTTGTTTGATCATGAAGTCATTTTATCCTCCCCATACTTAGTATATTTTTGGGAGAT
GATCATGTAACTTGTGAACACCCCGCcGGAGTTCACGAAAATGGTTTAAGAAAGCAGTTTCGATTgATTGGA

Got
CHI.png


5) DFR

I submitted the cDNA from grape
ATTTTCTTCCCCGGATCATTTTTGAGAAGTATGGGTTCACAAAGTGAAACCGTGT
GCGTCACCGGTGCCTCCGGTTTCATCGGTTCATGGCTGGTCATGAGGCTCCTGGAGCGCGGCTATACTGT
TCGGGCCACCGTTCGCGATCCAACTAACGTGAAAAAAGTGAAGCATTTGCTGGACTTGCCCAAAGCGGAG
ACGCATCTGACTCTCTGGAAGGCAGATCTTGCTGATGAAGGAAGTTTCGATGAAGCTATTAAAGGCTGCA
CCGGCGTCTTCCATGTTGCCACACCCATGGATTTTGAATCGAAGGATCCTGAGAATGAAGTGATAAAGCC
AACTATTGAAGGGATGTTGGGCATAATGAAATCGTGTGCTGCCGCAAAGACTGTCAGAAGGCTTGTATTC
ACATCCTCCGCAGGAACTGTGAACATTCAAGAACACCAACTGCCAGTGTACGATGAAAGCTGCTGGAGTG
ATATGGAATTTTGCCGGGCTAAAAAGATGACTGCATGGATGTACTTTGTCTCCAAGACACTGGCTGAGCA
AGCTGCATGGAAGTATGCCAAGGAAAATAACATTGACTTCATCACTATCATACCGACTCTTGTGGTTGGC
CCCTTCATAATGTCATCAATGCCTCCAAGCCTCATAACTGCTCTTTCCCCGATCACTGGAAACGAAGCTC
ATTATTCAATTATACGGCAGGGCCAATTTGTTCACCTGGATGACCTCTGCAATGCTCATATTTACTTGTT
TGAGAATCCTAAGGCAGAGGGACGTTACATTTGCTCCTCCCACGATTGTATCATTCTCGATCTTGCAAAA
ATGCTTAGAGAAAAATACCCCGAGTATAATATCCCCACAGAGTTCAAAGGTGTTGATGAGAACTTGAAGA
GTGTCTGTTTCTCCTCCAAGAAGCTGACAGATTTGGGGTTTGAGTTTAAATACAGCTTAGAGGACATGTT
TACTGGAGCTGTGGACACATGCCGGGCCAAGGGATTGCTTCCCCCTTCACATGAGAAACCTGTAGATGGC
AAGACCTAGAAGAAGAATTTCTGTCAGGCTTTCTAGCGAGAGCGTAGAAATGTCCTTCATTCTGTGGAAT
CAAGGTTTTCTGGTAGTTTGATTTGGCCAACTATGTATGAAGTTTGTCTACTATTTTATCCATTAAAGGA
AATTATTCTAATGGTCAATGTGCCGGAAATCAGAGTTCGTTC

Got back from BLASTn search:
DFR.png



6) F3’ H submitted from scaffold (near SSR primer)

ATCAGCCAAAGTCACCAGTCACAGACTATCAGCATGCACCCTGGGCCAAGATCAAACTGTCCGTGTGCAATTAAGGTTTGATTATAAAATCCCAACAGTTAAAAACTAAGTAGTCATTACATCACTTtGGCTACTTATATATTCA
CCTAGATAGAATCAACCAACAGGTTcGTATGGCGATTCAGAGACACATGAAATACCATTGATGACAGGATATAAACACACCAACACAAATGCCTATtAATACAAATGAAGAGCAAGCATGAATTTTGTGCACATCATAAGT
TCATTAGCTAGGgCATAAATGGATACAATCTAAACGAGTCCTCAAAGTGCCGGCATTTtGTCTTGTTTTTGTCATTATTGTAAGAGCTGCTTCCTGCTgATAGTGCATTcTCTCCAAACTAAACCTACTAGGTACAAAACCTT
CATGGCTTCTTCAAACCTGGGTGGCTACCAAAGTACTACTCACCATTACACTAACTGTACTGACAACTTTCTTTATCTTGTTAAACCTGCTGAATACTTATCATTTGTAGCTTGTTGCTTATGTTAAAGTTGAGCTTGTTAA
CAAGCGGCAAGGCTTGTACCATGATTGGGTTACTATGAGACTAAGAGCACATACACGTCTACCGGCCTCCATGTGACACAAAGAGCAGAGTGACAATGCAAGGAGGTAGTGCATAAATGAAGCTTACATTGGAACTTTGT
AAAGTTTTAAACTTCAAAAGATGTGTTTTCTAAGAGTTATGTCTAACTTTTAGTTTTGAATGAAAAAACATGACTTTGCGAAAAGATGTTTAGAATCCTATAAATATAGGATCACCCCACTTACTTAGCCTAAATGACTTTT
AATTCTAACAACTCTCCGTATTATTTTCATATATTATGGGcAaTTcGATTCCTTCTTGGCACCCCCGTCTtCCACTGATCGTGTAACCTGGAGACAAACTGCGAGATCTATGGAAGGTATCTTGGAGTCGTATTCAGCCTTTG
GAACCTTCATGAAGGGAGGCAAATATGGCTTTAGGATTTATCCAAAAATATGGCTTTAGGACAGcGATTTGTCGTGCCTTTCATCGATTtCACATGCTAGTTTCCTAATTCTATTCATATTACATATTTGTGTGAttGTGTTCA
TATTTTTAGTGTTCAATGGCATTTCTAATTTCCTAGTGTTTTGTGATAATCATGTGACAAAaTCCCAACAGCTTATTCCATTCTAAGGCAGTAGTGAAGGAAACGACAAGTCAAAAAaTATGGACACAACCCGACACTCTAC
GGACAaCTGTCAAATACACCTAAATATGTGTCTAATTTTTCTGTACTAAATTTTCAAATGAACATGTTGTGGACTCTATAGGAAAGGTtGGGGATTCTCTGGGGCACGTTCAAGACACATAAGGAGTTAAAATGCAACTTTT
AAAAGTGGAGTCAAAACTTCCATTGCTGTTGAGTACATTAATATTAATGTAGTAACCATTAATTGGcATAATGTTAATACTATTGGTGTTTTTTGTGTTAATTtGATAGAAATAATCTTTTAACTTTTATACGAATTCCTACG
AATCATTGACTGATATAAATATACATTATATATtCCACGTGCCcTAACGTGCCCgAaTCTTAGTTTTTACGGAGAAGACATCCGTGTCCATTTTGTGTCCATATCCTTGCTTCTTAACTGTTAGATAATGACGATcAAAAATT
CGATCACTCCAAcGATTGAGACACTGCGTATAACACCAAACTCTCCACCAACTCTCTCACAAGTTGATGTGTCCCACACcTGCGGGGCTTGTGCGAGGGAAATTGAGCTGTTACAGTCTAACTAACGAATGCCAATTCAGT
GGGCTAATATTTAAGTTATTGTGTTCGATTTCTATGTTGTACTTCCATAAaGAAAAATTGGTGCATCTGTTGGAGTTTGTCCCACATTGGTTAATTAAAACCTCCAAATCTAGTATATGAaTCTGGGCAGCCTCTCCACTCA
TTGCCAATTGGTTTTGAGTTGGATGCATTAACATGGTATCAGAGCTAAGCTTTCgCAGGCTTTGGACCAATCTCTTGATTGATTGCCcTCTTgTtaGTGCGATAAAGTGCACCTTGTTTCAATATAATCCAATGTTACGGATC
TTTGTTTACCTCTCCCCATGCGGGAaCTAGACAGGGGTCGCACATGCGGAGAGAGCATCTAGATCTCAAGGCAAGTTGAAGTGAGaaaaaaaaaaagaaCAGTTTGTGATAAGCAGCAGAACTGAGACATCAGATGCTTGCTT
AAAAAACACCTTTCGATGTAATAAACAGATGGGCTGCCAAGCATGTGAccTTTTAAAACAGCaGTTAATAACATAGAAATCGAAGGTCTCCTATCTCACTACAATATCTATCCCCTAGACAATCCcCCTAAGTgTCTCTTtCG
AGAcaGTTTTCACATTATTATTGTTATTGTtCCACATTGCTTGGGTATGGAATGGGAGATCACTTAATAACATATGGAACCTCTCCACTAATTGCCAATTGgTTTTGAGTTGGATATAAAGTTTCCAAATGGTATTAGAGTGG
gCCAATTCCATCCCAAATTTCTTAACAAAAaTTAAATCAAGCCCACACCCCACGATGACAGACCCAGGAAAGGTTGTACgATGATAGAACCCAAAAATTGGCCACAGGTGACAGACCTAAATGTGGCTGCAGGTGAGGGG
GGAGTAAGAATAGTTGTCTCATATTGCTTGGGTATGGAATGGGAGATCACTTAATAACATTTGgAaTCTCTCCACTCATAGCCAATTAGTTTTTGAGTTGGATATCCACAATTATTTCAACATTGACTCAACTGAGCACCTT
TATCAATCTTTCATATCGACTCCCGTGTACAAGAGGCAAATCAGTGCAAAATCACCATAATACATAAAGCCAAAGCTTTCAAATATTCAACCACACCTGATATAGTCTCAAAGCATTTGAATTTCCAATTAGAGCTTGCAG
ATTAGACTTGGGAAGATCCAGCAGTTGTTGCACATTTGAGAAACCCCTTCTGCTTAGGGAACCGAAGAGATCATCAGTCATGCATGGCAACATCCATAGAGCAGTGTCACTGCCAAACCATAAACCCTGCAAATGAATCC
AAAAATCCATCAAAGTGTTGCATGAAGAGGAGAAAAATAGCAGAACCCACAAAACTAAACCTTCAAATGGAGGCTGTTTAAATCTTCTTGATGGACAGAATAAAAAGAAAGACAAGTGTAAGAACCAGGACACAGCTCCA
GGTTATTTCCAGGAAACTATTACTGCAATGGATAACATGGAACaatttATGTTCGAGAAAGAAAACCAGGTCACCTGTCCTGCAAATAAcTTTAGAAGTAGCCAATTTCCAAGGGACCCCGTGaGAAACCCTCGGCCGTCCG
ATGGTGTTTTGGACGGCCCGGATGTGTTAAaCACATTTGGGCCaTCCAAAACACGATtGGACGACCGTCGATAAGAGTGGTACATAAAGAATCGTTTATTCCCACAAGAAGATGCCTATTCAAATGGTCGAATACAGTTCC
CTAAAAGAACTTACAACTTACAAACTTTGATAATCATCCCTTAGCTGTGGAAAATAGGTCCGATTCACTTTCTTACCGAAGGGCGGGgAGGATAGATTGATTCAtCTAGCCTGTCAAGTAGAGCCCCCTATAGACGTCAAG
ACTTGACGGCCTAAATGGGTGTATCATGCCTTGGTtATTGATCCCAAGGCCCAATCCAGGATGAAAACTCCATCTATAGATCTCTAATGAGgACACAACCTTAACAcGATGGCCAAAAaTAGAAGCTTGATGCGTGAAACT
TCTGAAGAAAAAATTAACCCAAAAAGTTGATGTGTTTCGAGAAACATAAAGAAGCAATGGCTAAAATGACAAGATGATATACTGGTAGGTACAATAAACTACAGtGGGTAAATACAACCAACGAAGAGAGCCTCCAAGTTT
AGATCTCCCTATTGAATATTGGAGACCAATTCGTTGCTGCCAAGGTCAAAATTTGAAAGAGGCATAAACTAACCTAAGAATAGAACAAAACTTTTTATTTTGACTTCTGGAGCTTTGGAGGTCTAAGATAATCCCAAAGTC
CAGCGTGGTTTCTCTGGTCACAGAgaGAAATTTTTCCTAAACgaTACAAGAGATAAAGATGAcAACTATTAACTACCAACAGGGATAAAACCATGGTAAAAATACAAACTATACATAGATTTTTGGGTTATGGTTTACCCAC
cATTTTAATAAGTGCAAGTTCAGGAAAAGGTATACATGTATGTACCTACCCATTAATTCAAATTGCTGCACTGATGCGATTAACACAGAAACAGAATGTACTAGAAATCTTTCACAAGGGAAAAAAaTTTGCTTGGCCAGA
ATCGACAAAGGCAAGTCTAGTAGTACCAGCAATAACTTGGTCAATACAGAAAAATGCAACTTTTGCATCTTTTCTGAAATAATTGTACTATATTCCGCTTGTTTAGGTCTGTTTCGCTATTGGgTTGTCTTTTTGTGTTCAA
TGGAAACATAAAGCTTCATAATtCCACCCGTGGTTCCTTTCGGTCTCTTAGGAAATGGAAAGAGTTCAGCCATTTCTACAGAAACAAATATGTATTAATTTCATACTTCAACGAATGTCGCTTTAACgACATACCACAAATT
CACCGATGACTTAATTATTCTGGATTCACAATGACTGACTTAGCATCATATCGAGAAGTGCCTAGCAGTATCTGTGAAAGACCAAACATGTATTCcTGTCCTCATAGATTgttGGTACAAAACTTGAATTGATCGGGAAAGA
AGTGCCTGGTtGGAAATCATAGCAAACAAACTATAGTAACAGCCTCAAGTGGTGAAGGCATTCCCAATGTAGATCCTGAAGAaCTTATATCAGACAAATGTTGCATTAACAGTCAAACTACAAAGTTGAACATTTTGTACT
TAGTTGCATGATGGACAATATTTTACCTATCATTCGCAATGAAACTGGCAAAATCACTACATGGGCGAAATAAATAATCAACTCTTCTAACTAGTTCAATgTAATTTCAGACATCCAGCGTCCACTGTCCACTCCCAATTTT
CTATAGaTATGAATAGGATTATTTCATACGACATGATAAAAGAGGTCATCATAAAAACGACGAAAAACTCCTCATAAGAACATTCGGcAGAATGCAATATATTGTTTAAAAGCAAGCTTTTAACAACAGCAACCATGTGAT
TGATGAGACTGATGATTAAGATGGAAAAGACAGTACATACAGAATTAGAATCACCATTCTATTCTTATCTATCAGACCGTTCATCTACTGGGAAAAAGCAAAAAAGCAACGACCGGGAGAAATACCTGCATGACCATTTG
AAGAAGATGCATACAGGTGATTGTACTTGAAAGCCATCCACTGTTCGCACATATATCTATCATTGCCTGTATGATACGTATACTTTGATCGAGCACAGATTTCAAGTCTGTGACATAGTCTGTGATtGGTAATTCTAACTGA
GAAAAGTGGGCCTGCAGACCAAACATCATATATTTAAGAAGATAAGACCCCAAGAACAAACACAGAACCATGTATTATATCCAGAAGACAGTCAAAAAGTGATGTCGACCTGGAAAAGTAGATTCGCTTTAACATGTGGA
TCATCTAAACGATTCTTATCCACCATATACGGAACTTTCACAGACAATGCTTCATTGTAGTTTTCCTAGAACCAAAGAGAGGATGATTAGAGAAAAAGTCAACAGACATTTCATGTGTTATTAATATAAACTTTGAAGATGA
AGCCACGACAAACAGCCAATAAATCATATAGCCAATAAAAGAGTGCATCATCTATGTGTCCAAGAATtGGATTCATCGACTTCTCAAGCCCAAAGACTAGGGAACATCAAACATTCAGATTTTTTTAATATCATTCTTTCAT
GTGCTCTGGAAAAACTCTCTTCACAAAaCATAGATCATTACGCATAGTTGTTCAGCtcTTTtTTTTTTTtAAAaGCAACAATGTCAAGgAaGTAGCACTGTTCCTGAGTTAAAATATACACCAACACGTTAAACTGAGACAGCG
AGAAACCCTtCCTGGTTGGTGAAAGCAAAATCAGAATTTATTAGTTTGATAATTAGCAAAAAAAAAaGATGATCACAATTAGAAAACCACCTCATTATGCCTCACAGGAAGCTCATCATATTCGGAAGAACCAGACAGTAT
GTGAAGGAAAACCTGCAACGAAAAACAAATGAAGTGAAACATTGCTTAAAAAGGGATGAAAATGGAAGTTATACCGGCTATATGTTGGAGTTTTTACTTCAAGAGATGTATCCGGGCCTATATTTGAACCAAACATTGAA
ACAGTCATGTACTTCAGGTAATACTGAGAAGCTATTGAGCCCAACATCATAGGTTCCACGCTGTCTTCATTCATCTTCAAACACCCACTGTCTTCCAGATCCTCAAAGGTGTTTTGCACCAAGCTGCCAAACAAAACGGTT
ATAGAAAATTGCACTACAACAAAACCTTGGGTCATACAGTAGGTTATTACCTTGACAAATATGAACTCACAATTTCAGGTCCAGTATCCtCTAAGCCATAGTAAGCTGGATTAACCATCTGTCACAGGGATATATTTTTTGT
AAGAAACTTTAGCAGGGAAACAAAGACAGCTGAAAGCCCAaTGCTGGGTATAGAAGCTGTGGTGTGATTCCATCCTCAGTCGATCATTGTTCCAAAGATGAAAATATCATAAACATGCAcAATTGAACAcTATAAATGCAT
CTAGTCCATTATTTCAGGGAACATGACatGCATGTTTTGGGCAATCACTACAATCATTACGCTGAAAATGCAAATTGAGAAtAGCAGGTATCCAGGTCAACTTCGTGCTTATTATGAGGCATACATGGCATCATTATTTTGC
TAATAATGTTAaGCAAGAGAGTGTCTCTACGCATTGCTTAAGTTGAGACACCAAATCGAGCCTTCCACATCACcGAACTCGATAATCATTATAGACTAACAGAGTTTTAGTAATTCACTCCATACGCTCTGCTTGTTATCAT
CTACATAATTATATTAATGGGTTTGATGCCTTAAATAAATCTTTCCAAACAGCCGGGAAATAACCAATATTTTACTAGATTTTAATCTCAACTTCCAAGGGTACAAAACATTTAAAAAATATTTGGTATTGTACATGACCAA
ATAAGTACAAAACAGTTTCAAAAACTTTTCTGTTGTTTTCTCAAAATTGGGTTGTCTTTAGAGAGAAATATTGTTTGCCAAACAAGATTGGTGTTTAATGGAAACGGAAAATAGAAAAGCAAAACAAaCaaaacaGGTGATTA
GCTACCATAAGTTACAACTACTGTTCAACTTCAAAGAATTTAGCATTTCTGTTTAAATTAAAAGAATCTAATGTTAATCATGTGAAGAAACCAAAGAAGCaCTTCCAACAGCTAAACTAACACGGAAAGTTAACAACCTGG
TCATATGCATTTTACATTTTCCCCATTTTACAATGTGCTCCCACAAAAATGTGAAAACGTGTTCTTATGTAACCTCTCTTCAAGTTTCGGATACAGGACTCTGCTTGTTGACATCAACCTTGCATAGATGTTCAAAATATTT
TCTGCCATTATAAGACAAAAGAAGACCATTTTTTCTCTTCCCAAACTTTTGTATGCATCTTCCCAATATTTAAAATGTGCCTCACATCCCAAGTGTAAGTGTGTGTGTGTTTAACACTGGATGTtCTTTCCTGATAGGTGTAA
GTGAATGTAATCAACATAAATATAGTTTGACAaCCGAGAGAATGTCCAATATATCTATAGAACACAAAACAAGAACTCCAGTCTCtAGTACACGCTTTATCCTTCTGAAATGAGTATAATAAGAAAATGAGGGgAAGCCTA
GAATAAGTCAGGACCAAGCAAGGTGCTTTAAGCgCTGATTTACTTATTGCGCATAAAAAGGAATTGCcGAAATTTTACCAATCTCCGAAACAAATAAGTCCAGGTGAGATAATGAATTGCATCCTCTCTATGGCAAATTGT
TCCAGAAACTATCTCAGCATTAATGTGGTCATGCAACTGCTCCCTCAGACTACTTTCAACTGGGAATGGTTCATACAGAAACTGCAACAAATAAAAC


F3 H.png


7) FLS
submitted from scaffold near overlap sequence with query
GAGAGAGAGGgaGgTTGcTGCAATCACGCTCTAGTATCGGTtGTGAACTCTGCCGCAGGAGAGGTGCGAATGGTAGGTGACGTCagCGGCGATGCGaTGCTGGACGCcGGTGATGGGACTGGGAGGGGCTTGGGGTTTTGAAGGACCAAAGGGAGTAGACCATTAGGAGAGTtAGGTGGAGGTCC
AAGAGTGTCGTCGTTTGGGTCTGGTGGGTTGTTTCGCCATGGAGAGGTGGTGGTGGTTGGCAGTGAGAATGGGAGAGCTCAGGCGACATTATTTTTGAAGGATGTTTtAGAGGTGGTGGTTATtAATATgTGGgTTATGTAGAGGAGTGGGgACGGATACGACACAACACTCTCCGGACACG
TGTTGCTTAAATACTTGTCtAATTTTTtCTTGTATTTCTTTTATTCAAACACATGGTAGATGTTCAAGGACACGTTGGCAAAACTCATAGAGCGTGCTAAGGACACATAAAAGGTTAAAATaTAATTATTAAAACTtGGCCATAGGTGAAAATTtCCACCGATGTATAGTCATGTATACTTAtgCg
CAGACTGCTAATTTATCTTTGTTGtaTTaTTTCTAAATTAGATAGAAATAAAGATTTCTAATTTATATGAATTAATCACATACCTCTATGATACAAaTAAaTTTtATATTGGACGTATCCCCAACGTGTCCTTGTCCTAATTTTtGAAGAAATAACGTGTGTGCGTATCCGCATCGTGtcGTGTTCG
TATCCCgTATCCGTGtCcGTGCTTCTTAGACAATAAATATTGATCgTCGGTAGCCACACAGTTTACTAAGGGGCAAAATGTCTAACTAGTTGGATTCAaCTATAGCAGTAGCCGCTAAAACTCTGTAGGCTCgCTCTCTACTCCTATGGTAGCCTATGCTGCTAACTCAGTTGAtGGaTAGCAT
GGATCCcATAGAGCTCTCTCAGTCTCTAGCCTTGTTTATGGCTCAAATCCCTCCTCATCTCCAGTTTCACATCACCGGACAGCTATATAGCCAAGCCTATTGTATTTTTtGTATGAACACTATGGAATCTTTCATTCCgAaTTCAACACGGCTATCCAGACGGAACCTGAAGTGAGGCTTTAAT
CTGGGTTCTCCCATACGTACCCTTTTtATTGAGAATGCAAGGCATTTACGGATTGACATCCAAAATAAGATaCAGACATGGGACAAAGCTATGTGAATGCCGTGTtAATTAATTGGGGAGCTATTCCATTAGTATTAATTTTGTATACCACAGAAACTTCTTGCCTCTAGTtGTCCACTTTTCCC
TctcATCCTCTCATTCTGGGTGACCCTTATATGTGCATATATATAGATATCAACCCACAAACGCAAGGTTGATTAGTGATCATAGAAaGAAAAAaTGATGGACCAGAAGGAGAGAGTGCAGGCCATTGCCTCGGTGTCAACAGACACCATACCGGCAGAGTTCATCAGGCCAGAAAGTGAGC
AGCCCGGACTCACAaCCTaTGGTGGGCAGGCCcTTCAAGTCCcGGTCATCGATTTCTCAGACCCGCACGAGGAAATCCTCGTGGGTTCCATTGTCGATGCAAGTTGCcAaTGGGGAATGTTCCAAATTGTGAACCATGAGATTCCAGTAGAAGTAATAAGAAAATTGCAGCAAGTTGGgAAA
GAGTTTTTTGAGCTCCCACAAGAAGAGAAGGAGGCTTATGCTAAGCCTGTTGGGTCAAAGAGTGTGGAAGGGTATGGAACAAAGCTGCAAAAGGAAGTGGAAGGGAAGAAAGGTTGGGTTGATCATTTGTTCCATAAGATATGGCCTCcTCATACTATTAACTACCAATTTtGGCCTAAAAa
CCcACcTTCTTACAGGTTCgTGATaCATTTtCTTCTCTCTCGATTATTCgTTAGGGgCAGATAGAAGGgCAAACAaTTAGAGTACTACTGTTTTGgCAAAAAaaTGGATtATTTTTTTGTCTTTATTCAAaTAAAATTTGCATTtgTtAGTTTTGCGTTGATTTTTTGTGGATTATTagTTCATCTCGA
CAAGATGAATCTAAAAAGTCAAGAGTTACTATCAAAattaaaaaaaaaaaGTTCaCTAAAGACAATAATtCAAAATTTtCTCAAAaCACACATTCATGCGATTCGACATTtGTCATTACGCATGCGTATaTTGGGAaCATcTGATCTGCaTTTtttATTttGgTtATTTTtCTTGGTGTcTTATAAAAATGTT
GtGTCTtGTt

CV091044.png


8) LDOX submitted from scaffold AAGCTTCGTTTAATTTTTCAATATATAAGAAATGCCAAAAAATGGTACATTGAGACAGTGGAGAGGGAGAGAAATCCAACCAAAAGTTAAATAAGAAAAAATTAAAAAACCACCCAAACATTAGACACCCGAGTACCCAACGGCCTCAACCTTATCGCCTTTATCGGTGAGGTAG
AGACCGTGTCAATGCCACCGCTGCAACCCACCACCAACAATCGTTTTAAGTTGCATCCAATTATGTTTATTTCCATAAACTAGCATAAACATTTTTCAATTTTTAGTCTACCTTTTAGGTATGGAATTTTTCATGATGTTATCTTGAAGTTTGGCTCTACCGACTGACTAAAT
TTTCGTGGCTCCGCCCGCGCGCAGGTGCCAACCATTGATCTAGAAGACTTGGTATCGGAGGACAAAGAGGCCAGAGAGAGATGCCACGAGGCGTTGAAGAAGGCGGCGACAGAGTGGGGGGTGATGCACCTAGTGAACCACGGCGTCCCCGAAGAGCTGATGGACCGG
GTTAGGGTGGCCGGAGAGGGGTTCTTCAATCAGCCCGTGGAGGAGAAGGAGAAGTATGCTAATGACCATGATACTGGGAATTCTGGGAAGATACAGGGGTATGGGAGTAAACTGGCAAACAATGCCAGTGGGCAGCTGGAGTGGGAGGACTATTTCTTCCACACTGTTTA
CCCTGAGGACAAGAGGGACATGAAGATTTGGCCCAAGAACCCATCCGACTACATGTAAGTTTTTTCTGAATAACGTCCATAGATTTGCTGTTTACATTAACGGGTCCGAACATGATGTATTTAGAACCATTCTACTCCGATAAACCTGGTGGTATAGAAAGGGGCGTACAA
AAAATGTACACAGCGCGTTTGGGGACCCATTTTTGGGTTCCACATGAGTGATCCGAACCGTTCATTGTTCTAAAACATTTTTCTGAGGGGGATCAAACAAATCAGCTCAATCGGACGGTTAGAAGTGCTTGAACCAAAGTCCCACCGTTTCAAATAAAAACTTTTCATTTGA
AACGGTGGGACTTTGGTTCAAGTACTTATTATAACCATCCGATTGAGCTGATTTTTTATGGGCCCCCTCGGAAAAATGTTTTAAGAACAATGAACGGTTCGGATCACTCGTGTAGGACCCAAAAGTGGGTCCCCAAATGCGCTGTTTACATTTTCTGTACACCTCTTTCTGT
ACCGCCAGCAGCGTCCGTTCTATTCAAGTAGGAACTACAAAAATTTATTCCACTAAGACCGCACATATCTCGAGTAATTTTGAACTTAATTTGCAGTACAATTTACTAATGTTTTATGCACCG

LDOX EST.png


9) MybA1
did tBLASTn using amino acid results from scaffold03500
LGVRKGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG

Myba1 EST.png

10) MYCA1 submitted from scaffold 00278 (BLASTn) tacattattttcctttctttctttCTCCTTTCTGACTTGATTGACaTAGTATTAACCGATACGATAGTATAATAGAaCAAGGGATCTACCCTGTTCCCTTCTCCTTTACGGTTCAGgAATTCATCTCCATTAGTCATGAACCCCGcTTGgCCcGCCATTGAGTCACAACTCTTCTtGTACAAACTTCTTTCAACTgaTCAGTCGATAGGAAAGTCGGATGACTCAGATGCCTCTACTGTAAGTaGTGGGAggtGCATAATtCATTATACGGGGGaCCAAATCACAATTTtgAGGGAACATTCCTTCATAATTtATACAAACAAAACCTAAAATATAGTACCAATAATGAATAATTGGGAGgTGTGCCTACCCGGGCCCTCGTATCCACCCTACCTGcAGGCTGAAATATTTCCTTTGAATATGTTGATGTAGTTCcCCTCAATTTTTTATTTTTATTTTTTAGAAATATCACCGTTTGGAAAATCTCAAATAAAgAGTAGTGGGATATAAAATAAGGAATCATTTCCTCTCCATACTCAATCCCTCCATgTCTTTCCTTACTCTTAATATTCATTCATATTtCAGGGCCAAAACAATGATCCTAGCcGTTCACATCGTAGAACACaTAGAAAAATATAGGTTTGCAAAATATCAATATGATCATCCATGATAAATTTGTGAATAAGTTACCTTTTGTTTCGTAGATTGTAAGGTTAAACTGTAGAATATATTTGCCATTCATTTTGCATAACAATTTGTAACATGTTTACATTGTCATTTAAATTTGACTTTATATTTGAAACTTTATATTATTTTAATTAGTTAATTAATTTGTCTTAACAATGAATAATAAAAAAAaaTTcTAAAaGTGAaTAATATTACAATCACGTTTAAAaGTGAAAAAaTaTTACAATCAACGTTGGCAATGTTCAGCCCTAATCAATATTGCCTCCAAATTGTGGTCCCGGCACAaCGCCCAGTACGTGGAACCACAAATGAAGGCGCGTGTCATAACTTAAAGCCCATCCTTTCACTTCATCCGACACATGCTCCCACCActATATTGGGATGTGTGGTACAaAGTGATGAGGGTGCAAAAAAAGTTGCACGAAATGATTGCTAATACGGGCAATAGCTATTTCTTTGCTCTGTTGGACTGGAACTGGAaGGgATCTCAaGGgCAAGTGAGTATAACAACCTCCAACATTCACGTCAAATTTTaCAGCACAAAGTTGTAACGaGATGCAATGACTACCgCcAAACTTATACAGTCCATCCAATTTGATTCTGGAGCTGTAGGTAGGAAATAACtcAGTGACAAGATATTAAaTCATTAAAAAaGAGAAACAAGATATTAGAGCATATTAAAAAGATAAAaGAGAAAaaaaTATtAATAAAAaaGaTAAATAAATAATtATATAAAAGAAaTGAATTAAATAAAaGAATTGAATTAAAtAATTAAATGAATtAGTAaTATAAaGAAATtAATTAATATAAATAATATAAATAAAGgAGGGAAGAGGGgTAAAaGGGTAAAAGGACAACATAAAGGTGGTGGGACAACATAAAGCTTAACTGAATCAAGAATTTGACTGTTCTATGTTCAaaaAAAAAAAaaaGGgAaTTTGACTGTTCTAGGTAAAAATAaTGTCTTTAAGAGCTCCCAAACAAGAGAACTTATTATGAAGCTGATAAATAGTTGAATTTGCAGTCTGCCATTGCAATTCCTCCTCAACCCACCaTAAAAGCAAGTGGGTCTTCTTCTTTTGAGCTTGAGAGCAGGATCTGATTGCAGAAATGGAAGATTAAGAAGAAACGCATCAAATCCCAGAGTGAGTTTCTTATGTGCTCTTTAACAAGATAGTCTTCTCTGAATAATCTACTTTCTTCCTATTTACTTCCCCCCCcAAAAAAAaTCTACTTTCTTCCCTCCAAATGACTTTTTCTTGGTTACAGCCTTTTCCCCTTTTCACTGTGGTTGATGGCTTTTTCTAACCTCAACATGACAACATCAGGTGCTTATGAAAACCCTGATCTTGATTTTTTATCTTATACAAAAGCTGAACTAAAGCTACTTCAGAAATCCTAAAGTTCTCCTTTAGAATGGCTTTTCTAGTTTTTGCTTTGCATCTTTTGACTAACTGAGTAATGGGTTTTCTTAAAATTTGGTGACtaGAGTTTTGAGGGTTATGTGTTGGGgAATGGCTAAAGGGGCACAAAACCAACAAGGGGTGCCAGAAAATCTGaGAAGGCAGCTTGCTGTTGCTGTTAGGAGTGTGCAGTGGAGCTATGGAATTTTCTGGTCATTGTCAACCAAACAAGAAGGGTATGGTTTACCTTTTTACTATTTTATTTATCTTCATATTTTTATGGTATGTCCTTAAACATTCTATTTCTTCAGTTTCTGTTGCTGGTTTTGCATAAATTTCCAGATCTGGGAAGAATAACGTCTAGTTATGCTCATTATAAATGCTTCCCATTCCATAAAAAAaTTGATTTCCCTGCTCATTTATGGCCTCTTTCCTGCAAATGTTAATACGCCTGCTGATTTATGGCCTCTTTCCCTGCCCAATATTTATGTTCATgTTTGCCTAGTATGTCATTACATAATTACATTTTCTATTTCTTCAGTTCATGTTGCTGTTTTCTTCATTATTTTTTGATAAAATAAAATAAAACAAAATTTCGTTGagAAAGCGACGAACGAGACCAAAGCTTCCCTTACAAAGGGTGAAATGCTGAAAGGGAAgAGATTGAAAGAAAAGTAGCTTCAAAGTTCAAACCTAACACAGGATAATGCTTTtCTAGGGAAATCAACAATTTTCATGGGAAAGCCATTGCTTATAGCAAAGTACACCTACATGCCTTGGTACATTGAAATTCCTCATGCTTAACTGATAAAaTATGTTCCTTGTTATTTTGTTAAGGGTATTGGAGTGGGGTGATGGGTACTACAATGGGGACATAAAGACGAGGAAGACAGTGCCGGCCATGGAACTTAAACCAGATAAAATGGGTTTGCAGAGGAGTGAGCAACTGAGACAGCTTTACGAGTGTCTCTTAGAGGGTGAAGAAAGTGATCAACAAGTGCCTTCTGCCGCACTGTCTCCAGATGATCTCACAGATGCAGAGTGGTATTACTTGGTTTGCATGTCCTTTGTGTTCAACCCCGGCCAAGGGTACAAGCTTTCTTCTGGACATTTTCATCTTTCTCTGTGgCTGTGAAAACTTaGTTACACTGAAAACTAAACAGAAAAAACCGAGATGTGACCGTTCAAATCTAAACAGAAAACTTAAAGTTTTAGAGTTTCTGAACTCTGCAGAAATTCCTGTGTCCATTTTTGTTtATGAACAAGAAGTATAGTAACTTGCAAATATtATGGAGTAACCGATATTGGATCATTTGTGTGTGTTCATTAAGATAATGATCAGTTTTTGAGGAAATTGCTGTGTAGTTTGCCAGgAaGGGCATTGGCAAATGGTCAAGCTATCTGGTTATGCAATGCTCACAGTGCTGATAGCAAAGTGTTTtCTCGctCTCTTCTAGCAAAGGTCcGTTCTTCTGGGGCTGTGCTCCGTTTTAGATTTCGTGCCAATTTTAGCTTGTTTACTAGACAAGTTTtACGATATtCAGCTCAATATACTCTCTACTAGTTAACTAACAGTGATGCTATAATTTTCTTCCTTTCTCAAATGGTTGGACTTGCGCACGAATCAACCTCAGAGTGCATCTATTCAGGTAAGCCGTTATTTTTATTAATTGGTACGCAAGTTTGCAAGCACACACATTCACAGGCACACGCACATAAGGACGTTGGTGGCCCCATATAATAGATCAAACAAGCAGTGAAGAATTTGTTTCCGTGGAGAACGTTTTTTTAGTAATTTTACTTGCAAAAGCTTTTCATAAACGAATCTTGTTGCATATACCGGTAGTCTACTACAATGACCATTGTTTtATTGCAGACTGTAATTTGTTTTCCCTATTTGGGTGGAGTCATTGAGCTAGGCGTGACGGAGCTGGTAATTTCTGTTCTATTGCTGGATTTACCACTATTTTTGTATTGAAGATGCATCAGATGCTATACGAATTATCCCAGAAGTAACTGGTTCTGCTATTCTTTTGTTTTCcTAAGGTATTGGAAGACCCTAGTCTCATTCAGCACATCAAGACTTCTCTATTAGAGTTTTCAAAGCCCGTTTGCTCTGAGAAATCTTCTTCTGGTCCTCCCCACAGAGACGATGACGCTGACTTCATGTATGCCAAGGTTGATCGTGAGCTGGTAAATACAATTTCGTTGGAGAGTTTCTATTCCCCAACTGAAGATATGAGATTTGATCGGGAGGGACTAAATGACTTTGAAAAAAATATCCAGGCCGATTTCACTATGGGTTCTCCTGACGAATGTTCGAATGGGTGTGAACACAATCACCAGACAGAAGACTCCTTCCTCCTTGATAATGTAACTTCTCAAATGGAAGATGATTTTAGCAACTGCGTTCAAGTTTCCATGAATTCTAGTGATTGCATATCTCAGGTCgTTGTGAACCAAGAAAAGTTTATTTCTCCTCCCAAGGGTGACAATAGTAAATTGAAAGAACTTCAAGAAGGGAATGATACAAAATTTGGATCCTTGGACATTGGAACTGATGATGACTTGCACTACAAAAGAACTCTATCTGCTGTTCTGATAAGAAATTCTCATAGGTTACTTGATAACCTTTGCTTCCATAGGTGGGATTatAAGTCTAGCTTTGTGAGTTGGAAGAGAGGAGAAATCAGAAATGGCCATAGACCACAGGTGCCGCAGCAGATGTTAAAGAAGATATTAGTTGATGTCCCTcTAATGCATtGTTTCTCACTCAAGTCTCAACAAGGAAAtgTGGGGAAATTTtGGTTGTTAAAACAAGAAAGTTATGTGCAACATGATTTGCCGGATGATGGAATAGAGAATGAGAAGTTTTtGGtCCTCAAATCGATGGTTCCTCCTTCTGTTAGCAAGGTAATTCAACTGCCATCTATTGGAAAATATGATCGAATGACTCAGAATTAAATATTTCTTTACACTTCTTGCTCTCAAGACTTGCTCATTTCTATGTAGAAAATCAGGATGGCAGAAGGCATTGACTTAATCCCCACCCTCAAAACAGAAGGATTCGAATTTTCTGAAAAACAACTTTTCGATGTtGCTAATTTTAGTCACTAAAAGTAGATTTTCAAGAATAACATAATTTTCTTGGGGTGATGGTATACTATATTTGaGTCCTCAGCATGTCACATTACCTTCACTAATCAGAGGGAACATATAAACAACATGGAAAAACGCACAGCAAAAAAGTAGGAAACGGGAAAATCCTAATCTTATTCATCATGCTTTGCAGGTTGACAAAGCAGCAATACTTGGTGACACTATAGAATACTTGAAAGAACTTGAGGGAAGGGTAGGAGAACTTGAATCTTGCATAGATTTAGCGGAGCATGAAGCTAGAAGAAAcTACCCAGATGAAGTAGAGCAGAAATCTGAAAACAATGAAAACAGAAGGATTGGAAATAGCAATAAGCCTTGGATAAACAAGAGGAAGGCCTGTGATATTGATGAAACAGACCCTGAGCTCAGCAGGGTTGTTCCTAAAGATGGGCCACTGTTTGATATGAAAGTAAaGATAAAAGAGCAGGAAGTCCTGATTGAGATGAGATGCCCTTCcAGGGAaTATTTGTtGCTTGATATCATTGAAGCTCTGAaCAaTCTcAACTTAGACGCTCACTCTGTTCAGTCaTCAaCCaTTGACGGCATTCtCACTGTAACCCTTcAGTCTAAGGTCTACTTCTTTCCTAaATtGTTGCaTATTTAGGtCTAAATTTGTTGAAGATATAATTAATAAAGTTGCCCcTCTCTCTAACAGCTTAAGCTTTTAGATAAGTTGGTGGTTAACAATTCAATaTGGTATCAGAGCAGGCAATAGATCCTGTgTTCGAATCTCACTGCCtaCCTGATAATTAAATACTTGCGTGTGTTTAGCCCGCTTATGGGGgAgAGCATGGCTACACGTgAGGGGTCgTGTTGAaGATATAAATAATGAAGTTGTCCCTCTCTTTAACAAcTTAAGCTTTTAGATGAGTTGGTGGTTAACAATTCAATAAAATTTTAGAGACATTAATCACTTTCCTATTTCATAGTTTTGGAAAAAaTGTACTTATACTAGAAATTTTGTGCTACATATGCACTTTCATCGTTTCTGAGATGCTAGTGATTCTTTAAAAACTTGATTTCAGTTTCGAGGAGCAGCAGTTGCATCAGCACGGATGATCAAACAAGCGCTTTCAACTGTTGCCGGAAAGTGTTGATAAACTCAAGTTTGCATATTAATGACTTCTGTCTTAGGATTTGCTTCAAAACGGTGTTGTGTAATATGTATCATTGCAGTCCTTTCAGTCTTTGAGTTTTCTGGTCTCTTAACAATCACTTCTCATGGTTTACTGTCTAACCTAGGAGCATAAATGTGGATGTAATTTGGCTACTATCTGCACCATGTTTCAGTTTCATGTCAATGAACTACTTCGATTTTCAGCCTTGCTAGTATTTCAATCTACTCTGGCTGATGCAATAGGAGTTCTTTGGCACATGAAGAAAGGGAAATGGTTAAGGAGAGCTAAATCTATTATGATTGTAGTTTTACAATTATGACTTCAGTTCAAACAGTTACTGAGTCTCCATTAAATTGAGATGGTAATTAACACCCTGGGGATGATGCAAGCTGGTAGTCACCTGCCAACCATGGGTCTCAGGTAGTCATGGGTTTGACGTCCCCAAGTACTAGACGGCGAAGTTAGTTCTTCAAATTAGTCGGCCCACTGAGCCGCATATCGAGTTATAAAACAAATTGAGATGGTAATTTGAGTTGATAACCAATGCCTAAATCTGTCCTCAAGATTCTGAGCATTAAGACGCAACTTTGTACTGTTAGCAAGATTTGGCATGCATTAGGAGTTAAACATTCAAGTTGCTTTCCACACCCCCTACCTCTTTCTGAATTAACTATTTGATCCATCATTTACTAACTATTTTAACCTTTGGGAGTTCTTGAAATTAATACCCGAGTTTCCACTATTGACTAAACGGATTTTCGGTATTTGCTTCAAAAaAaTTCACTGTACAAGACAAGTCATTTCTCCTAACTAAATGGACTAAATGGTGTTTGTAGTGGGAACGAGTCGTCTTTTGTCATCAGGCTGAAGATAAAAAATATATATATATAGCGTGAACAAATCACCCTTAGTTGGGAAATCTTTAAGCTGAAAAAaAACTGCATCATGCATAACAGGTGCAAACAGCTTATTGTGATTTCTACTAATTGTTCAAGCCATTATAGTGTCTTCATAAAAGGGCACCAACTTTTCATCTTGTCCACCAAAGCTATTAGCATGTGATATGTGCTTAAAGTTCCACAGCATAGCTGTGTTTGGCTAATCCCTTGCAGGGACATGTTTTCATGCATTTTACACAGATTAAAGTGGGCAACGTACCTTATTAGGGACTAAAAAAaGAAAaTGTTGCTACCTATATATAGTAGATTAAATCAAAAGTGGGAACATACCTTATTAGACATGATTATGATGGAATTTGGAATTGCACTGTACCAAAGTTTTGTTTCCAACTACTACCAACCGGTCAAAGCCTCCCAGTCTCAATTGCTGCATTTTtCAAATTACTtGTTTtCCCCATCAATTACCCACATACATATAACTCACCACATATTCACTCAATTGTGTTTGGATCATGAATTtCCGGCAAAATATGAAAAGCGAAGAAAATAACGAAGTGAATAAGCTAAATTTATTTCGTCAACTACCACTATTATACCTTTCGTAGATCCATAATCCAAACACAGCCAAAGTATGACAACCCAGTGATTCCAAGAAACCACAAAAGAGTCTTATTCTACAACAGATGATTGATAAGAACTTCTTCTACCATGAACAATGGCTTCTATTTACATCCACTACATTTGAAATCTAATACCAGCAAAATCTCTgACAGTAAAGCCAATTTTCTCAAACTTTCCCTTCTCATTGCTCTCTCTGAATCTCAAATAATCCGAGCAAACGAATGGCTTGTAAATCCTCGAATTTCCCTCTCCTACCACTTCATCAGGTGCCAAGATCACCTTATCATTCTCAAAGCACCAAAAGAAAGCAAGCGAAAACCGGTTCAGTGGTTTTTtCAAAACAACTCTGTGTTCTGATGACCTTAACTTCTCATTGCTCCAAGCTTGAAGCAAATCACCAATATTGACcACAAGGGTCCCCTCACATGGACTAaTGTCAATCCAATGGCCTTGATTTGATCTCACCTGGAGCCCACCCATTTCATCTTGATACAAAATTGTTAGACAACTCATATCAGTATGCATCCCAAGACCCTCAACCTCTTCTTCCAAACTCTTTGGAGCTGAGTAGTTAATTATCCTCAAATAACCATGGCAGTTTTTGTACTCAGAATCATAATACTTTCTTTCAACATCATCACCCAAGGTCATCAACACAATCTTCACAATTTTCTTCGATAAATCTGTCATTGCGCTTCCATATtGTCGCAATATTTCACTAACCAAAAGAACAAAAAAaGGAAGAAAGAAAACTGTCAACGAAATTTGAACGGAAACAGATGAAGGGATGTTAGGTTGGACTTTTCAATTTATGCACAAAGTGTCTGCTCTTTGAAACTTTTACCACTGAAATAGCAGCCCCTTGTACCCTTCAAATATTGAGGGACTCAAGGGACGTTAGGTTGGACTTTTCAGAAGCTAAGAAATATAATTGACGAGCTGAAAAAACTTCGCTGAAGACGAAAAGATTCTGAACAATTTTGTACTTAAACATCCCCAAAGGAGTTTTAGCCAGATAAGGTGAGTACGTAGATAGACAATAGCATTACCTGAACTCAGAATAGTGCTTGTCAAAGAGAACATCTGCTGAACTCTGAGCAGAGCCGGTGAAGTCTGGTCCAGAAACTCGGAGGCTCTCAAAAAAaGGAGATGCTATGTAATGAGGAGTGTAGGTTTTGATAGACGAGAAAGGACCGAGTTTGATTTtCGTCTCATCAGGAAGACTGAAGAGGAATTCTGAAAGTAAGCGGATTTTATCATAAAGGTCTTTCGAAATCCCATGATTGGTGATGTGGAAGAAACCCAACTCTTTGCAGGCTTCAGAAaGAGAGGACAAAGAACTAGAACATGGCTGCAATGGCTGAGAGATGTCTAaTATGGGtAGTTGTATGCacaCCTTGGACACCATTTTctATATACAGGAAAGGCTTGGTTTgTCCAtcTAGGTTGGGGCTATTATATATAAATCAACTGATAAAACAACTTTtGACACTACCATAATATtATtATACAGAGGCCAGAGTAtGGTAGAAaTAGAGATTCACTGTATagCATAGTTGCTTAGGAAGTCTTtGTAAaCAAACTTTCATGAGACTTGTGAGATTAAACATTAAAGGCGTAATTATCTTCGCTTGCCATAAAATGGTATAATAGTAGCCAATTTTTCGCCATTTGTACCTCAACCGTTCcTACTTTCGTTTtCAACTAGCGTACATTCAAAGAGGAAAGAAGAGGGGAAAGGCATCCCATTATTGGTGATGTGGAAGAAACCCAATTCTTTGCAGGCTTCAGAAAGAGAGGACAAAGAACTAGAAGATGGTTACAATGGCTGAGAGATGTCTATTATGGGTTAgTTGTATGCATGCCTTGGACAACATTTtCCTATGTACaGAAAACGCTTGGTTtGTCCACCTAGGTTtGGgCCATCATATATCCTAtATGGCTATTCTAGTCTTTtGAAAATGAAAGAGagaTATATGAaTGTcTATGAATCAACTGTCCATTTAACCCTcTTTTTtAGAACCctATGATCATTCAAATACtGTCATCAAGCATGTACAGTTCagTAACTCCCAAACTACCATTAAAACTATAAGGACCTTTAGGTAGTGTAACCGACAAGGATTGAGCTCTCTTTCGATGCTCTCAGATTGATCTAGTgaGGAATAGAATTGAGTAtGATGTACATTTCATATGTGCTATcGATATCCTCATCATATATGCAAAAAATACTGtATGTACGACGATAATGTTGtACGTTGATGTCTATtGAGCTTTTACCaTCATAAGAGTAcAATTTTACATGAAATATCCTGTGATAAACAACTTTTGACAGTACCATAATATTATTAATAGAGGtCAGAGTATGGTAGAATAGGGATTCACCGTATAGCATAGTTGCTTAGGAATTCTTTGCAAACAAAACTTTCATAAGAcTtGTGAGACTGAACATCAAAGGCGTAATTGTCTTCACGTGCGATAACAAGGGTATAAAAGTaGCCAAaTTTtCGCTGTTTGTACCtcAACCTTTTCTACTTtCCTTTtCAACTGGTgtACATTCAAAGCGGAAAAAAGaGAGGAAAGGCATCCAATGCAAAATaCCCGAAGTTATCaTTTTTCAagCAATGCACAGctCTAAACTGAAGTATTAACTTTGCCTAGTCCATGTCAAAACATGAACCAACCCAACACCCCTtATtcGGTGCAGCTTAGGTAATTATTGAGCCCAGAAGAAAGCTAaTGGACGTTAGAGTCCCCCACAAAAAGGCAGATAAGGGGGGAAAAAGAAAACAACATTGGAGACTAAaCAAATCTCAATGGGTAACACTCTTATTAGACAAAACTGCTGTAAAAAGTTCATTAATGAGTTCTGGAACATTtACACACCAACGCCACAACAACCCGAATTACCCGGTTTTCTAATGGAAACAGACAGAGTAATGATTTGATGCATTTTTtGAtGAAAAaTtCTACAATCAGCCCAATGGGCTATCGTTAAGGTTTTAAAAGTGGGGACTTTTCCGCGTAAGAATCTTCAAAAAGTGGGAACTCTTGCACGCAAGAGTTAACTCTTTCATGCAAAGGTCTTACCTTATTGTCAATCTAGTGGGGACAGAAGGATTGTTTTTTTtATTtGCGGCCACTACACGTTACTCGGCCAAATaTATAGGAAAATATGACCTGCTCCTTTTCTAAATGCAGGGCACATTTTTtGTTCTGAAAAGGAATCTGcGGAGGTAGGTGATTTGGCTCcCATATAACTGTGTAGGAAAGGTGGTTGACAGGTTGAGTAGTTtGTATCTTTCTTCCGGTATTGAGTACCGACTCAACACGGGAAAACTTAAGAcgTCTAGACATAGTAACCATTGACAAACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGACCGTTGGTgTtGTTGGTTCGAAAAaCCGCACCCCCTTAAATCTTGGAAGgAGTTCAGTGAATCATATtGTTAGCCGTTTTGGAAGAGTGTTTGAAGcGTTGGAAATTTACGAAATTTCGGGTGAATAAAATCGGGACATTTGGAGGGTCTCACGAGAAAACTGAAAAATCCTCTCCAGATTCCTTGAATCTGGACAAGGCAAAAGTTATGGGGCTGGTCCCATGATGTGTGGTAACGTCTCAAAATTATTTCAGCGGTTTCAAAATTAATTCTCACCTAGCAACTCAAGGCTTTCTCCTTGTGTATATTATGGAATCCCGTTTCTATAGCCTGGCCCAGGACCATAAATTCGAATTCCATAAGACATATTTTGTTTTTCGTTCATTATGTATTTATGTGAGAGACACACGCAAGTTTAGTTCGCCACGGCTGTTTTCATTGGTGCACTGTGATTTTAGCCGTTTCATTCTGGGATGTAGACTTGTTGGGTATGCAGACAACTTTCAACAATAGCCCGGGGTGAAGGAGGCAACGCCACCACGGCAGTTGGCACAAAAGTTTGTGCCGCAATCCATGTGCAACCCTAAGAGTAcACGTATTGCGGCGAAAACGGTATTTTGTAATTGGGCTAGAGCAATTTCGGCCACCTATTGCAATTGCTTTGTAATTAGGATTCGAGTGTTTTCAGATTAAACACGAGATGAgAaGAGAACTTTTTtGAGAGAGAAGAGAGGACTTGTAGAGTTATACTCCATATTAGAACCCcAAAACGGGTAAGGGTTTGGGTAAAGAGTAAAGATTGATTTTCGATAATTTtACCCAAATTTtGAGTTTGGATGTTAAAATGGGTCAAAATTGGAGATGCTTTTAGAGATACTGGATTTTGATTTTTAAATTTATCACTTGTTGTTTCTCCACAAGGAATTGATTTTGCACTCAATTTTTTTttATAGATGCaCTCCAAAACTTGTCTTTTATTATTtCAAaTTACATGTAACAAATTTtCAAAGCACACTTAAAacAaGTGTACTAGTTCTTTTGTTcGTTTAAAaGGgAAAAAAAAAaTaCTACAGTAATATTCAATGGAATaTTAGTCAATTCGGTTTCCCACCGGTGCAAACCAGTATTTGAGCCGGAACCTACTGCTAAACACGCTTGAGAGATCAACGACGTCGGGTGGGTTAGGGAAAAAAAAAaCCTCCAAtACGTCGCAGAAGGGACCTTGTTTGAATTACGGCGGGACTTGCAACTCTCTCTCTCTCTCATGAGCTTTCTCTGTCGTCTATCGTTTTCCTCTATATATACATAGATGTGCCCTCTTGTTCGGAAAGCATACAACCTGTTCGAAGAAATAgCCCAAAGGTGCCCTTTTGTGCGAAACTCTGTACGTTGTTTATCAAACTGTGCGTTGAGTCAAACCCATTCCTATGAAGACCCAATTCAATTTTCTTATGAAACTCAAAATGTAATATCTTGGACCTCAAAAATCTCCGCTTTAGTGAGGAGAGAGAAGCCCCGAGAGGCCATTGGGTTGTTCAAAACCATGCTTGTGAAAGAGCAAAGGCCAAATTATGTGACAATTCTGAGCATCATACGCGCTGTTGGCAGTTTGGGTTCTGAAAATATGACCCAGGCAATTCATGGGTTGTTGTTCAAAATGGGGTTTGAATCAGAAATTCCAGTCGTAACCGCCCTTTTGGGTGTTTACTCTATGTGGGATGTTGAAGTTGTATGGAAGCTGTTTGATCAAATACCCAATAGGGATATGGTTTtATGGAATGCAATGGTTTCGGTGTGCGCgAaGAGCGGGCAATACAGGGAGGCGTTTGAGATTTTTAGAGAAATGCAGTACGATGGTGTTAAACCAAAAGATGCCAGCATTGTAAGCATACTGCCTGCTTGTGCTGCTCTTGCTAATTTGTCCTTGGGCAGACAAATACATGGTTTCTCTGTAAGAAGTTTATGTTGTTCACTTACAAATGTTCAAAACTCACTTGTAGATATGTATGCGAAATGTGGAAATCTTGAGACCTCTATTCAAATATTCAATAGAATTGAAAAGAAGGATCTTGTTTCTTGGAGGACCATGCTTCATGGGTGTATCGCCAATGATTGCCCTCGAACGGCACTGAATATGGTCTCTAGAATGCAATCTAATTGCTTTAGACCAGATGAACTTTTGATCCTTGATGCAATTGGAGTCTCTACACAAGAAGAAAAACTTAGTTTTGGGCTGGGATTTCATGGTTATATCGTAAAAaGTGGTTTCTTGGATTTTGTCTCGGTTGTGACTGCACTTCTCCAAaTGTATGCTAGGTTTGGTGAATTTGGTTCAGCTGGGGTTTTATTTAATCATATCACTCAGAAAGATGTTATTGCGTGGAGTTCCATGgTATCGGCATATGCGCAGGGTGGACATCCTTtGAATGCTTTAGAAACTTTCAAAAAGATGCAATCAACAGATGAGAAGCCGAATGAGATCACTCTAGTCAGTGTATTACAAGCTTGCTCTtCTTTGGGAGTCCAAGAACTTGCGGAGAGTATCCATGCGCATGTGACTAAAGCTGGCTACTCTTCCAACACAATATTACTACCAGCATTAATTGATATATATTGCAAATTCGGAAGGATAAGGCAAGGAAAGGCACTTTTTGATGAAATTCTTATCAAAGATCTTGTCTGTTGGAGCTCAATGATCAATGGTTATGCGATAAATGGGTGCGGAATTGAGGCTCTGGAGACCTTTTCAGAAATGTTGAAGTTTGGAATAAAGCCCAATGAAGTTGTCTTCATTTCTCTTCTATCTGCTTGCAGTCATTGTGGGCTAGAAAATGAAGGCTGGACATGGTTTTATGCAATGGAGAAGGTATATGGTATTACTCCAAAACTTGCACActATGCTTGCATGGTGGATCTGCTTAGTCGTCAAGGACATgTAGAAGAAGCCCTTGAATTTGTGAAAAGTATGCCTATTGAGCCTGATAAAaGAATTTGGGGTGCTTTACTTGCTGGTTGTAGATCAATTCATGGCtCTATTGAGATAGCAGAATTTGCGGTGGAACGGCTCATTGATTTGGACCCGGAAAaTACTAGCTGTTACGTGGTTTTGTCAAATTTGTATGCAGAGCAGTGTAGATGGGAAGACGTGGAGAGACTGCGGGAACTGGTAGACAATAAGGGATTGAGGAAGGAAATAGGATATAGCATTGTTGAAGCCAATTCATAGCATGCATATTGTGAGATTGGTTAGCTGTGTTCTCTCAACAGGTTTCTTCTTGTTTTTtGTTtGACTATCTGTAGAATGCTGAAAATAAGTTGCTGGATATATCTCTTACTCAGAGATCATACATCCATTTTCAATCTCACCTTTTGGTTTGTCCGTACATCACACTACATACCTTAGACAGAACTTCTTTGAATTTTCCAGTAACGTGAATTTAAATAGGCGTCTGTGAATTTAAATAGCcaTCTATGTATTTATTGTTGCGGAGTTACATTTAGTAGCGGCTTAAGTAAAGAATTTATTGCTCATTGAAAGCAGTACTGTATGCTTCCTTTTATATCCTCCATTAATGCGTATTTCACTAAGTTAACTTTCCAGACCCTTGTTACTAGTCCAATAAATTCTCGAGATCTTTTCCATATAAGCTTTGATTTATATCCCGTTCTGATGAAAGAAGGAATCCTTGAAGTACTTCTTGTCAGCGCTAAAGACATTAGAAACACCAATGTTATCGGTACTCTGTCTCTCTCgCAGCTAATGTCTAAagAGGCCATGTGTATATAATATTTACACGGATTATAGAAAGCACTTTGTTTCTTTGTGTGGCATTTTTTGCTTTCTCTTTTGCATTAATTGGAGCTTGTTAATTGTTCGAACTCAACCTCGTGTTTAACAGGGAGACCAGCATATTATGTGCTCATTGAATGTGGAACTCAAGTGGAAAGAACCAAAACTACAACAGGTCAAATTCTTGGTGATAGTTCATCCATCTTCCTAGCTCTCATCCTAATATGCTTTCCCTAACCTAATTACAGAGTACATTGGTGCAGGTAATCACAAAGAAATATGCTGGAATGAAAAGTTTGCGTTTGAATTTCCCTCTTTCGAATGGGAAAACTTGACTCACCTCAAATTTAAAGTCATGGCTGAGGAATATTTGTTTGAAGGAGGATTTGTTGGTGAaaCCATGTAAGCAGTTTTAACTCAAAACATTATTCTTGTTCAGTTTTCTTTGTTAATTACCAGCCATATAATGCTTTCATTTTCTTTGTTAAACAAACAATTTTGTACTTTCTGCTAGGaTAAATaTCaCAAATCTTCTAGAGCTTCTTTTAtGTcAAACCCaGCAAGGAACTTGCTCTCTCAAACCATCTTCTACCCTAGTAGTATTGAATATTGATCACACGAAGTTAAGAAAAAaGGGTTTAATTTCTGCATtAATTTTTtGTTTTTtGAAATCTTGTGTATGCATTAAGACATTTACTTTGGCATTGGAAACCTTAtGAGTTATGAAGGAATGAATATGTCTTAGAGGAACCGTTGTCTCTCCCTCAATTTCTGTATTTCTATAGGTTAAACTCCTCGTGATGATTATGAACAATGTGATTTGAAGCTTTGAGAGCTTTTAACTAGTAAAGCAGTTCATGAAATAATGGAAGTACTAGCTATAATATCTCcTCATTTGCGTATACATACATTTTGTTTTtGTTGAAATAATTTCTGTATCCCAGATTCtcGGGgCAAATTAACTATCTAGATATTG


>CF810540 Length = 633 

Score = 69.9 bits (35), Expect = 4e-11
Identities = 66/75 (88%), Gaps = 1/75 (1%)
Strand = Plus / Plus


Query: 5863 tgttgaagatataattaataaagttgcccctctctctaacagcttaagcttttagataag 5922 

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Sbjct: 392 tgttaaagatataaataataaagttatccctttatctaacag-ttaagcttttagatgag 450


Query: 5923 ttggtggttaacaat 5937 

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Sbjct: 451 ttgatggttaacaat 465


11B) MYCA1 scaffold 00554 Submitted from SSR primer area

GAAAACTACtAaGAAACATTGTCACAAATGCAGAGGAACACAAACTcTTTCCAAACCAAATGAAGAAACTGAGTTACGTACGCCCAATGTAAACATTATGACTTTTTACtaCTAACCCTAGTGCAAATTATTGCACTATTGTTGCATTATGAAATTCTAAACTATCACAAGAATAATGAAGGGGTTTtATAGGCTAACCcTAacAACCTTAGCAAAATCCAAATAACCCAAAAGGACTAAAATACCCTGAACGTAAAAcaaaaaTAACGATAACCTTACAGAAACCcTAATGACACAAAAAGACTAAACTACCAtGAACATAAACAATAATCTGAAACCCTAAAttACACTCCCCCCCCcTCCTCcTtCAAACGCAGGATGCGAAGCCGGAGAGCATTGTGAGTTTGCCAAGGGATTAACAACAGCGAATCAACTGCTGTGATGGTGCTGAACAATGCAAAGCaGTACAAAACTAGAATtTGCGAATAGGAAGAAGAACTGATGCCGAATAGATGCAAATAGGGGAAACCAATGCCAAACAGATACCAACCAGATGTCAAATTGCtGATGCAAACAATACCAAGAGAGAGAAGAGAACAGAAgCAAAGTGTCAGATCAATTAAGATGCAAAGATCGACAGAGgTGTAGGGAGGAAGAACAGATGCAgAGAGATGCAAAAACTGATGCAGATTTAAGAGGTTCAAAAGTTTAGAGGTTCAAAGGTCCAACATAGCAGAATATTGCAGAATCAGAAGCCCAAGTAAGAGCGCAAAATACCATAGTGGTTGTAGAAGcGGAGGTCGATATAATGTGCACCAGTCAATCGAAAGAAACCCACTCAATTGATGCAATCTGTCGATTCCAAGTGCATTAGGGAgaGACCCAGTCAACGTCGTGGACCAACCCAATCAGTTTAGAAGTCAATCAGAAgTtACCCAGTAAATTTAGGTAGAACTACCCAAAAGATTATGAACATGATCTGAAGTCATCGTGGCCaGaCTGGAGATTGGTTCGCCCAAGGTCGTCGGAGCAAGTCAcTGGGTGGgAGGTGGCTGCTGCTGCTATTGTGTTGTTGTAcGTGATGTTGCTGCTGGTGCGATGAACAGTTCATgTGAATGCCCCGcGAATGGTGGCAAGCCTTAGGcTgCCcGCCGGCGAGtGAATATCCGACAtgCCTAGATCTTATTGCTCTGATACCATGTGACTTTGCAAAGAaGGAAAACAAAACTAACGTATTATtGCaTTATGAAAGTCTAAACTATTACAAGAATAATGAAGGGTTTtATAGTCTAACCcTAAAAaCCTTAGCAAAaCccTAATagCCCAAAaGAAAaTACCTGGACATAAAAGATAAATAAcGATAATCTTActGAAACCCTAATGACACAAAAAGACTAAACTAcAATGAAAATAAACAATAAACAAAAAGACTAAATCAAAGGTTGGTAACTTTGCATGTAAGGGACAACCCACAACTCTACTAAAAAaGGGGGACAAACCATAGATTTCTTTTCAATAACAGCTTtCTTGGAGAGAGAGAGAGAGAaGCATACcTGTAATAAtCCTTTTAGGTCCAAGCTCCCTCACAAAATCAAGTAGTCTAGACAATGCATCAGGAGTACGAGCATAATCCAcAATCACCCCAAATGCTTGTCCCTCATCTATCAACTCACACCTACCAGGAACTGCATCAACCTCTtCAATCCcTCTAACAATATCCTCCAAAGGTGCTCCAACCGCAATTCCAACTGCAaCAGCTGCAAGAATGTTGTATACATTATGTCTGCCAAGCAACCCCGAAGAAATCTCCAATAAACCGTCGGGCGTTTTTACCAAGACCTGGGTCTCAAATAGAGATAGTTCAAATTTCAAAGAATGAACGTCAGCatCTTTATTCTCCACTGCATACGTCACAACAGGCACATCTGGGTTgCCTTGTGCAATAAAGGAaGCTGCATTCGGATCATCAAaGTTGACGATTTTCCTGTGTCTTTctGGGTCCACCATTCTTGAAAACaGCTTGACCTTAGCATCCCTATaCTCTTCCTCagaCCcTTCAAAaTCGTAACGgtCCCtGTCAAATTTGTGAAAACGgCAATGTCAAAGTCAACCTCATTGCACCTCCCTAATGCTAGTCCATGTGACGAAGCCTCCATCACAACTGCTTCTGTCCCATTATGCACCATTTTCGCCagCAACTTCTGAACCAAAACAGCATCTGGAGtcGTACTTGGCGACTGCAACTTaTTATCTCCATGAATATAATACGCCACGGAGCTGAACATCCCAGTTCTCAATCCCATGGCTTCATACAACCCTTTAATCAAATACGCGGTGGTAGTCTTCCCATTTGTACCAGTAATCCCTATCACGGACATTTTCGTAgAGGGGTAaCCAAAAAAAGCTGCCGCCAATGCTGGgAGCACCGAACTTGTGTCTTCCACAATAACCACAGCCTTACAACCTAATGTCTCCTCTAAATCTATCTCTTTACTAGTTACCACAGCAACTGCACCTCTCTTGTATGCCTCCGTCAGATACAAATGCCCATCAGTTTCCCTtCcGACACAACACACAAaCAAATCACCAGCCTCAACAAACCTCGAATCATGTTGAATCCCACTAATCTCTACCTCTAAATTACCATAAACTGAAACAGGTACTACTTTACTCTCATCTAAAAGCTCAGCTAATGTCATATAAAACGTGGGGTCAACAATCCTAAACTTGTGACTTTTATCAATCTTATCGAAATCAATATCGAACGATGAATCAGAACTCAAATCAGACTTaCCCAAATCAGAACTCACCTCATTCTCCAATTTCTCACCCTCTTCATCATCACCCGCATCTGCCGGAATCACAGTGAGCCCTCGCAGCTCCTCAATTTCCTCCAAATCAACCACTTCTTCAGGCTCCAGCTCATCAATACCCTCATCCACTTCCTCAACCTTCTTCTCTTTCCTAGGGTTAGGTTTCAACAAaCCCTTTTCAACAAACTCTTTCCTTGACAAAGCAATAGCCTTATCAATATCAGCAAACACATCATCCCCCGAATCTGTTGGAAAAATATTTTAGAATTTCTGTAAACcAAAAAAaCCAGGGACAACAaTACAActATAGCTACCATAAACATTTGTGGCTACGGGAGTTACATTTGAAATTTGTAACTCTTTTACAACTTTCAATTAGCAAATCATTACAATTAAGTACAATTACAAAGCTATCACTGTTGTAAAATCAAATAATAGGTACCATGAAAATAAGAGAGAGTTCACTCTTGCCTTGGGcgAGGATAACTTTatCCACATTCGGCGGGgTTTTGTGGGTTCCGATTCGGTAATCAGAACCATTcATTTCGTATGACTTGATTTATCCACATTCGgCGGGGTTTTgTGGGTTCcGATTCgTTAATCAGAACCATTTATTTCGTATTACTTGATTTATTAGGAGACcaTGTGAAAaTTCAGCTTAATCATAtAGTTGTAAGCACTCAaTCCAAAATTttAaTTTTtCTCCATCCagTTCTAAAGCTAaGGAATTaGACTTtCTATGCTCAaTATctAATTGATCTAAATTTTTTTGCGTTGTCTCCTAACAAATCAATTCATCCAAAaTAAGCAGTTCCAATTCCCATGGGTAAAATTACACTAGCTCTGTTGAACAGAATTGCTCGCTACAAACAATCAAATAAAATTCAAATCACAAAAAATCTAACAGAATCACCACCTCGGTCTTCGAACCCGGGATCGTCGGGTGCGTCCTCCTCGTCGGCAATTGCATTGAGAAAAATTGATTGCTCCTTTTCAGACCGCTCTTCTTGGATCTTTCTTGCTTTATCAGCATGGCGCTTGACTTGCTGGAACCTGGATACCCCGTGCATGGAGTCTTCTTCCGCTTCGGGCGGGTCATCGTCGGATGGGTCCGGTTGGAATACGCCGTcGGGgCCGATTGCGGCAGGGGGTCGGAGTTGGAGTTGGGGTTTtAGGgTTtGGTGAGgAaGAGaGAGGAaaGGgAaGGgTTtGAaTtGGGgAAAAaaTGTtGGATTTTtCAGAGAGGGGGTGAAGAGTGGgAAGGAGATgAAGGTGGGTGGCAATGGCATTGTAGAGAGAGATTAGAGAGAGAGAGAGAgNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAACTCAATTCTTGAGGCCGCAAACaCAATTCATGAGGCCTCGAACTCAGACTTTGTGGCCGAAaTGCTTTGGCTAAAAAAaaaCATTTTtttCTTtGGTCTTCATTAAAATAAAAaTTTGCATtcGTTAGTTTTGCGACGATTTTTGTGGTCTATTGGTTCGTtCcGACGAGATGAATCTAAAgAGtaTAAAAttAAAATTTTtaaagaTTTCACTAAAGACAAAAaTAATCTAGATTTTTgTgATTtATTAGATTgTTTTtGTCTTTATTCaCAAAAATTTAAGTTtCGTTCATAATTTTAtACTTTTTtAGATTCCTCTCGtGgAGAGAATCCAATAAATCACAAAAAAaTCGATGCAAAACTAGCAAAAACAAATTtCTTTTGAaTTAAAAAAAAaCATTTtAGTTTTTTtAGCCAAAGCAAGTGAACGAAACTTGAATCTTGTGACCTGAAACTcccACCTTGTGACCACTAACTCGAACCTTTTGGCCTCAAACTCCAACCCTTtGGGCCGACAAAATGTtATTtCTCATCTTAAAAActtAGGCCTaTTTTGGCCAAAGTAGAAAATCAAGCTTTtCcTTTtAgCTTTTTTTTTTttGGgAAAAAAaCTCAAAGGTAAAaGGTGGATtGTTTtGGCcTTTTTTttGTGCTTTTTGCTTTTCACCTTTCAAATTGTAATtGTTTTTTtATtACTTTTtGCTTATtGTAAaGTTACTTCTTTAAGCCAAATTCTATGACTTTtCgAaTCtCAAAAAAGCAAAAGGCTTGCTTTTCTAGTTTGGCCAAAATAAGCcTTtATCTCCATAAtttgttGACTACTTTGTtATTCATGATTTTTAaTCTTTAGtgATTTCTGTCAAATATTTtATTTTTTCCCTTTTTAGATGCATTCATACCTCTgTTTtAAAAAATATAAAAAaaaTTATGATATTAAGAAAAAaGACTAAAAaCgAAAAaTAGGCAACTATATACAAGCCTTTTtAAtCtAaaTAAAAAaCTCATCCAAaTAAATGCTACTCTAACTTGTTTTtCTCAACACTTTTGTCAACAACGTTTAAaTAATCTTGTGAAATGGAAATGAAAACTATTTTAACGCTCCTTTTTTTCTTTCTATCTATTGgCACTCAATTTCTTATCTTTTATtgCGTGACAAAaCTAAACTATCCTTGgTtCTTATCTCTTGAGTCAATTTTTtCATATAAAAGGTAaTTCGAtACTtCCGCATGAATAAAAACCAAAAAaTGGGTGCCAATGGATAAAAAaTTaGCGTTAAAatCATTTCCCAAAATAAAaaTaGTTgCGTCAAGGTTCTAAAtaCCATAtCGTTTTTTCtAGTTGTACAATATGAACCAGtNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGCACTATGCTGCACCCTCCTCagATATTTTTGGTCagCTTCCCGCCAAACCCACTAGgCTAAAACCCTAGTTGCCTTTTCCCCCACTCCAaAACCCTAGTTCtCTCTCTCTCCCTCCGCCACCAGTTCCCCACCCcACCACAACCAACCATGGACCCTCAGTCcGTTCAGACAAACCTAGTCCTCATCCTAGACTTCGGTtCTCAGTACACCCACCTCATCACgCGACGCATCCGATCCCTCTCCGTCTTCTCCCTCTGCATCTCAGGCACCTCCCCTCTCAAATCCATCACAGACCtAAaCCCAAGTGTCATAATCCTCTCCGGTGGTCCCCACTCGGTtCATgCGCCCAACGCGCCGTGTTTCCCtGATGGGTTCATAGAGTATGTGGAGTCTAAGGGCGTGTTTGTTTtGGGCATATGTTATGGGTTGCAGCTGATTGTGGAGAGGCTGGGTGGGTGTGTtGGGgTtGGCGAGAAGCAGGAGTATGGGAGGATGGAGATTGAGGTGGAGAAGGCTTGTGGTTTGTTTGGTGGGTCAAAGAAAGTTGGTGATAAGCAGGTTGTTTGGATGAGTCACGGGGATGAGGCTGTGAAGTTGCCTGATGGGTTTGAAGTGGTGGCGCGGAGTCAGCAGGGGtcGGTGGCGGCTGTGGAGTGTCCTGGGAGAAGGTTTTATGGCCTTCAGTATCATCCGGAGGTAATTGGGTATCTTCATTCATTTCATTCTTGGCCTTGTGATACGCTGCCCTGTTTTAATTGTCTTTGTAATTTGGTtCTTTGGCTGGCTTTGTTTGGTTCTTTACTAGTGGTTATTGTTTGTACGCGAAGTGCTTACTTGCTGATATCATCTTCATGTTGAAGTGGTAAtAATGAATGAAAAATGGAATGAATTGACCCAATATCTGAAAAGTTATCGTCTTTAGTGGTACTGTGGTAGTGGCCTATAAAACTGTTAGCGAcACTCCCTCGtGATGTATCCTGCTCAGGCCcAGATCCTTAGCAAGAATGAGTCAATGACCATTGATCCCTTTtCAAGCACTTAAGCtgAaCTTCCACATGgTACCATAAAATCCCTGAATCCTCTCAGCACAAGCACTGATGATCTCTCTGTTTGAGTTTCACATCACTAGCCCAACCCCCAGAAAACcATCCTTATAGTTATAGTAGCCCCGTCAAACTGAATTGTGAGCTCACCTTCATTTGGAGCTTTCCATGAGCCAGCAAGATGTTGGGATAGTACTACAGTCTTTGGAAATACAATGGGAGTTCTCTCAGGTAGCTAGCTGCATATTCtGCAAGATCACTGGCTCCCCTGTTTGTCCCtccAAAAAaTGTAATTGCCTATTTAACCAAATTACCCAAGCCACAATTAGAAagAGATCTATTATTtGTTGGAACCAACCCCCTCAATCTGCATAGCCCAGAGATGCCCCTTTCTTCAAGCACATGCAAATCTTTGAATCGATAACCTTCCGTAAcATTCTTTGCTTACTCACaCtCTTTTAGACAACGAATGATGGTCTCCGGTGCGGCGCTGTCCCTCCACACAGGCTACAACAATCCTTAATATTAACCATTTTTTtGACAAGCTTATGGTTCATTGGGAAAATCCCCAAACACAtCTTTCAcacaatgaCACAAACAATTTACTTTgTGTTGGgAaTTTTCAGATTCCAGATTTCTCCTAAGATCTcATATTATCCATGTCAGAAGATGAGAGTTGAGAGTgCTGAAAATGTAATGTCCCTTGTGGTGTAACCAGACtCcATGGTGTAAGTTTCTTGTTTAGTGAAGTTCCATGTTTAGTTGTCTTCATTTCCCAGGaTAATAGGGGAATGTTTCATGtCcTATgAGCTTCAAAAGGGaTAAAaGATCACAGCTAAGATGTTCCTTCCAAACCCCAGTATTGTGGTCAACGAGGCCCTTtACTaCCATTCTCTCCGTATCAGGCTTACAaGAGCTTGTAGTTTTAaGGGAGAAGTGCTTGGgATCCATtGGTCTGGCCACACCCTGGTTTtAtATCCcTTTCcGATCCtCcACCTAAGaCCCTTTTTAAGCACCTCCATACCCTCACACAT

>DR067752 Length = 574 

Score = 46.1 bits (23), Expect = 3e-04
Identities = 29/31 (93%)
Strand = Plus / Minus


Query: 459 gaagaactgatgccgaatagatgcaaatagg 489 

          ||||||| ||||||||| |||||||||||||

Sbjct: 353 gaagaacagatgccgaacagatgcaaatagg 323


12) UFGT search with amino acids using tBLASTn SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL


>CV091309 Length = 663 

Score =  124 bits (310), Expect = 1e-30
Identities = 60/60 (100%), Positives = 60/60 (100%)
Frame = +3

Query: 1 SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL 60 

          SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL

Sbjct: 138 SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL 317


>DR067838 Length = 557 

Score =  119 bits (297), Expect = 4e-29
Identities = 58/60 (96%), Positives = 58/60 (96%)
Frame = +1

Query: 1 SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL 60 

          SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKG  L

Sbjct: 352 SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGTGL 531

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