Malcolm C

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Revision as of 19:30, 12 April 2012 by Macampbell (talk | contribs) (→‎EST Search Results)
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This is where I will capture my progress on this project.

I will also be working on the 13 genes Allan gave us.


SSR Primer Hunt

The class has already completed 4 SSR primer designs (LDOX, CHS, StSy, and F3H).

Results from Blueberry 454 Scaffold Searches from Grape (given to us by Allan Brown)
AOMT Scaffold 00796

  1. Forward Primer GAGCTCTTGGATGCTCCATACT & Rev Primer CCTCATAATTTTTGGTCTTCGC (taa) x6 PCR product = 137 bp
  2. Forward Primer TATCCCATGTTTTGGCTAATCC & Rev Primer TTTGCTTTTCAATCGGGAAC (tc) x9 PCR product = 261 bp


AOMT scaffold 01781

  1. Forward Primer GGCGACTGTGTCTTTCAGTAAA & Rev Primer TAGGATTTCGAGGAGGAGAGGT (ct) x7 PCR product = 136 bp
  2. Forward Primer ATAAACAGGTAAACCGAACCGA & Rev Primer CCGATTAAATTGGAGCAGTAGC (tg) x7 PCR product = 262 bp


ANPER scaffold 00666

  1. Forward Primer TTTCTATCTCTCTCGCCGTCAT & Rev Primer GTTTTCTCACCTTGTTTTTGCC (ct) x21 PCR product = 219 bp
  2. Forward Primer CCGTTTCGATCTCTCTCTCAGT & Rev Primer CATCCCTAGATCTGACACCACA (tc) x14 PCR product = 278 bp


CHI scaffold 03186

  1. Forward Primer TACATCTTGAGGGGCAGTTTTT & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp
  2. Forward Primer ACGTGGTCCAATCTAACCTCAC & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp


DFR scaffold 03132

  1. Forward Primer TACATCTTGAGGGGCAGTTTTT & Rev Primer GTGGAGTGTGGGATATGGATTT (tc) x5 PCR product = 121 bp
  2. Forward Primer ACGTGGTCCAATCTAACCTCAC & Rev Primer TAGTAAGTGGAGGCCGGTAAAA (cttt) x4 PCR product = 288 bp


F3'H scaffold 01438

  1. Forward Primer CAGTGGTACAGTGGTGGATGAC & Rev Primer ATTCCCTTTCTCTTCCCATTTC (ag) x7 PCR product = 152 bp
  2. Forward Primer ATACCAATCCATAGTCTCCCGA & Rev Primer TCAGTTGTGTTTTTCAGTTGGG (ta) x7 PCR product = 279 bp


FLS scaffold 00142

  1. Forward Primer CAATTATACCCTTCCTGTCCCA & Rev Primer GAATGGATGAAAGGAACCCATA (ag) x8 PCR product = 214 bp
  2. Forward Primer CTAGCTCTCCCTCTCTCCCTCT & Rev Primer GTGGTACTCCATATCCATGCCT (ct) x8 PCR product = 295 bp


FLS scaffold 00374

  1. Forward Primer TGAGCATCATTGCCAGAGTAGT & Rev Primer GGATTTCATTCCAAGAGATTGC (ct) x6 PCR product = 295 bp
  2. Forward Primer TAGATTATCCGGGGAATCATTG & Rev Primer GATTCTCATGACAAGCCAGTTG (tc) x6 PCR product = 275 bp


MYBA1 scaffold 03500

  1. Forward Primer GCGAGGATGGAAATATATACGG & Rev Primer GCTTCATAAGCTGCTAGGCAAT (ag) x8 PCR product = 222 bp
  2. Forward Primer GCGAGGATGGAAATATATACGG & Rev Primer GCTTCATAAGCTGCTAGGCAAT (ttat) x5 PCR product = 222 bp


MYCA1 scaffold 00278

  1. Forward Primer CAGTATTTGAGCCGGAACCTAC & Rev Primer CGTACAGAGTTTCGCACAAAAG (ct) x7 PCR product = 268 bp
  2. Forward Primer TAGTGGGAACGAGTCGTCTTTT & Rev Primer AGTTGGTGCCCTTTTATGAAGA (at) x6 PCR product = 195 bp


MYCA1 scaffold 00554

  1. Forward Primer GGCTAAAACCCTAGTTGCCTTT & Rev Primer GGGTGTACTGAGAACCGAAGTC (tc) x6 PCR product = 157 bp
  2. Forward Primer CCCAAAAGAAAATACCTGGACA & Rev Primer GGAGCTTGGACCTAAAAGGATT (ga) x8 PCR product = 260 bp


UFGT scaffold 00304

  1. Forward Primer TGAACTACCAAAATGGGACAGA & Rev Primer NGACGGAGCACATCCTACTACA (ag) x10 PCR product = 207 bp
  2. Forward Primer CCCCAAATTCAGATCAAAGAAG & Rev Primer CTTATCTCTGCCCACCTACACC (ag) x12 PCR product = 230 bp


UFGT scaffold 01120

  1. Forward Primer TGTGTGTGTGTAGTGTGGTCGT & Rev Primer AAGAGAGAGGGGTCCTGAAAAC (ct) x11 PCR product = 132 bp
  2. Forward Primer TAAGCTACAGTACCTCGCCACA & Rev Primer AAGGATATGCCGACGAGTCTTA (tc) x6 PCR product = 196 bp


EST Search Results

For the second half of the semester, we focused on finding ESTs of our genes using the Washington State University Vaccinium genome database as well as the Towson State University Blue Berry Genome database using 454 sequencing.


ANPER - anthocyanin permease

AOMT - anthocyanin O-methyltransferase

CHI - Chalcone isomerase

DFR - dihydroflavonol 4-reductase

F3’H flavonoid 3' hydroxylase

LDOX - leucoanthocyanidin dioxygenase

Myba1 - myb-related transcription factor

MycA1 - myc anthocyanin regulatory protein

UFGT - UDP-glucose:flavonoid 3-O-glucosyltransferase


1) ANPER Submitted the 3’ most blueberry sequence from my scaffold00666
ggatggcaagctctggtagcttacatcaacttgggatcctattacat
Got this result from BLASTn search of Vaccinium database
>DR067294
Length = 260 Score = 60.0 bits (30), Expect = 9e-11 Identities = 39/42 (92%) Strand = Plus / Plus 

DR067294.png


2) AOMT 01781 search Submitted carboxyl most amino acids that gave hits with my scaffold01781 Did tBLASTn EEMVLEDLRPSRESTMKLNKMLAADPRVMISHASIGDGITLCMRV No hits.

I submitted ATCGAGCTAATATTTTATGAGACcACTTAAAAAaTTTAAAAAaTTATTTtAATATTAATGAACGATTCGGCTCATTTGTACTCTACGGA From the scaffold

And got >DW043195 Length = 523 Score = 48.1 bits (24), Expect = 7e-07 Identities = 33/36 (91%) Strand = Plus / Minus Query: 44 ttattttaatattaatgaacgattcggctcatttgt 79 

         ||||||||| ||||||||||| ||||| ||||||||

Sbjct: 44 ttattttaaaattaatgaacggttcgggtcatttgt 9

3) AMOT 00796 search submitted ATCTAAAAATAAATAAATAATTTGAGTAATAATTATTAAAGACGAAAAAaTAAaGATGACGAAATAGTAAAAGCTTTGGAAGATAAATTTTCAAACCTGGAAACCCGCAAATTACTCCCACTTGCATATATAAGTACTCATATAATTTTCTATTATTTAATTTAATGGGTCCTAACATGATTGTAGTTATTATTTTTTTTtATTATTATTGTAGTTATTTATACTACGCAAGATGAAGGGTGCACAAGTCTTCCATGTTGTGGAATATCGCAGCTACAAGCATTTATTTAATGGGTACAAAATATTTTTGTAAGCGTCGtGCCCATTATAGGTGAGCATATAAGTCTATAAAGTATTAATTTAGAGAGGTCAATTAAAAAaGAaTCTCCCTGTGACACTACAATCGTGCTTGATGTCAACAAaTATGaCAAAAAAaTATATaTAAAaTaTAAAacAAgTACTTtCAtgcATtATTtaTTtAAtATTTttCAcACAAATTTGAAATTGATGCCGAAAAATTAGAAATTTCACATAAAATATTTATTTAAaTTAAAAATAGCACGGTTTTTCATAAGGAACTAAAATTACTTGGCGGACGGAATACCAGAAAATCACGCAATTAATTCTTCAaGTTTTTCACTTTTCACGGAGGTGGGTTTATGATCtCGTAAATAAGTAGTTTCTTCTTTTTTtATTGTGGATATAATCTCCTTCTATTTTCTCTAAATATTGAGGATCATGATAAtGCAAAGATGCAAGTGGTGGCTAGCTCATGCGGTAAGAGCTCTTGGATGCTCCATACTGCATCCCGAGATCGAGTCCCATCTCCCTTCCTAATGTAATAAATCAGGAATGATGAATGAAAAaTAATACGGAGTAATAATAATAATAATAATGCGAAGACCAAAAATTATGAGGATTTTTTtAGGAGGGTTtCGAATTACACCATCcTTTTTGGTAGGTGAATGGTTCAGTGCATGTGAGCATTTTAATAAAATCATTACATATTTTGGtATCAAATGGTATTAGTATATATAATTAATTAATTAAGTAATTATGACCCCTTGCATGTGAATGAAGGTGGTGGTGGGACCACCAACACCAATACCAACTCAACGGCATATACATATTGGAATCGTCAATAGcGGGCAAGTGTGGTTGGTGGAaGgTTGGTGAAATGAAACTTTaGtCcACGTAACAaGAAAaGAGTTGGTGGCCATCCCcTTCAAGGGATATCATTTTCTTTTtAAAGGGCATaTAAAAAAAAAaTtAAAaTcACTAACAAGAAATTTTAAGGAAAaTAAaCAaGCCCAACcaTAAAACGGTAgATACCAAaTCTATACCACCTTATAGTATATCTATTTTGgAATTtCACACTAgTGATCAAACGGaTGTGGGgTTgCTGCTATGCAGCCCTAGCACAACAAGTGCTGTGTATTTGGCTTGTTCGTTACTGGGATGGACggCCcAGATTTTACCtCGGCACCTTTGCATCGAatCGTTTGTtGTCGTGCGTGCAAGTGAAATCTGGATCGTCCATCAGAGCAACGGATGACCCGGATGTGCATAGCACCTGCTGTGCACGGGGgTgCACAACAGCAACCCcAGTTCTGATCAAACcAATTCTATTATTTtAAaGATTATTTTATGGgTCTTtGAAaGAAAAAAAaaCTCATTCGGATGTCGTTAAGGGCTTAATAGGACGAAAATCGATCAAGCATTTAAtGATATtTGAATGAGTTGATTTTTTataGagACTCTtAAGAAtaTGTTTTAAATCAATTGAAtGACTCTgaTCATTTTTGTGGAATTCCAAAATAATTTTtACACGATAGGGTAAATATTTGGCaTACACCACATGGTATAaGCAtAGTTTTTTTTTTTttGgtGCCTTTACTTGgTTCATTTCCTTTATTTTTGCGAGTCTAAGTCATTATCGTTTCTAGGGATGGCAACGGGTAAgaCATGGATCAGGGATCCTATTTTACCTTCGATCCTTAAATGGGAATTTCCTTTCCACCCCcACcTCCATATCTAAAAGGGaTAGTgTTttCCAcgATACCCGCCTCCCAATGGGGATCGgaTACACTtGGGTAAATtcATATCCAAACTAGTTGAAAAAaaGAACAaTAAGATCTAAAtAAAAATAaTTtaCgTGTCC


BLASTn Got 3 EST hits

>CF811118 Length = 663 Score = 60.0 bits (30), Expect = 5e-09 Identities = 49/54 (90%), Gaps = 1/54 (1%) Strand = Plus / Minus Query: 755 gcaagtggtggctagctcatgcggtaagagctcttggatgctccatactgcatc 808 

          ||||||||||||||||||||| ||| ||||||||||||||| ||| | ||||||

Sbjct: 104 gcaagtggtggctagctcatgtggt-agagctcttggatgccccacattgcatc 52


>DW043505 Length = 532 Score = 58.0 bits (29), Expect = 2e-08 Identities = 39/41 (95%), Gaps = 1/41 (2%) Strand = Plus / Minus Query: 755 gcaagtggtggctagctcatgcggtaagagctcttggatgc 795 

          ||||||||||||||||||||| ||| |||||||||||||||

Sbjct: 142 gcaagtggtggctagctcatgtggt-agagctcttggatgc 103


>DW043214 Length = 426 Score = 48.1 bits (24), Expect = 2e-05 Identities = 27/28 (96%) Strand = Plus / Plus Query: 754 tgcaagtggtggctagctcatgcggtaa 781 

          |||||||||||||||||||| |||||||

Sbjct: 374 tgcaagtggtggctagctcacgcggtaa 401


4) CHI

I did BLASTn with (from scaffold near SSR primer) TTTCTTGAGGTCTTCCAGGATGAGACATTCCCCCCTGGTGCTTCTATTCTTTTCACCCAGTCACCCCATGGGTTGCTAACGGTCAGTACATCCTTCCACAAGTGTTAGTTCTGCCTTTCTCTTCGCCTTTtGTTCTCTCTTCCTTGTTGATTATAAAATAGGTAATGCAGTTCCTTGATTCAGGAACAATTTAAGCAGGTATCCAAATTTTCTTTtGAAaTTTGAGGgCAAGGAAATCACGATGATTTATGAGACTGATGTTGCATGTTAATTtCAGCTTGTACCCCAGATGCAGCATCGATCAGGACATTGTGAATTTCTTTtGTTTTtGGTCACCATTACTTGGTTCTCTACTAGTTgACGGTTCACTGTGCACTAaGCTCCATTTTGATGGACTTCATAAAACaCGATGCAGGACAagATGTAAACCAGGCCCATGAAAATAGTTtCGTGTTTTAAATCaTTAAGCCTGTTCTTGACTTCGCCAAGAAGAtCAAACTCGTAGTATAAAGTATGTTGTTGAGAAGtGTTCTGAAAAGTAAGcAAATGGGTTTCTATTTtGGTTTTCATCTTTTTATAACACTCTTGGTTAATTTTtACCCTAAAAAaTACTCcATTAaTtAAGACCTAAAGACAAcTGGTTTGGGTtGATTATAGGTTTACTTTTATCTGTTtGGTGACATCACTAGATaGGCTGTGTTGCGACCTTGCCGATTGGTTTtCACAATCGGAGCACCTGAGTTATGAAGAGACATGCTTTTTtGAGGAACTTTAGGCCCGATATCGTCTTAACTCTTGAGAATCATTTTCTTCATTGATTTGGACATACACACACATGCATTCGAATCCGTGGTGCAAATGATTCTGTAAGATACAGGTCATTTGAATCTTCATGCTCAAAACCCCCATTCAATTAGCGACTTACTTCTTACTTCCCAAGGAGCAGGAACTGCTCTTTTAACCCTTCTCCGAGGATTACATCCACAAACACAAAGTCGATGGCAGACTGATATTACTCATCATCATTATCTATTGCATTTATTTTTCTTGTTGCTGATCATATGTATTTGACAAACTTTGGGATTACATGAGTATGTACATCCACAGATATGCATTGGTGTTGCGCGTGTATGTAGGAAGTTGCAATGAAACAATAACGTTTTGATTTCCTATTGTTGATGCTTTATTTTCTGACTACAGATTGGCTTCTCGAAAGATGGTTTGTTACCTGAGACTGGCAAAGTGGTAATCGAGAACAAACAATTGTCCGAAGCAGTGTTGGAATCAATCATCGGGAAGCATGGCGTTTCCCcTGCAGCAAAGCAGAGTTTGGCGGCAAGAGTgtCTGCATTGTTCAAGGAGGCATAAGAAAGAAGGTTTTGGAAAACGATAAGAAAGAAGTCCTGTAGCAGAAAagttAgTAGtAGTTtATTGAgagaGGAAGATAAAATGGTAGAATCTCACTTCCCAGTTGATTCTGATGTTTCTCCATGAGTAAATGTATCTGTACGCTCTTTCATTTCCGTTTtGTCACTGTAATAAGAAGGCTTCAaCTTTTGCATTCTAAACTCATCACATCTATCTACGACCTTTTCTTATTCTTCTTTgCAAATTTAAACCAAACcGagCCTTATGtCCCAATAaaTTGGGgTCTACTATTGGGgTCTACTATGtAAatCCcTTTTTttGtCcATTGGgATCTGtGCAAATTCAAATGATATTGAAATTAAaTTCGGCATAaTGGCGGTAGTGGTTATGGACATCATGAATTAGTAATGTCAAGTGTGGAAATTTTtGCTTGTTTtACTTTGtGTAATGCAAGATGTCGAGGCCAGTATACAAGGGTGGAGCCAAGATTTTAGGTAAgCGGGGtCAAAAATTAAACGCATATGCAATATAtATTTTTTCtATTTGCTTATGCAAAGAAATAACTAATTTGAGAGTTTAACTATAACGTAAATATACAATGATGCATGTATTTCTGCTTTATAATTGGTCCCAATGACTTATTATATCTTGAAAAaAaTCACTTATTCAGATTTTTtACTTATTTAATAAAAAAaGTGAGATTATATtAGCTTCAATTTGTAGTTAATTTATTTTAAAGTACTTGAAAATGTTAAAAAaGGTTTGACCAAAAaGTTTATTTATTTTCAAGAGTGCTTCTATAAATTCCTTAAGACAGGTAATTAATTAGAAGTTATTCTACAACTTATTTAACCAAATAATTTACACTCTTCTTTTAACTTATGAAAGAGCACCAAGCGTATCTATAAATTATTTATAATTTTTAACTTTATACTTCTAAAAaTCATCATTTAAAATTTtAACTTATGAAGGAGCAAACAGCTGGgATTGATTTAAAATCTTCGGGACTAGTAGAGCCGCAAGGCCCGCAAAGACTTGCTTGgTTTTGCATACAGATTGCCCATTTGATTTCCTTTTTATGGGCCTTGTAATTTGGTCTGATTTAATCAATAGAAACTGCGTTTGTTCGTCCTAAAAAAaGGTTagATAAAGATCCTCTTATTTGATGATTTCTATTGTGATGCAAAACGAAATTACATAGCAAGCTCATGTATGTATTGGAGAGTTCAACACTTTATAGCTTTTTACGATAACTCGTATGTCTGGGCTAGTTTCAGTTCACCTCGAGtAATTTTGGGGACcAGTCGACTGTTTACTAGTGAGAGCTCCAATTAAAGCTAAAGAAGCTCCGTATGAATTGACTCAAAaGAGTTGAAAGCACCTAAGATGTTTCGATCCTAAAATCTTAAAAGGAAGCAAACCCCTAAGTTTTAGGGTCATCAGGCCAACTGCTTAGGgTTAACTATTTATAGCTTTTAACTTGGGGAGGTTGTAATGGTTATTTACAATGATTAAAaGAAAAaTTATGCATAACGACAAAtCCAATAATTAAAAGCTTCATAATCCCTTCTCTCTCTAAAACGAAATCCTCTCACTCTAGAAaCCAAAAACCCTTATTTCTGTCTTtCCTTTtCCcTTtCCCCcTAGGgCTTCACCCATGCGGCAACAGTGTAAGAACCcGTGTTTTTAAATGATTTTTCAGAAATAATGTTGGGAAAATGTAAGGAAACgAAAATAGATTAAAGTGAGCCAATGTGGTGTTAATAGATTGCATGTGAGTGCATTTAGAACACATTAGAGACATGTGGCGTGTGTGTGCAATTAAGGGTGTGCATATACATCTTGAGGGGCAGTTTTTTTTTtAAAaCtAAAaCACTTCTCTCTCTCTATAGGgCCcGCTGCCCCcTTTCTCTCTCCcTCTCCATTTCTCTTGCCAAATCCATATCCCACACTCCACCTCTTTAAAATCTAACCTTAAATCCTTCATCTATGCGTGATTTGGAGTCCGAGTGTCTTTGGTTTCACTTGGAGgAGGTATATTTtCGGTTGAAATTCAAGAACAAAAgTTGGGGTTTCATTTCTCACCAATTGGAAGCTAAATCTTTGAGGTAGTGGACAAATTTCTACTCCCTcTAGTTGTATTTTGTTGATTTGTCGCATATACCATTGTGTTGCCCACAAATTtCTAGTCAATCGTGAAATCTCCCTAGAAATGGGTTTGGTTTTGATTGAGTTTGATTCCCACTTGTTTGGTTGTTAAATTCACAAATTGGTGGTTGATATACCCTTATTCCATGCTTAAATGATATTTGAAATTCCAAAATTGACATGGGTTGGAACCCCCCTTCATTTGTGAATtGCTGTTAAAAATCTCATTTTGTAGcAGTAGATACCAAGTTAGTGAGGTACGATACCTTTTGGTTTTGTTCACTGATGGCGCGTTGGTATCTATACCAAGTTGGTGAGATATGGATACCTGTTGGTTCTGTTCATTGACGgTGCACTGGCATCCATACCAATTTGGTGAGGTATAGATACCTTTCTTTCTGGTCGCTAAtGGTGCATTGGTATCGATACCACTCTTGGAGGTATCCAtaCCTTTTTAAAAAaGTTCCAAAAAACTCTCATTTGGTTTCCGGTTGACTTATTTGGTATCATACTTGATATATTCACCCCGTGCACTTTATTTTTGGTCGAATTGCGCCTTTCTAACCTACTTtCATATGATTGGTGATAaTGTTGATCTTCCAACGTACTTTGACACATatAAACTTTGTAGAAATTGGCGATCTACGTTACATGAAATGAAAATGTATGATGTTTGTTATTTGGACATGTGTGAAATACTTGGTGATTTttGAACGAAAAaCAAGTTGATGCACTAGGATCACTACTCAGTGAGTCACTAGGAAAGGTTTCACGAGGCCCATAAGACTCGGACAAGGGTTCTACGAGGCTCATAAGATTCGGATATGAGTTCCACgAGGCGCATAAGACTCGGACATGGGTTAAGACTCGGACAAGGGTTCCACGAGGCCCATAAGACTCGGATAGAGGTTCTACGAGGCCCaTAAAACTCGGACATGGGTTCCACGAgCCCATAAGACTCGGACATGGATTCCACtAGGCCCAAAAGACTCGGAAGAGAATTTCAATATTCTCAAGCTTGAATTGATGTACACAtGAGATTGATAAAATGTGAAAATGGTTGAaCGTGGACATTTATGGGATACGCGGATACTCAGAGACACAGTCGGGTAGTTTACGGgCATGTTGGGTTTTCTGTTATCATATGTGGGTCCTATATGGACATTTGACCATATTTTTACCTTTGGACATTTGTTGGTCATTCGTCACATGTTTCGAATATTtCGTAGTATCCATTGGTTGTATCACACATGATAGTCATTCTCTTATGCACATATCACACTTTATACACCTAACGTTTGTAGAATGGTTTtCCTAATGGGCTAGTGAGCTCACCCTATCTTTTTCAGGTACATGGCAAGAAACATGTGTTGGATGCTTGGAGTGTGGATGCATtGCATGAAGCTTGTTTGATCATGAAGTCATTTTATCCTCCCCATACTTAGTATATTTTTGGGAGATGATCATGTAACTTGTGAACACCCCGCcGGAGTTCACGAAAATGGTTTAAGAAAGCAGTTTCGATTgATTGGA

Got

>CF810723 Length = 647 Score = 60.0 bits (30), Expect = 1e-08 Identities = 85/102 (83%), Gaps = 1/102 (0%) Strand = Plus / Minus Query: 4838 gctagtgagctcaccctatctttttcaggtacatggcaagaaacatgtgttg-gatgctt 4896 

           |||||||||||||||||||||||  ||||||||  ||| | | |||| | |  |||||||

Sbjct: 112 gctagtgagctcaccctatctttcccaggtacacagcatggagcatgagatcagatgctt 53

Query: 4897 ggagtgtggatgcattgcatgaagcttgtttgatcatgaagt 4938 

           |||||||| ||||||  ||||||| || | ||||||||||||

Sbjct: 52 ggagtgtgaatgcatgccatgaagattttatgatcatgaagt 11


>CV190427 Length = 537 Score = 58.0 bits (29), Expect = 5e-08 Identities = 35/37 (94%) Strand = Plus / Plus Query: 1616 aaaccaaaccgagccttatgtcccaataaattggggt 1652 

           ||||||||| ||||||||||||||||| |||||||||

Sbjct: 45 aaaccaaacagagccttatgtcccaatcaattggggt 81



5) DFR

I submitted the cDNA from grape ATTTTCTTCCCCGGATCATTTTTGAGAAGTATGGGTTCACAAAGTGAAACCGTGT GCGTCACCGGTGCCTCCGGTTTCATCGGTTCATGGCTGGTCATGAGGCTCCTGGAGCGCGGCTATACTGT TCGGGCCACCGTTCGCGATCCAACTAACGTGAAAAAAGTGAAGCATTTGCTGGACTTGCCCAAAGCGGAG ACGCATCTGACTCTCTGGAAGGCAGATCTTGCTGATGAAGGAAGTTTCGATGAAGCTATTAAAGGCTGCA CCGGCGTCTTCCATGTTGCCACACCCATGGATTTTGAATCGAAGGATCCTGAGAATGAAGTGATAAAGCC AACTATTGAAGGGATGTTGGGCATAATGAAATCGTGTGCTGCCGCAAAGACTGTCAGAAGGCTTGTATTC ACATCCTCCGCAGGAACTGTGAACATTCAAGAACACCAACTGCCAGTGTACGATGAAAGCTGCTGGAGTG ATATGGAATTTTGCCGGGCTAAAAAGATGACTGCATGGATGTACTTTGTCTCCAAGACACTGGCTGAGCA AGCTGCATGGAAGTATGCCAAGGAAAATAACATTGACTTCATCACTATCATACCGACTCTTGTGGTTGGC CCCTTCATAATGTCATCAATGCCTCCAAGCCTCATAACTGCTCTTTCCCCGATCACTGGAAACGAAGCTC ATTATTCAATTATACGGCAGGGCCAATTTGTTCACCTGGATGACCTCTGCAATGCTCATATTTACTTGTT TGAGAATCCTAAGGCAGAGGGACGTTACATTTGCTCCTCCCACGATTGTATCATTCTCGATCTTGCAAAA ATGCTTAGAGAAAAATACCCCGAGTATAATATCCCCACAGAGTTCAAAGGTGTTGATGAGAACTTGAAGA GTGTCTGTTTCTCCTCCAAGAAGCTGACAGATTTGGGGTTTGAGTTTAAATACAGCTTAGAGGACATGTT TACTGGAGCTGTGGACACATGCCGGGCCAAGGGATTGCTTCCCCCTTCACATGAGAAACCTGTAGATGGC AAGACCTAGAAGAAGAATTTCTGTCAGGCTTTCTAGCGAGAGCGTAGAAATGTCCTTCATTCTGTGGAAT CAAGGTTTTCTGGTAGTTTGATTTGGCCAACTATGTATGAAGTTTGTCTACTATTTTATCCATTAAAGGA AATTATTCTAATGGTCAATGTGCCGGAAATCAGAGTTCGTTC

>DW043076 Length = 475 

Score = 79.8 bits (40), Expect = 3e-15
Identities = 187/236 (79%)
Strand = Plus / Minus


Query: 562 gccaaggaaaataacattgacttcatcactatcataccgactcttgtggttggccccttc 621 

          ||||| ||||| |||||||| ||||||| ||| ||||| ||  | ||||| |||||||||

Sbjct: 407 gccaaagaaaacaacattgatttcatcagtattataccaacattagtggtaggccccttc 348


Query: 622 ataatgtcatcaatgcctccaagcctcataactgctctttccccgatcactggaaacgaa 681 

          || ||| |  || | || |||||||| || ||||| || ||||| ||||| ||||| |||

Sbjct: 347 attatgcctacattcccaccaagcctgatcactgcactctcccctatcacaggaaatgaa 288


Query: 682 gctcattattcaattatacggcagggccaatttgttcacctggatgacctctgcaatgct 741 

           ||||||| ||||| ||   ||| ||||||||||| ||||| || || || ||  |  ||

Sbjct: 287 cctcattactcaataatcaagcaaggccaatttgtgcacctagacgatctttgtgaatct 228


Query: 742 catatttacttgtttgagaatcctaaggcagagggacgttacatttgctcctccca 797 

          ||||| ||||| |||||| ||||| | ||||||||  | ||||||||||| |||||

Sbjct: 227 catatatacttatttgagcatcctgaagcagaggggagatacatttgctcatccca 172


>CV091566 Length = 829 

Score = 46.1 bits (23), Expect = 5e-05
Identities = 86/107 (80%)
Strand = Plus / Minus


Query: 715 gttcacctggatgacctctgcaatgctcatatttacttgtttgagaatcctaaggcagag 774 

          |||||| |||||||  | |||||||||||||| ||| | ||||||||||| || || ||

Sbjct: 509 gttcacgtggatgatgtatgcaatgctcatatatacctttttgagaatccgaaagctgaa 450


Query: 775 ggacgttacatttgctcctcccacgattgtatcattctcgatcttgc 821 

          ||| | || |||||| | || ||| ||| || |||||| ||||||||

Sbjct: 449 ggaagatatatttgcgcgtctcaccattttaccattctggatcttgc 403


6) F3’ H submitted from scaffold (near SSR primer)

ATCAGCCAAAGTCACCAGTCACAGACTATCAGCATGCACCCTGGGCCAAGATCAAACTGTCCGTGTGCAATTAAGGTTTGATTATAAAATCCCAACAGTTAAAAACTAAGTAGTCATTACATCACTTtGGCTACTTATATATTCACCTAGATAGAATCAACCAACAGGTTcGTATGGCGATTCAGAGACACATGAAATACCATTGATGACAGGATATAAACACACCAACACAAATGCCTATtAATACAAATGAAGAGCAAGCATGAATTTTGTGCACATCATAAGTTCATTAGCTAGGgCATAAATGGATACAATCTAAACGAGTCCTCAAAGTGCCGGCATTTtGTCTTGTTTTTGTCATTATTGTAAGAGCTGCTTCCTGCTgATAGTGCATTcTCTCCAAACTAAACCTACTAGGTACAAAACCTTCATGGCTTCTTCAAACCTGGGTGGCTACCAAAGTACTACTCACCATTACACTAACTGTACTGACAACTTTCTTTATCTTGTTAAACCTGCTGAATACTTATCATTTGTAGCTTGTTGCTTATGTTAAAGTTGAGCTTGTTAACAAGCGGCAAGGCTTGTACCATGATTGGGTTACTATGAGACTAAGAGCACATACACGTCTACCGGCCTCCATGTGACACAAAGAGCAGAGTGACAATGCAAGGAGGTAGTGCATAAATGAAGCTTACATTGGAACTTTGTAAAGTTTTAAACTTCAAAAGATGTGTTTTCTAAGAGTTATGTCTAACTTTTAGTTTTGAATGAAAAAACATGACTTTGCGAAAAGATGTTTAGAATCCTATAAATATAGGATCACCCCACTTACTTAGCCTAAATGACTTTTAATTCTAACAACTCTCCGTATTATTTTCATATATTATGGGcAaTTcGATTCCTTCTTGGCACCCCCGTCTtCCACTGATCGTGTAACCTGGAGACAAACTGCGAGATCTATGGAAGGTATCTTGGAGTCGTATTCAGCCTTTGGAACCTTCATGAAGGGAGGCAAATATGGCTTTAGGATTTATCCAAAAATATGGCTTTAGGACAGcGATTTGTCGTGCCTTTCATCGATTtCACATGCTAGTTTCCTAATTCTATTCATATTACATATTTGTGTGAttGTGTTCATATTTTTAGTGTTCAATGGCATTTCTAATTTCCTAGTGTTTTGTGATAATCATGTGACAAAaTCCCAACAGCTTATTCCATTCTAAGGCAGTAGTGAAGGAAACGACAAGTCAAAAAaTATGGACACAACCCGACACTCTACGGACAaCTGTCAAATACACCTAAATATGTGTCTAATTTTTCTGTACTAAATTTTCAAATGAACATGTTGTGGACTCTATAGGAAAGGTtGGGGATTCTCTGGGGCACGTTCAAGACACATAAGGAGTTAAAATGCAACTTTTAAAAGTGGAGTCAAAACTTCCATTGCTGTTGAGTACATTAATATTAATGTAGTAACCATTAATTGGcATAATGTTAATACTATTGGTGTTTTTTGTGTTAATTtGATAGAAATAATCTTTTAACTTTTATACGAATTCCTACGAATCATTGACTGATATAAATATACATTATATATtCCACGTGCCcTAACGTGCCCgAaTCTTAGTTTTTACGGAGAAGACATCCGTGTCCATTTTGTGTCCATATCCTTGCTTCTTAACTGTTAGATAATGACGATcAAAAATTCGATCACTCCAAcGATTGAGACACTGCGTATAACACCAAACTCTCCACCAACTCTCTCACAAGTTGATGTGTCCCACACcTGCGGGGCTTGTGCGAGGGAAATTGAGCTGTTACAGTCTAACTAACGAATGCCAATTCAGTGGGCTAATATTTAAGTTATTGTGTTCGATTTCTATGTTGTACTTCCATAAaGAAAAATTGGTGCATCTGTTGGAGTTTGTCCCACATTGGTTAATTAAAACCTCCAAATCTAGTATATGAaTCTGGGCAGCCTCTCCACTCATTGCCAATTGGTTTTGAGTTGGATGCATTAACATGGTATCAGAGCTAAGCTTTCgCAGGCTTTGGACCAATCTCTTGATTGATTGCCcTCTTgTtaGTGCGATAAAGTGCACCTTGTTTCAATATAATCCAATGTTACGGATCTTTGTTTACCTCTCCCCATGCGGGAaCTAGACAGGGGTCGCACATGCGGAGAGAGCATCTAGATCTCAAGGCAAGTTGAAGTGAGaaaaaaaaaaagaaCAGTTTGTGATAAGCAGCAGAACTGAGACATCAGATGCTTGCTTAAAAAACACCTTTCGATGTAATAAACAGATGGGCTGCCAAGCATGTGAccTTTTAAAACAGCaGTTAATAACATAGAAATCGAAGGTCTCCTATCTCACTACAATATCTATCCCCTAGACAATCCcCCTAAGTgTCTCTTtCGAGAcaGTTTTCACATTATTATTGTTATTGTtCCACATTGCTTGGGTATGGAATGGGAGATCACTTAATAACATATGGAACCTCTCCACTAATTGCCAATTGgTTTTGAGTTGGATATAAAGTTTCCAAATGGTATTAGAGTGGgCCAATTCCATCCCAAATTTCTTAACAAAAaTTAAATCAAGCCCACACCCCACGATGACAGACCCAGGAAAGGTTGTACgATGATAGAACCCAAAAATTGGCCACAGGTGACAGACCTAAATGTGGCTGCAGGTGAGGGGGGAGTAAGAATAGTTGTCTCATATTGCTTGGGTATGGAATGGGAGATCACTTAATAACATTTGgAaTCTCTCCACTCATAGCCAATTAGTTTTTGAGTTGGATATCCACAATTATTTCAACATTGACTCAACTGAGCACCTTTATCAATCTTTCATATCGACTCCCGTGTACAAGAGGCAAATCAGTGCAAAATCACCATAATACATAAAGCCAAAGCTTTCAAATATTCAACCACACCTGATATAGTCTCAAAGCATTTGAATTTCCAATTAGAGCTTGCAGATTAGACTTGGGAAGATCCAGCAGTTGTTGCACATTTGAGAAACCCCTTCTGCTTAGGGAACCGAAGAGATCATCAGTCATGCATGGCAACATCCATAGAGCAGTGTCACTGCCAAACCATAAACCCTGCAAATGAATCCAAAAATCCATCAAAGTGTTGCATGAAGAGGAGAAAAATAGCAGAACCCACAAAACTAAACCTTCAAATGGAGGCTGTTTAAATCTTCTTGATGGACAGAATAAAAAGAAAGACAAGTGTAAGAACCAGGACACAGCTCCAGGTTATTTCCAGGAAACTATTACTGCAATGGATAACATGGAACaatttATGTTCGAGAAAGAAAACCAGGTCACCTGTCCTGCAAATAAcTTTAGAAGTAGCCAATTTCCAAGGGACCCCGTGaGAAACCCTCGGCCGTCCGATGGTGTTTTGGACGGCCCGGATGTGTTAAaCACATTTGGGCCaTCCAAAACACGATtGGACGACCGTCGATAAGAGTGGTACATAAAGAATCGTTTATTCCCACAAGAAGATGCCTATTCAAATGGTCGAATACAGTTCCCTAAAAGAACTTACAACTTACAAACTTTGATAATCATCCCTTAGCTGTGGAAAATAGGTCCGATTCACTTTCTTACCGAAGGGCGGGgAGGATAGATTGATTCAtCTAGCCTGTCAAGTAGAGCCCCCTATAGACGTCAAGACTTGACGGCCTAAATGGGTGTATCATGCCTTGGTtATTGATCCCAAGGCCCAATCCAGGATGAAAACTCCATCTATAGATCTCTAATGAGgACACAACCTTAACAcGATGGCCAAAAaTAGAAGCTTGATGCGTGAAACTTCTGAAGAAAAAATTAACCCAAAAAGTTGATGTGTTTCGAGAAACATAAAGAAGCAATGGCTAAAATGACAAGATGATATACTGGTAGGTACAATAAACTACAGtGGGTAAATACAACCAACGAAGAGAGCCTCCAAGTTTAGATCTCCCTATTGAATATTGGAGACCAATTCGTTGCTGCCAAGGTCAAAATTTGAAAGAGGCATAAACTAACCTAAGAATAGAACAAAACTTTTTATTTTGACTTCTGGAGCTTTGGAGGTCTAAGATAATCCCAAAGTCCAGCGTGGTTTCTCTGGTCACAGAgaGAAATTTTTCCTAAACgaTACAAGAGATAAAGATGAcAACTATTAACTACCAACAGGGATAAAACCATGGTAAAAATACAAACTATACATAGATTTTTGGGTTATGGTTTACCCACcATTTTAATAAGTGCAAGTTCAGGAAAAGGTATACATGTATGTACCTACCCATTAATTCAAATTGCTGCACTGATGCGATTAACACAGAAACAGAATGTACTAGAAATCTTTCACAAGGGAAAAAAaTTTGCTTGGCCAGAATCGACAAAGGCAAGTCTAGTAGTACCAGCAATAACTTGGTCAATACAGAAAAATGCAACTTTTGCATCTTTTCTGAAATAATTGTACTATATTCCGCTTGTTTAGGTCTGTTTCGCTATTGGgTTGTCTTTTTGTGTTCAATGGAAACATAAAGCTTCATAATtCCACCCGTGGTTCCTTTCGGTCTCTTAGGAAATGGAAAGAGTTCAGCCATTTCTACAGAAACAAATATGTATTAATTTCATACTTCAACGAATGTCGCTTTAACgACATACCACAAATTCACCGATGACTTAATTATTCTGGATTCACAATGACTGACTTAGCATCATATCGAGAAGTGCCTAGCAGTATCTGTGAAAGACCAAACATGTATTCcTGTCCTCATAGATTgttGGTACAAAACTTGAATTGATCGGGAAAGAAGTGCCTGGTtGGAAATCATAGCAAACAAACTATAGTAACAGCCTCAAGTGGTGAAGGCATTCCCAATGTAGATCCTGAAGAaCTTATATCAGACAAATGTTGCATTAACAGTCAAACTACAAAGTTGAACATTTTGTACTTAGTTGCATGATGGACAATATTTTACCTATCATTCGCAATGAAACTGGCAAAATCACTACATGGGCGAAATAAATAATCAACTCTTCTAACTAGTTCAATgTAATTTCAGACATCCAGCGTCCACTGTCCACTCCCAATTTTCTATAGaTATGAATAGGATTATTTCATACGACATGATAAAAGAGGTCATCATAAAAACGACGAAAAACTCCTCATAAGAACATTCGGcAGAATGCAATATATTGTTTAAAAGCAAGCTTTTAACAACAGCAACCATGTGATTGATGAGACTGATGATTAAGATGGAAAAGACAGTACATACAGAATTAGAATCACCATTCTATTCTTATCTATCAGACCGTTCATCTACTGGGAAAAAGCAAAAAAGCAACGACCGGGAGAAATACCTGCATGACCATTTGAAGAAGATGCATACAGGTGATTGTACTTGAAAGCCATCCACTGTTCGCACATATATCTATCATTGCCTGTATGATACGTATACTTTGATCGAGCACAGATTTCAAGTCTGTGACATAGTCTGTGATtGGTAATTCTAACTGAGAAAAGTGGGCCTGCAGACCAAACATCATATATTTAAGAAGATAAGACCCCAAGAACAAACACAGAACCATGTATTATATCCAGAAGACAGTCAAAAAGTGATGTCGACCTGGAAAAGTAGATTCGCTTTAACATGTGGATCATCTAAACGATTCTTATCCACCATATACGGAACTTTCACAGACAATGCTTCATTGTAGTTTTCCTAGAACCAAAGAGAGGATGATTAGAGAAAAAGTCAACAGACATTTCATGTGTTATTAATATAAACTTTGAAGATGAAGCCACGACAAACAGCCAATAAATCATATAGCCAATAAAAGAGTGCATCATCTATGTGTCCAAGAATtGGATTCATCGACTTCTCAAGCCCAAAGACTAGGGAACATCAAACATTCAGATTTTTTTAATATCATTCTTTCATGTGCTCTGGAAAAACTCTCTTCACAAAaCATAGATCATTACGCATAGTTGTTCAGCtcTTTtTTTTTTTtAAAaGCAACAATGTCAAGgAaGTAGCACTGTTCCTGAGTTAAAATATACACCAACACGTTAAACTGAGACAGCGAGAAACCCTtCCTGGTTGGTGAAAGCAAAATCAGAATTTATTAGTTTGATAATTAGCAAAAAAAAAaGATGATCACAATTAGAAAACCACCTCATTATGCCTCACAGGAAGCTCATCATATTCGGAAGAACCAGACAGTATGTGAAGGAAAACCTGCAACGAAAAACAAATGAAGTGAAACATTGCTTAAAAAGGGATGAAAATGGAAGTTATACCGGCTATATGTTGGAGTTTTTACTTCAAGAGATGTATCCGGGCCTATATTTGAACCAAACATTGAAACAGTCATGTACTTCAGGTAATACTGAGAAGCTATTGAGCCCAACATCATAGGTTCCACGCTGTCTTCATTCATCTTCAAACACCCACTGTCTTCCAGATCCTCAAAGGTGTTTTGCACCAAGCTGCCAAACAAAACGGTTATAGAAAATTGCACTACAACAAAACCTTGGGTCATACAGTAGGTTATTACCTTGACAAATATGAACTCACAATTTCAGGTCCAGTATCCtCTAAGCCATAGTAAGCTGGATTAACCATCTGTCACAGGGATATATTTTTTGTAAGAAACTTTAGCAGGGAAACAAAGACAGCTGAAAGCCCAaTGCTGGGTATAGAAGCTGTGGTGTGATTCCATCCTCAGTCGATCATTGTTCCAAAGATGAAAATATCATAAACATGCAcAATTGAACAcTATAAATGCATCTAGTCCATTATTTCAGGGAACATGACatGCATGTTTTGGGCAATCACTACAATCATTACGCTGAAAATGCAAATTGAGAAtAGCAGGTATCCAGGTCAACTTCGTGCTTATTATGAGGCATACATGGCATCATTATTTTGCTAATAATGTTAaGCAAGAGAGTGTCTCTACGCATTGCTTAAGTTGAGACACCAAATCGAGCCTTCCACATCACcGAACTCGATAATCATTATAGACTAACAGAGTTTTAGTAATTCACTCCATACGCTCTGCTTGTTATCATCTACATAATTATATTAATGGGTTTGATGCCTTAAATAAATCTTTCCAAACAGCCGGGAAATAACCAATATTTTACTAGATTTTAATCTCAACTTCCAAGGGTACAAAACATTTAAAAAATATTTGGTATTGTACATGACCAAATAAGTACAAAACAGTTTCAAAAACTTTTCTGTTGTTTTCTCAAAATTGGGTTGTCTTTAGAGAGAAATATTGTTTGCCAAACAAGATTGGTGTTTAATGGAAACGGAAAATAGAAAAGCAAAACAAaCaaaacaGGTGATTAGCTACCATAAGTTACAACTACTGTTCAACTTCAAAGAATTTAGCATTTCTGTTTAAATTAAAAGAATCTAATGTTAATCATGTGAAGAAACCAAAGAAGCaCTTCCAACAGCTAAACTAACACGGAAAGTTAACAACCTGGTCATATGCATTTTACATTTTCCCCATTTTACAATGTGCTCCCACAAAAATGTGAAAACGTGTTCTTATGTAACCTCTCTTCAAGTTTCGGATACAGGACTCTGCTTGTTGACATCAACCTTGCATAGATGTTCAAAATATTTTCTGCCATTATAAGACAAAAGAAGACCATTTTTTCTCTTCCCAAACTTTTGTATGCATCTTCCCAATATTTAAAATGTGCCTCACATCCCAAGTGTAAGTGTGTGTGTGTTTAACACTGGATGTtCTTTCCTGATAGGTGTAAGTGAATGTAATCAACATAAATATAGTTTGACAaCCGAGAGAATGTCCAATATATCTATAGAACACAAAACAAGAACTCCAGTCTCtAGTACACGCTTTATCCTTCTGAAATGAGTATAATAAGAAAATGAGGGgAAGCCTAGAATAAGTCAGGACCAAGCAAGGTGCTTTAAGCgCTGATTTACTTATTGCGCATAAAAAGGAATTGCcGAAATTTTACCAATCTCCGAAACAAATAAGTCCAGGTGAGATAATGAATTGCATCCTCTCTATGGCAAATTGTTCCAGAAACTATCTCAGCATTAATGTGGTCATGCAACTGCTCCCTCAGACTACTTTCAACTGGGAATGGTTCATACAGAAACTGCAACAAATAAAAC


>CV190608 Length = 474 but this is for RPC-1 which is an RNA polymerase 3 so I don’t think this is right 

Score =  139 bits (70), Expect = 3e-32
Identities = 111/125 (88%)
Strand = Plus / Plus

Query: 1924 gagtttgtcccacattggttaattaaaacctccaaatctagtatatgaatctgggcagcc 1983 

           |||||||| |||||||||||||||| ||||| |||| ||||||||||| ||||||| | |

Sbjct: 137 gagtttgttccacattggttaattataaccttcaaagctagtatatgagtctgggcggnc 196


Query: 1984 tctccactcattgccaattggttttgagttggatgcattaacatggtatcagagctaagc 2043 

           |||||||||||| |||||||||||||||||| |||  ||| |||||||| ||||||| ||

Sbjct: 197 tctccactcattaccaattggttttgagttgaatgttttaccatggtattagagctaggc 256


Query: 2044 tttcg 2048 

           |||||

Sbjct: 257 tttcg 261


7) FLS submitted from scaffold near overlap sequence with query GAGAGAGAGGgaGgTTGcTGCAATCACGCTCTAGTATCGGTtGTGAACTCTGCCGCAGGAGAGGTGCGAATGGTAGGTGACGTCagCGGCGATGCGaTGCTGGACGCcGGTGATGGGACTGGGAGGGGCTTGGGGTTTTGAAGGACCAAAGGGAGTAGACCATTAGGAGAGTtAGGTGGAGGTCCAAGAGTGTCGTCGTTTGGGTCTGGTGGGTTGTTTCGCCATGGAGAGGTGGTGGTGGTTGGCAGTGAGAATGGGAGAGCTCAGGCGACATTATTTTTGAAGGATGTTTtAGAGGTGGTGGTTATtAATATgTGGgTTATGTAGAGGAGTGGGgACGGATACGACACAACACTCTCCGGACACGTGTTGCTTAAATACTTGTCtAATTTTTtCTTGTATTTCTTTTATTCAAACACATGGTAGATGTTCAAGGACACGTTGGCAAAACTCATAGAGCGTGCTAAGGACACATAAAAGGTTAAAATaTAATTATTAAAACTtGGCCATAGGTGAAAATTtCCACCGATGTATAGTCATGTATACTTAtgCgCAGACTGCTAATTTATCTTTGTTGtaTTaTTTCTAAATTAGATAGAAATAAAGATTTCTAATTTATATGAATTAATCACATACCTCTATGATACAAaTAAaTTTtATATTGGACGTATCCCCAACGTGTCCTTGTCCTAATTTTtGAAGAAATAACGTGTGTGCGTATCCGCATCGTGtcGTGTTCGTATCCCgTATCCGTGtCcGTGCTTCTTAGACAATAAATATTGATCgTCGGTAGCCACACAGTTTACTAAGGGGCAAAATGTCTAACTAGTTGGATTCAaCTATAGCAGTAGCCGCTAAAACTCTGTAGGCTCgCTCTCTACTCCTATGGTAGCCTATGCTGCTAACTCAGTTGAtGGaTAGCATGGATCCcATAGAGCTCTCTCAGTCTCTAGCCTTGTTTATGGCTCAAATCCCTCCTCATCTCCAGTTTCACATCACCGGACAGCTATATAGCCAAGCCTATTGTATTTTTtGTATGAACACTATGGAATCTTTCATTCCgAaTTCAACACGGCTATCCAGACGGAACCTGAAGTGAGGCTTTAATCTGGGTTCTCCCATACGTACCCTTTTtATTGAGAATGCAAGGCATTTACGGATTGACATCCAAAATAAGATaCAGACATGGGACAAAGCTATGTGAATGCCGTGTtAATTAATTGGGGAGCTATTCCATTAGTATTAATTTTGTATACCACAGAAACTTCTTGCCTCTAGTtGTCCACTTTTCCCTctcATCCTCTCATTCTGGGTGACCCTTATATGTGCATATATATAGATATCAACCCACAAACGCAAGGTTGATTAGTGATCATAGAAaGAAAAAaTGATGGACCAGAAGGAGAGAGTGCAGGCCATTGCCTCGGTGTCAACAGACACCATACCGGCAGAGTTCATCAGGCCAGAAAGTGAGCAGCCCGGACTCACAaCCTaTGGTGGGCAGGCCcTTCAAGTCCcGGTCATCGATTTCTCAGACCCGCACGAGGAAATCCTCGTGGGTTCCATTGTCGATGCAAGTTGCcAaTGGGGAATGTTCCAAATTGTGAACCATGAGATTCCAGTAGAAGTAATAAGAAAATTGCAGCAAGTTGGgAAAGAGTTTTTTGAGCTCCCACAAGAAGAGAAGGAGGCTTATGCTAAGCCTGTTGGGTCAAAGAGTGTGGAAGGGTATGGAACAAAGCTGCAAAAGGAAGTGGAAGGGAAGAAAGGTTGGGTTGATCATTTGTTCCATAAGATATGGCCTCcTCATACTATTAACTACCAATTTtGGCCTAAAAaCCcACcTTCTTACAGGTTCgTGATaCATTTtCTTCTCTCTCGATTATTCgTTAGGGgCAGATAGAAGGgCAAACAaTTAGAGTACTACTGTTTTGgCAAAAAaaTGGATtATTTTTTTGTCTTTATTCAAaTAAAATTTGCATTtgTtAGTTTTGCGTTGATTTTTTGTGGATTATTagTTCATCTCGACAAGATGAATCTAAAAAGTCAAGAGTTACTATCAAAattaaaaaaaaaaaGTTCaCTAAAGACAATAATtCAAAATTTtCTCAAAaCACACATTCATGCGATTCGACATTtGTCATTACGCATGCGTATaTTGGGAaCATcTGATCTGCaTTTtttATTttGgTtATTTTtCTTGGTGTcTTATAAAAATGTTGtGTCTtGTt

>CV091044 Length = 735 

Score =  363 bits (183), Expect = e-100
Identities = 306/342 (89%), Gaps = 5/342 (1%)
Strand = Plus / Minus


Query: 371 tgcttaaatacttgtctaattt-tttcttgtatttcttttattcaaacacatggtagatg 429 

          |||||||||||||||||||||| |||||||||||||||||||||||||| ||||||||||

Sbjct: 571 tgcttaaatacttgtctaatttgtttcttgtatttcttttattcaaacagatggtagatg 512


Query: 430 ttcaaggacacgttggcaaaactcatagagcgtgctaaggacacataaaaggttaaaata 489 

           || |||||||||||||||||||| |||| ||||| |||||||||||||| ||||||||

Sbjct: 511 ctctaggacacgttggcaaaactcttagaacgtgccaaggacacataaaaagttaaaatc 452


Query: 490 taattattaaaacttggccataggtgaaaatttccaccgatgtatag-tcatgtatactt 548 

          ||||| |||||||||||||||||||||||||||||||| |||| ||| |||||||||  |

Sbjct: 451 taatttttaaaacttggccataggtgaaaatttccacctatgtgtagttcatgtataagt 392


Query: 549 atgcgcagactgctaatttatctttgttgtattatttctaaattagatagaaataaaga- 607 

          ||  ||| ||| |||||||||||| |||||||| ||  ||||||||||||||||||| |

Sbjct: 391 at--gcaaactcctaatttatcttcgttgtattgttcttaaattagatagaaataaatat 334


Query: 608 tttctaatttatatgaattaatcacatacctctatgatacaaataaattttatattggac 667 

          ||| | ||||||||||||| ||||||||| ||||| ||||||| |||||||||||| |||

Sbjct: 333 tttgtcatttatatgaattcatcacatacatctataatacaaacaaattttatattcgac 274


Query: 668 gtatccccaacgtgtccttgtcctaatttttgaagaaataac 709 

          ||| |||| |||||||| |||| |||||||||||||||||||

Sbjct: 273 gtaaccccgacgtgtccgtgtcttaatttttgaagaaataac 232


8) LDOX submitted from scaffold AAGCTTCGTTTAATTTTTCAATATATAAGAAATGCCAAAAAATGGTACATTGAGACAGTGGAGAGGGAGAGAAATCCAACCAAAAGTTAAATAAGAAAAAATTAAAAAACCACCCAAACATTAGACACCCGAGTACCCAACGGCCTCAACCTTATCGCCTTTATCGGTGAGGTAGAGACCGTGTCAATGCCACCGCTGCAACCCACCACCAACAATCGTTTTAAGTTGCATCCAATTATGTTTATTTCCATAAACTAGCATAAACATTTTTCAATTTTTAGTCTACCTTTTAGGTATGGAATTTTTCATGATGTTATCTTGAAGTTTGGCTCTACCGACTGACTAAATTTTCGTGGCTCCGCCCGCGCGCAGGTGCCAACCATTGATCTAGAAGACTTGGTATCGGAGGACAAAGAGGCCAGAGAGAGATGCCACGAGGCGTTGAAGAAGGCGGCGACAGAGTGGGGGGTGATGCACCTAGTGAACCACGGCGTCCCCGAAGAGCTGATGGACCGGGTTAGGGTGGCCGGAGAGGGGTTCTTCAATCAGCCCGTGGAGGAGAAGGAGAAGTATGCTAATGACCATGATACTGGGAATTCTGGGAAGATACAGGGGTATGGGAGTAAACTGGCAAACAATGCCAGTGGGCAGCTGGAGTGGGAGGACTATTTCTTCCACACTGTTTACCCTGAGGACAAGAGGGACATGAAGATTTGGCCCAAGAACCCATCCGACTACATGTAAGTTTTTTCTGAATAACGTCCATAGATTTGCTGTTTACATTAACGGGTCCGAACATGATGTATTTAGAACCATTCTACTCCGATAAACCTGGTGGTATAGAAAGGGGCGTACAAAAAATGTACACAGCGCGTTTGGGGACCCATTTTTGGGTTCCACATGAGTGATCCGAACCGTTCATTGTTCTAAAACATTTTTCTGAGGGGGATCAAACAAATCAGCTCAATCGGACGGTTAGAAGTGCTTGAACCAAAGTCCCACCGTTTCAAATAAAAACTTTTCATTTGAAACGGTGGGACTTTGGTTCAAGTACTTATTATAACCATCCGATTGAGCTGATTTTTTATGGGCCCCCTCGGAAAAATGTTTTAAGAACAATGAACGGTTCGGATCACTCGTGTAGGACCCAAAAGTGGGTCCCCAAATGCGCTGTTTACATTTTCTGTACACCTCTTTCTGTACCGCCAGCAGCGTCCGTTCTATTCAAGTAGGAACTACAAAAATTTATTCCACTAAGACCGCACATATCTCGAGTAATTTTGAACTTAATTTGCAGTACAATTTACTAATGTTTTATGCACCG


>CV190548 Length = 671 

Score = 65.9 bits (33), Expect = 5e-11
Identities = 40/41 (97%), Gaps = 1/41 (2%)
Strand = Plus / Plus


Query: 701 gggacatgaagatttggcccaag-aacccatccgactacat 740 

          ||||||||||||||||||||||| |||||||||||||||||

Sbjct: 3 gggacatgaagatttggcccaagaaacccatccgactacat 43


>CV191078 Length = 651 

Score = 65.9 bits (33), Expect = 5e-11
Identities = 43/45 (95%), Gaps = 1/45 (2%)
Strand = Plus / Plus


Query: 697 aagagggacatgaagatttggccca-agaacccatccgactacat 740 

          |||| |||||||||||||||||||| |||||||||||||||||||

Sbjct: 2 aagaaggacatgaagatttggcccacagaacccatccgactacat 46

9) MybA1 did tBLASTn using amino acid results from scaffold03500 LGVRKGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG


>DR067494 Length = 369 

Score = 43.5 bits (101), Expect = 2e-06
Identities = 18/35 (51%), Positives = 24/35 (68%)
Frame = +3

Query: 2 GVRKGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG 36 

          G+ +GAWT  ED +L + I  +GEGKW  +P RAG

Sbjct: 144 GLNRGAWTAMEDKILTEYIRVNGEGKWRNLPKRAG 248


>CV091396 Length = 791 

Score = 41.6 bits (96), Expect = 8e-06
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = +1

Query: 3 VRKGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG 36 

         ++KG W  EED +LK  +E HGEG W  V  ++G

Sbjct: 19 LKKGLWKPEEDRILKNYVETHGEGNWASVSQKSG 120


>CV191530 Length = 673 

Score = 39.3 bits (90), Expect = 4e-05
Identities = 16/32 (50%), Positives = 21/32 (65%)
Frame = +1

Query: 5 KGAWTEEEDCLLKKCIEKHGEGKWHQVPYRAG 36 

          KGAW+++ED  L   I +HGEG W  +P  AG

Sbjct: 148 KGAWSKQEDQKLIDYIRRHGEGCWRTIPQAAG 243


>CV090776 Length = 717 

Score = 38.1 bits (87), Expect = 9e-05
Identities = 17/34 (50%), Positives = 23/34 (67%), Gaps = 1/34 (2%)
Frame = +1

Query: 3 VRKGAWTEEEDCLLKKCIEKHGE-GKWHQVPYRA 35 

          V+KG W+ EED  LK+ I+KHG  G W  +P +A

Sbjct: 103 VKKGPWSPEEDAKLKEYIDKHGTGGNWIALPQKA 204


10) MYCA1 submitted from scaffold 00278 (BLASTn) tacattattttcctttctttctttCTCCTTTCTGACTTGATTGACaTAGTATTAACCGATACGATAGTATAATAGAaCAAGGGATCTACCCTGTTCCCTTCTCCTTTACGGTTCAGgAATTCATCTCCATTAGTCATGAACCCCGcTTGgCCcGCCATTGAGTCACAACTCTTCTtGTACAAACTTCTTTCAACTgaTCAGTCGATAGGAAAGTCGGATGACTCAGATGCCTCTACTGTAAGTaGTGGGAggtGCATAATtCATTATACGGGGGaCCAAATCACAATTTtgAGGGAACATTCCTTCATAATTtATACAAACAAAACCTAAAATATAGTACCAATAATGAATAATTGGGAGgTGTGCCTACCCGGGCCCTCGTATCCACCCTACCTGcAGGCTGAAATATTTCCTTTGAATATGTTGATGTAGTTCcCCTCAATTTTTTATTTTTATTTTTTAGAAATATCACCGTTTGGAAAATCTCAAATAAAgAGTAGTGGGATATAAAATAAGGAATCATTTCCTCTCCATACTCAATCCCTCCATgTCTTTCCTTACTCTTAATATTCATTCATATTtCAGGGCCAAAACAATGATCCTAGCcGTTCACATCGTAGAACACaTAGAAAAATATAGGTTTGCAAAATATCAATATGATCATCCATGATAAATTTGTGAATAAGTTACCTTTTGTTTCGTAGATTGTAAGGTTAAACTGTAGAATATATTTGCCATTCATTTTGCATAACAATTTGTAACATGTTTACATTGTCATTTAAATTTGACTTTATATTTGAAACTTTATATTATTTTAATTAGTTAATTAATTTGTCTTAACAATGAATAATAAAAAAAaaTTcTAAAaGTGAaTAATATTACAATCACGTTTAAAaGTGAAAAAaTaTTACAATCAACGTTGGCAATGTTCAGCCCTAATCAATATTGCCTCCAAATTGTGGTCCCGGCACAaCGCCCAGTACGTGGAACCACAAATGAAGGCGCGTGTCATAACTTAAAGCCCATCCTTTCACTTCATCCGACACATGCTCCCACCActATATTGGGATGTGTGGTACAaAGTGATGAGGGTGCAAAAAAAGTTGCACGAAATGATTGCTAATACGGGCAATAGCTATTTCTTTGCTCTGTTGGACTGGAACTGGAaGGgATCTCAaGGgCAAGTGAGTATAACAACCTCCAACATTCACGTCAAATTTTaCAGCACAAAGTTGTAACGaGATGCAATGACTACCgCcAAACTTATACAGTCCATCCAATTTGATTCTGGAGCTGTAGGTAGGAAATAACtcAGTGACAAGATATTAAaTCATTAAAAAaGAGAAACAAGATATTAGAGCATATTAAAAAGATAAAaGAGAAAaaaaTATtAATAAAAaaGaTAAATAAATAATtATATAAAAGAAaTGAATTAAATAAAaGAATTGAATTAAAtAATTAAATGAATtAGTAaTATAAaGAAATtAATTAATATAAATAATATAAATAAAGgAGGGAAGAGGGgTAAAaGGGTAAAAGGACAACATAAAGGTGGTGGGACAACATAAAGCTTAACTGAATCAAGAATTTGACTGTTCTATGTTCAaaaAAAAAAAaaaGGgAaTTTGACTGTTCTAGGTAAAAATAaTGTCTTTAAGAGCTCCCAAACAAGAGAACTTATTATGAAGCTGATAAATAGTTGAATTTGCAGTCTGCCATTGCAATTCCTCCTCAACCCACCaTAAAAGCAAGTGGGTCTTCTTCTTTTGAGCTTGAGAGCAGGATCTGATTGCAGAAATGGAAGATTAAGAAGAAACGCATCAAATCCCAGAGTGAGTTTCTTATGTGCTCTTTAACAAGATAGTCTTCTCTGAATAATCTACTTTCTTCCTATTTACTTCCCCCCCcAAAAAAAaTCTACTTTCTTCCCTCCAAATGACTTTTTCTTGGTTACAGCCTTTTCCCCTTTTCACTGTGGTTGATGGCTTTTTCTAACCTCAACATGACAACATCAGGTGCTTATGAAAACCCTGATCTTGATTTTTTATCTTATACAAAAGCTGAACTAAAGCTACTTCAGAAATCCTAAAGTTCTCCTTTAGAATGGCTTTTCTAGTTTTTGCTTTGCATCTTTTGACTAACTGAGTAATGGGTTTTCTTAAAATTTGGTGACtaGAGTTTTGAGGGTTATGTGTTGGGgAATGGCTAAAGGGGCACAAAACCAACAAGGGGTGCCAGAAAATCTGaGAAGGCAGCTTGCTGTTGCTGTTAGGAGTGTGCAGTGGAGCTATGGAATTTTCTGGTCATTGTCAACCAAACAAGAAGGGTATGGTTTACCTTTTTACTATTTTATTTATCTTCATATTTTTATGGTATGTCCTTAAACATTCTATTTCTTCAGTTTCTGTTGCTGGTTTTGCATAAATTTCCAGATCTGGGAAGAATAACGTCTAGTTATGCTCATTATAAATGCTTCCCATTCCATAAAAAAaTTGATTTCCCTGCTCATTTATGGCCTCTTTCCTGCAAATGTTAATACGCCTGCTGATTTATGGCCTCTTTCCCTGCCCAATATTTATGTTCATgTTTGCCTAGTATGTCATTACATAATTACATTTTCTATTTCTTCAGTTCATGTTGCTGTTTTCTTCATTATTTTTTGATAAAATAAAATAAAACAAAATTTCGTTGagAAAGCGACGAACGAGACCAAAGCTTCCCTTACAAAGGGTGAAATGCTGAAAGGGAAgAGATTGAAAGAAAAGTAGCTTCAAAGTTCAAACCTAACACAGGATAATGCTTTtCTAGGGAAATCAACAATTTTCATGGGAAAGCCATTGCTTATAGCAAAGTACACCTACATGCCTTGGTACATTGAAATTCCTCATGCTTAACTGATAAAaTATGTTCCTTGTTATTTTGTTAAGGGTATTGGAGTGGGGTGATGGGTACTACAATGGGGACATAAAGACGAGGAAGACAGTGCCGGCCATGGAACTTAAACCAGATAAAATGGGTTTGCAGAGGAGTGAGCAACTGAGACAGCTTTACGAGTGTCTCTTAGAGGGTGAAGAAAGTGATCAACAAGTGCCTTCTGCCGCACTGTCTCCAGATGATCTCACAGATGCAGAGTGGTATTACTTGGTTTGCATGTCCTTTGTGTTCAACCCCGGCCAAGGGTACAAGCTTTCTTCTGGACATTTTCATCTTTCTCTGTGgCTGTGAAAACTTaGTTACACTGAAAACTAAACAGAAAAAACCGAGATGTGACCGTTCAAATCTAAACAGAAAACTTAAAGTTTTAGAGTTTCTGAACTCTGCAGAAATTCCTGTGTCCATTTTTGTTtATGAACAAGAAGTATAGTAACTTGCAAATATtATGGAGTAACCGATATTGGATCATTTGTGTGTGTTCATTAAGATAATGATCAGTTTTTGAGGAAATTGCTGTGTAGTTTGCCAGgAaGGGCATTGGCAAATGGTCAAGCTATCTGGTTATGCAATGCTCACAGTGCTGATAGCAAAGTGTTTtCTCGctCTCTTCTAGCAAAGGTCcGTTCTTCTGGGGCTGTGCTCCGTTTTAGATTTCGTGCCAATTTTAGCTTGTTTACTAGACAAGTTTtACGATATtCAGCTCAATATACTCTCTACTAGTTAACTAACAGTGATGCTATAATTTTCTTCCTTTCTCAAATGGTTGGACTTGCGCACGAATCAACCTCAGAGTGCATCTATTCAGGTAAGCCGTTATTTTTATTAATTGGTACGCAAGTTTGCAAGCACACACATTCACAGGCACACGCACATAAGGACGTTGGTGGCCCCATATAATAGATCAAACAAGCAGTGAAGAATTTGTTTCCGTGGAGAACGTTTTTTTAGTAATTTTACTTGCAAAAGCTTTTCATAAACGAATCTTGTTGCATATACCGGTAGTCTACTACAATGACCATTGTTTtATTGCAGACTGTAATTTGTTTTCCCTATTTGGGTGGAGTCATTGAGCTAGGCGTGACGGAGCTGGTAATTTCTGTTCTATTGCTGGATTTACCACTATTTTTGTATTGAAGATGCATCAGATGCTATACGAATTATCCCAGAAGTAACTGGTTCTGCTATTCTTTTGTTTTCcTAAGGTATTGGAAGACCCTAGTCTCATTCAGCACATCAAGACTTCTCTATTAGAGTTTTCAAAGCCCGTTTGCTCTGAGAAATCTTCTTCTGGTCCTCCCCACAGAGACGATGACGCTGACTTCATGTATGCCAAGGTTGATCGTGAGCTGGTAAATACAATTTCGTTGGAGAGTTTCTATTCCCCAACTGAAGATATGAGATTTGATCGGGAGGGACTAAATGACTTTGAAAAAAATATCCAGGCCGATTTCACTATGGGTTCTCCTGACGAATGTTCGAATGGGTGTGAACACAATCACCAGACAGAAGACTCCTTCCTCCTTGATAATGTAACTTCTCAAATGGAAGATGATTTTAGCAACTGCGTTCAAGTTTCCATGAATTCTAGTGATTGCATATCTCAGGTCgTTGTGAACCAAGAAAAGTTTATTTCTCCTCCCAAGGGTGACAATAGTAAATTGAAAGAACTTCAAGAAGGGAATGATACAAAATTTGGATCCTTGGACATTGGAACTGATGATGACTTGCACTACAAAAGAACTCTATCTGCTGTTCTGATAAGAAATTCTCATAGGTTACTTGATAACCTTTGCTTCCATAGGTGGGATTatAAGTCTAGCTTTGTGAGTTGGAAGAGAGGAGAAATCAGAAATGGCCATAGACCACAGGTGCCGCAGCAGATGTTAAAGAAGATATTAGTTGATGTCCCTcTAATGCATtGTTTCTCACTCAAGTCTCAACAAGGAAAtgTGGGGAAATTTtGGTTGTTAAAACAAGAAAGTTATGTGCAACATGATTTGCCGGATGATGGAATAGAGAATGAGAAGTTTTtGGtCCTCAAATCGATGGTTCCTCCTTCTGTTAGCAAGGTAATTCAACTGCCATCTATTGGAAAATATGATCGAATGACTCAGAATTAAATATTTCTTTACACTTCTTGCTCTCAAGACTTGCTCATTTCTATGTAGAAAATCAGGATGGCAGAAGGCATTGACTTAATCCCCACCCTCAAAACAGAAGGATTCGAATTTTCTGAAAAACAACTTTTCGATGTtGCTAATTTTAGTCACTAAAAGTAGATTTTCAAGAATAACATAATTTTCTTGGGGTGATGGTATACTATATTTGaGTCCTCAGCATGTCACATTACCTTCACTAATCAGAGGGAACATATAAACAACATGGAAAAACGCACAGCAAAAAAGTAGGAAACGGGAAAATCCTAATCTTATTCATCATGCTTTGCAGGTTGACAAAGCAGCAATACTTGGTGACACTATAGAATACTTGAAAGAACTTGAGGGAAGGGTAGGAGAACTTGAATCTTGCATAGATTTAGCGGAGCATGAAGCTAGAAGAAAcTACCCAGATGAAGTAGAGCAGAAATCTGAAAACAATGAAAACAGAAGGATTGGAAATAGCAATAAGCCTTGGATAAACAAGAGGAAGGCCTGTGATATTGATGAAACAGACCCTGAGCTCAGCAGGGTTGTTCCTAAAGATGGGCCACTGTTTGATATGAAAGTAAaGATAAAAGAGCAGGAAGTCCTGATTGAGATGAGATGCCCTTCcAGGGAaTATTTGTtGCTTGATATCATTGAAGCTCTGAaCAaTCTcAACTTAGACGCTCACTCTGTTCAGTCaTCAaCCaTTGACGGCATTCtCACTGTAACCCTTcAGTCTAAGGTCTACTTCTTTCCTAaATtGTTGCaTATTTAGGtCTAAATTTGTTGAAGATATAATTAATAAAGTTGCCCcTCTCTCTAACAGCTTAAGCTTTTAGATAAGTTGGTGGTTAACAATTCAATaTGGTATCAGAGCAGGCAATAGATCCTGTgTTCGAATCTCACTGCCtaCCTGATAATTAAATACTTGCGTGTGTTTAGCCCGCTTATGGGGgAgAGCATGGCTACACGTgAGGGGTCgTGTTGAaGATATAAATAATGAAGTTGTCCCTCTCTTTAACAAcTTAAGCTTTTAGATGAGTTGGTGGTTAACAATTCAATAAAATTTTAGAGACATTAATCACTTTCCTATTTCATAGTTTTGGAAAAAaTGTACTTATACTAGAAATTTTGTGCTACATATGCACTTTCATCGTTTCTGAGATGCTAGTGATTCTTTAAAAACTTGATTTCAGTTTCGAGGAGCAGCAGTTGCATCAGCACGGATGATCAAACAAGCGCTTTCAACTGTTGCCGGAAAGTGTTGATAAACTCAAGTTTGCATATTAATGACTTCTGTCTTAGGATTTGCTTCAAAACGGTGTTGTGTAATATGTATCATTGCAGTCCTTTCAGTCTTTGAGTTTTCTGGTCTCTTAACAATCACTTCTCATGGTTTACTGTCTAACCTAGGAGCATAAATGTGGATGTAATTTGGCTACTATCTGCACCATGTTTCAGTTTCATGTCAATGAACTACTTCGATTTTCAGCCTTGCTAGTATTTCAATCTACTCTGGCTGATGCAATAGGAGTTCTTTGGCACATGAAGAAAGGGAAATGGTTAAGGAGAGCTAAATCTATTATGATTGTAGTTTTACAATTATGACTTCAGTTCAAACAGTTACTGAGTCTCCATTAAATTGAGATGGTAATTAACACCCTGGGGATGATGCAAGCTGGTAGTCACCTGCCAACCATGGGTCTCAGGTAGTCATGGGTTTGACGTCCCCAAGTACTAGACGGCGAAGTTAGTTCTTCAAATTAGTCGGCCCACTGAGCCGCATATCGAGTTATAAAACAAATTGAGATGGTAATTTGAGTTGATAACCAATGCCTAAATCTGTCCTCAAGATTCTGAGCATTAAGACGCAACTTTGTACTGTTAGCAAGATTTGGCATGCATTAGGAGTTAAACATTCAAGTTGCTTTCCACACCCCCTACCTCTTTCTGAATTAACTATTTGATCCATCATTTACTAACTATTTTAACCTTTGGGAGTTCTTGAAATTAATACCCGAGTTTCCACTATTGACTAAACGGATTTTCGGTATTTGCTTCAAAAaAaTTCACTGTACAAGACAAGTCATTTCTCCTAACTAAATGGACTAAATGGTGTTTGTAGTGGGAACGAGTCGTCTTTTGTCATCAGGCTGAAGATAAAAAATATATATATATAGCGTGAACAAATCACCCTTAGTTGGGAAATCTTTAAGCTGAAAAAaAACTGCATCATGCATAACAGGTGCAAACAGCTTATTGTGATTTCTACTAATTGTTCAAGCCATTATAGTGTCTTCATAAAAGGGCACCAACTTTTCATCTTGTCCACCAAAGCTATTAGCATGTGATATGTGCTTAAAGTTCCACAGCATAGCTGTGTTTGGCTAATCCCTTGCAGGGACATGTTTTCATGCATTTTACACAGATTAAAGTGGGCAACGTACCTTATTAGGGACTAAAAAAaGAAAaTGTTGCTACCTATATATAGTAGATTAAATCAAAAGTGGGAACATACCTTATTAGACATGATTATGATGGAATTTGGAATTGCACTGTACCAAAGTTTTGTTTCCAACTACTACCAACCGGTCAAAGCCTCCCAGTCTCAATTGCTGCATTTTtCAAATTACTtGTTTtCCCCATCAATTACCCACATACATATAACTCACCACATATTCACTCAATTGTGTTTGGATCATGAATTtCCGGCAAAATATGAAAAGCGAAGAAAATAACGAAGTGAATAAGCTAAATTTATTTCGTCAACTACCACTATTATACCTTTCGTAGATCCATAATCCAAACACAGCCAAAGTATGACAACCCAGTGATTCCAAGAAACCACAAAAGAGTCTTATTCTACAACAGATGATTGATAAGAACTTCTTCTACCATGAACAATGGCTTCTATTTACATCCACTACATTTGAAATCTAATACCAGCAAAATCTCTgACAGTAAAGCCAATTTTCTCAAACTTTCCCTTCTCATTGCTCTCTCTGAATCTCAAATAATCCGAGCAAACGAATGGCTTGTAAATCCTCGAATTTCCCTCTCCTACCACTTCATCAGGTGCCAAGATCACCTTATCATTCTCAAAGCACCAAAAGAAAGCAAGCGAAAACCGGTTCAGTGGTTTTTtCAAAACAACTCTGTGTTCTGATGACCTTAACTTCTCATTGCTCCAAGCTTGAAGCAAATCACCAATATTGACcACAAGGGTCCCCTCACATGGACTAaTGTCAATCCAATGGCCTTGATTTGATCTCACCTGGAGCCCACCCATTTCATCTTGATACAAAATTGTTAGACAACTCATATCAGTATGCATCCCAAGACCCTCAACCTCTTCTTCCAAACTCTTTGGAGCTGAGTAGTTAATTATCCTCAAATAACCATGGCAGTTTTTGTACTCAGAATCATAATACTTTCTTTCAACATCATCACCCAAGGTCATCAACACAATCTTCACAATTTTCTTCGATAAATCTGTCATTGCGCTTCCATATtGTCGCAATATTTCACTAACCAAAAGAACAAAAAAaGGAAGAAAGAAAACTGTCAACGAAATTTGAACGGAAACAGATGAAGGGATGTTAGGTTGGACTTTTCAATTTATGCACAAAGTGTCTGCTCTTTGAAACTTTTACCACTGAAATAGCAGCCCCTTGTACCCTTCAAATATTGAGGGACTCAAGGGACGTTAGGTTGGACTTTTCAGAAGCTAAGAAATATAATTGACGAGCTGAAAAAACTTCGCTGAAGACGAAAAGATTCTGAACAATTTTGTACTTAAACATCCCCAAAGGAGTTTTAGCCAGATAAGGTGAGTACGTAGATAGACAATAGCATTACCTGAACTCAGAATAGTGCTTGTCAAAGAGAACATCTGCTGAACTCTGAGCAGAGCCGGTGAAGTCTGGTCCAGAAACTCGGAGGCTCTCAAAAAAaGGAGATGCTATGTAATGAGGAGTGTAGGTTTTGATAGACGAGAAAGGACCGAGTTTGATTTtCGTCTCATCAGGAAGACTGAAGAGGAATTCTGAAAGTAAGCGGATTTTATCATAAAGGTCTTTCGAAATCCCATGATTGGTGATGTGGAAGAAACCCAACTCTTTGCAGGCTTCAGAAaGAGAGGACAAAGAACTAGAACATGGCTGCAATGGCTGAGAGATGTCTAaTATGGGtAGTTGTATGCacaCCTTGGACACCATTTTctATATACAGGAAAGGCTTGGTTTgTCCAtcTAGGTTGGGGCTATTATATATAAATCAACTGATAAAACAACTTTtGACACTACCATAATATtATtATACAGAGGCCAGAGTAtGGTAGAAaTAGAGATTCACTGTATagCATAGTTGCTTAGGAAGTCTTtGTAAaCAAACTTTCATGAGACTTGTGAGATTAAACATTAAAGGCGTAATTATCTTCGCTTGCCATAAAATGGTATAATAGTAGCCAATTTTTCGCCATTTGTACCTCAACCGTTCcTACTTTCGTTTtCAACTAGCGTACATTCAAAGAGGAAAGAAGAGGGGAAAGGCATCCCATTATTGGTGATGTGGAAGAAACCCAATTCTTTGCAGGCTTCAGAAAGAGAGGACAAAGAACTAGAAGATGGTTACAATGGCTGAGAGATGTCTATTATGGGTTAgTTGTATGCATGCCTTGGACAACATTTtCCTATGTACaGAAAACGCTTGGTTtGTCCACCTAGGTTtGGgCCATCATATATCCTAtATGGCTATTCTAGTCTTTtGAAAATGAAAGAGagaTATATGAaTGTcTATGAATCAACTGTCCATTTAACCCTcTTTTTtAGAACCctATGATCATTCAAATACtGTCATCAAGCATGTACAGTTCagTAACTCCCAAACTACCATTAAAACTATAAGGACCTTTAGGTAGTGTAACCGACAAGGATTGAGCTCTCTTTCGATGCTCTCAGATTGATCTAGTgaGGAATAGAATTGAGTAtGATGTACATTTCATATGTGCTATcGATATCCTCATCATATATGCAAAAAATACTGtATGTACGACGATAATGTTGtACGTTGATGTCTATtGAGCTTTTACCaTCATAAGAGTAcAATTTTACATGAAATATCCTGTGATAAACAACTTTTGACAGTACCATAATATTATTAATAGAGGtCAGAGTATGGTAGAATAGGGATTCACCGTATAGCATAGTTGCTTAGGAATTCTTTGCAAACAAAACTTTCATAAGAcTtGTGAGACTGAACATCAAAGGCGTAATTGTCTTCACGTGCGATAACAAGGGTATAAAAGTaGCCAAaTTTtCGCTGTTTGTACCtcAACCTTTTCTACTTtCCTTTtCAACTGGTgtACATTCAAAGCGGAAAAAAGaGAGGAAAGGCATCCAATGCAAAATaCCCGAAGTTATCaTTTTTCAagCAATGCACAGctCTAAACTGAAGTATTAACTTTGCCTAGTCCATGTCAAAACATGAACCAACCCAACACCCCTtATtcGGTGCAGCTTAGGTAATTATTGAGCCCAGAAGAAAGCTAaTGGACGTTAGAGTCCCCCACAAAAAGGCAGATAAGGGGGGAAAAAGAAAACAACATTGGAGACTAAaCAAATCTCAATGGGTAACACTCTTATTAGACAAAACTGCTGTAAAAAGTTCATTAATGAGTTCTGGAACATTtACACACCAACGCCACAACAACCCGAATTACCCGGTTTTCTAATGGAAACAGACAGAGTAATGATTTGATGCATTTTTtGAtGAAAAaTtCTACAATCAGCCCAATGGGCTATCGTTAAGGTTTTAAAAGTGGGGACTTTTCCGCGTAAGAATCTTCAAAAAGTGGGAACTCTTGCACGCAAGAGTTAACTCTTTCATGCAAAGGTCTTACCTTATTGTCAATCTAGTGGGGACAGAAGGATTGTTTTTTTtATTtGCGGCCACTACACGTTACTCGGCCAAATaTATAGGAAAATATGACCTGCTCCTTTTCTAAATGCAGGGCACATTTTTtGTTCTGAAAAGGAATCTGcGGAGGTAGGTGATTTGGCTCcCATATAACTGTGTAGGAAAGGTGGTTGACAGGTTGAGTAGTTtGTATCTTTCTTCCGGTATTGAGTACCGACTCAACACGGGAAAACTTAAGAcgTCTAGACATAGTAACCATTGACAAACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGACCGTTGGTgTtGTTGGTTCGAAAAaCCGCACCCCCTTAAATCTTGGAAGgAGTTCAGTGAATCATATtGTTAGCCGTTTTGGAAGAGTGTTTGAAGcGTTGGAAATTTACGAAATTTCGGGTGAATAAAATCGGGACATTTGGAGGGTCTCACGAGAAAACTGAAAAATCCTCTCCAGATTCCTTGAATCTGGACAAGGCAAAAGTTATGGGGCTGGTCCCATGATGTGTGGTAACGTCTCAAAATTATTTCAGCGGTTTCAAAATTAATTCTCACCTAGCAACTCAAGGCTTTCTCCTTGTGTATATTATGGAATCCCGTTTCTATAGCCTGGCCCAGGACCATAAATTCGAATTCCATAAGACATATTTTGTTTTTCGTTCATTATGTATTTATGTGAGAGACACACGCAAGTTTAGTTCGCCACGGCTGTTTTCATTGGTGCACTGTGATTTTAGCCGTTTCATTCTGGGATGTAGACTTGTTGGGTATGCAGACAACTTTCAACAATAGCCCGGGGTGAAGGAGGCAACGCCACCACGGCAGTTGGCACAAAAGTTTGTGCCGCAATCCATGTGCAACCCTAAGAGTAcACGTATTGCGGCGAAAACGGTATTTTGTAATTGGGCTAGAGCAATTTCGGCCACCTATTGCAATTGCTTTGTAATTAGGATTCGAGTGTTTTCAGATTAAACACGAGATGAgAaGAGAACTTTTTtGAGAGAGAAGAGAGGACTTGTAGAGTTATACTCCATATTAGAACCCcAAAACGGGTAAGGGTTTGGGTAAAGAGTAAAGATTGATTTTCGATAATTTtACCCAAATTTtGAGTTTGGATGTTAAAATGGGTCAAAATTGGAGATGCTTTTAGAGATACTGGATTTTGATTTTTAAATTTATCACTTGTTGTTTCTCCACAAGGAATTGATTTTGCACTCAATTTTTTTttATAGATGCaCTCCAAAACTTGTCTTTTATTATTtCAAaTTACATGTAACAAATTTtCAAAGCACACTTAAAacAaGTGTACTAGTTCTTTTGTTcGTTTAAAaGGgAAAAAAAAAaTaCTACAGTAATATTCAATGGAATaTTAGTCAATTCGGTTTCCCACCGGTGCAAACCAGTATTTGAGCCGGAACCTACTGCTAAACACGCTTGAGAGATCAACGACGTCGGGTGGGTTAGGGAAAAAAAAAaCCTCCAAtACGTCGCAGAAGGGACCTTGTTTGAATTACGGCGGGACTTGCAACTCTCTCTCTCTCTCATGAGCTTTCTCTGTCGTCTATCGTTTTCCTCTATATATACATAGATGTGCCCTCTTGTTCGGAAAGCATACAACCTGTTCGAAGAAATAgCCCAAAGGTGCCCTTTTGTGCGAAACTCTGTACGTTGTTTATCAAACTGTGCGTTGAGTCAAACCCATTCCTATGAAGACCCAATTCAATTTTCTTATGAAACTCAAAATGTAATATCTTGGACCTCAAAAATCTCCGCTTTAGTGAGGAGAGAGAAGCCCCGAGAGGCCATTGGGTTGTTCAAAACCATGCTTGTGAAAGAGCAAAGGCCAAATTATGTGACAATTCTGAGCATCATACGCGCTGTTGGCAGTTTGGGTTCTGAAAATATGACCCAGGCAATTCATGGGTTGTTGTTCAAAATGGGGTTTGAATCAGAAATTCCAGTCGTAACCGCCCTTTTGGGTGTTTACTCTATGTGGGATGTTGAAGTTGTATGGAAGCTGTTTGATCAAATACCCAATAGGGATATGGTTTtATGGAATGCAATGGTTTCGGTGTGCGCgAaGAGCGGGCAATACAGGGAGGCGTTTGAGATTTTTAGAGAAATGCAGTACGATGGTGTTAAACCAAAAGATGCCAGCATTGTAAGCATACTGCCTGCTTGTGCTGCTCTTGCTAATTTGTCCTTGGGCAGACAAATACATGGTTTCTCTGTAAGAAGTTTATGTTGTTCACTTACAAATGTTCAAAACTCACTTGTAGATATGTATGCGAAATGTGGAAATCTTGAGACCTCTATTCAAATATTCAATAGAATTGAAAAGAAGGATCTTGTTTCTTGGAGGACCATGCTTCATGGGTGTATCGCCAATGATTGCCCTCGAACGGCACTGAATATGGTCTCTAGAATGCAATCTAATTGCTTTAGACCAGATGAACTTTTGATCCTTGATGCAATTGGAGTCTCTACACAAGAAGAAAAACTTAGTTTTGGGCTGGGATTTCATGGTTATATCGTAAAAaGTGGTTTCTTGGATTTTGTCTCGGTTGTGACTGCACTTCTCCAAaTGTATGCTAGGTTTGGTGAATTTGGTTCAGCTGGGGTTTTATTTAATCATATCACTCAGAAAGATGTTATTGCGTGGAGTTCCATGgTATCGGCATATGCGCAGGGTGGACATCCTTtGAATGCTTTAGAAACTTTCAAAAAGATGCAATCAACAGATGAGAAGCCGAATGAGATCACTCTAGTCAGTGTATTACAAGCTTGCTCTtCTTTGGGAGTCCAAGAACTTGCGGAGAGTATCCATGCGCATGTGACTAAAGCTGGCTACTCTTCCAACACAATATTACTACCAGCATTAATTGATATATATTGCAAATTCGGAAGGATAAGGCAAGGAAAGGCACTTTTTGATGAAATTCTTATCAAAGATCTTGTCTGTTGGAGCTCAATGATCAATGGTTATGCGATAAATGGGTGCGGAATTGAGGCTCTGGAGACCTTTTCAGAAATGTTGAAGTTTGGAATAAAGCCCAATGAAGTTGTCTTCATTTCTCTTCTATCTGCTTGCAGTCATTGTGGGCTAGAAAATGAAGGCTGGACATGGTTTTATGCAATGGAGAAGGTATATGGTATTACTCCAAAACTTGCACActATGCTTGCATGGTGGATCTGCTTAGTCGTCAAGGACATgTAGAAGAAGCCCTTGAATTTGTGAAAAGTATGCCTATTGAGCCTGATAAAaGAATTTGGGGTGCTTTACTTGCTGGTTGTAGATCAATTCATGGCtCTATTGAGATAGCAGAATTTGCGGTGGAACGGCTCATTGATTTGGACCCGGAAAaTACTAGCTGTTACGTGGTTTTGTCAAATTTGTATGCAGAGCAGTGTAGATGGGAAGACGTGGAGAGACTGCGGGAACTGGTAGACAATAAGGGATTGAGGAAGGAAATAGGATATAGCATTGTTGAAGCCAATTCATAGCATGCATATTGTGAGATTGGTTAGCTGTGTTCTCTCAACAGGTTTCTTCTTGTTTTTtGTTtGACTATCTGTAGAATGCTGAAAATAAGTTGCTGGATATATCTCTTACTCAGAGATCATACATCCATTTTCAATCTCACCTTTTGGTTTGTCCGTACATCACACTACATACCTTAGACAGAACTTCTTTGAATTTTCCAGTAACGTGAATTTAAATAGGCGTCTGTGAATTTAAATAGCcaTCTATGTATTTATTGTTGCGGAGTTACATTTAGTAGCGGCTTAAGTAAAGAATTTATTGCTCATTGAAAGCAGTACTGTATGCTTCCTTTTATATCCTCCATTAATGCGTATTTCACTAAGTTAACTTTCCAGACCCTTGTTACTAGTCCAATAAATTCTCGAGATCTTTTCCATATAAGCTTTGATTTATATCCCGTTCTGATGAAAGAAGGAATCCTTGAAGTACTTCTTGTCAGCGCTAAAGACATTAGAAACACCAATGTTATCGGTACTCTGTCTCTCTCgCAGCTAATGTCTAAagAGGCCATGTGTATATAATATTTACACGGATTATAGAAAGCACTTTGTTTCTTTGTGTGGCATTTTTTGCTTTCTCTTTTGCATTAATTGGAGCTTGTTAATTGTTCGAACTCAACCTCGTGTTTAACAGGGAGACCAGCATATTATGTGCTCATTGAATGTGGAACTCAAGTGGAAAGAACCAAAACTACAACAGGTCAAATTCTTGGTGATAGTTCATCCATCTTCCTAGCTCTCATCCTAATATGCTTTCCCTAACCTAATTACAGAGTACATTGGTGCAGGTAATCACAAAGAAATATGCTGGAATGAAAAGTTTGCGTTTGAATTTCCCTCTTTCGAATGGGAAAACTTGACTCACCTCAAATTTAAAGTCATGGCTGAGGAATATTTGTTTGAAGGAGGATTTGTTGGTGAaaCCATGTAAGCAGTTTTAACTCAAAACATTATTCTTGTTCAGTTTTCTTTGTTAATTACCAGCCATATAATGCTTTCATTTTCTTTGTTAAACAAACAATTTTGTACTTTCTGCTAGGaTAAATaTCaCAAATCTTCTAGAGCTTCTTTTAtGTcAAACCCaGCAAGGAACTTGCTCTCTCAAACCATCTTCTACCCTAGTAGTATTGAATATTGATCACACGAAGTTAAGAAAAAaGGGTTTAATTTCTGCATtAATTTTTtGTTTTTtGAAATCTTGTGTATGCATTAAGACATTTACTTTGGCATTGGAAACCTTAtGAGTTATGAAGGAATGAATATGTCTTAGAGGAACCGTTGTCTCTCCCTCAATTTCTGTATTTCTATAGGTTAAACTCCTCGTGATGATTATGAACAATGTGATTTGAAGCTTTGAGAGCTTTTAACTAGTAAAGCAGTTCATGAAATAATGGAAGTACTAGCTATAATATCTCcTCATTTGCGTATACATACATTTTGTTTTtGTTGAAATAATTTCTGTATCCCAGATTCtcGGGgCAAATTAACTATCTAGATATTG


>CF810540 Length = 633 

Score = 69.9 bits (35), Expect = 4e-11
Identities = 66/75 (88%), Gaps = 1/75 (1%)
Strand = Plus / Plus


Query: 5863 tgttgaagatataattaataaagttgcccctctctctaacagcttaagcttttagataag 5922 

           |||| ||||||||| ||||||||||  |||| | |||||||| |||||||||||||| ||

Sbjct: 392 tgttaaagatataaataataaagttatccctttatctaacag-ttaagcttttagatgag 450


Query: 5923 ttggtggttaacaat 5937 

           ||| |||||||||||

Sbjct: 451 ttgatggttaacaat 465


11B) MYCA1 scaffold 00554 Submitted from SSR primer area

GAAAACTACtAaGAAACATTGTCACAAATGCAGAGGAACACAAACTcTTTCCAAACCAAATGAAGAAACTGAGTTACGTACGCCCAATGTAAACATTATGACTTTTTACtaCTAACCCTAGTGCAAATTATTGCACTATTGTTGCATTATGAAATTCTAAACTATCACAAGAATAATGAAGGGGTTTtATAGGCTAACCcTAacAACCTTAGCAAAATCCAAATAACCCAAAAGGACTAAAATACCCTGAACGTAAAAcaaaaaTAACGATAACCTTACAGAAACCcTAATGACACAAAAAGACTAAACTACCAtGAACATAAACAATAATCTGAAACCCTAAAttACACTCCCCCCCCcTCCTCcTtCAAACGCAGGATGCGAAGCCGGAGAGCATTGTGAGTTTGCCAAGGGATTAACAACAGCGAATCAACTGCTGTGATGGTGCTGAACAATGCAAAGCaGTACAAAACTAGAATtTGCGAATAGGAAGAAGAACTGATGCCGAATAGATGCAAATAGGGGAAACCAATGCCAAACAGATACCAACCAGATGTCAAATTGCtGATGCAAACAATACCAAGAGAGAGAAGAGAACAGAAgCAAAGTGTCAGATCAATTAAGATGCAAAGATCGACAGAGgTGTAGGGAGGAAGAACAGATGCAgAGAGATGCAAAAACTGATGCAGATTTAAGAGGTTCAAAAGTTTAGAGGTTCAAAGGTCCAACATAGCAGAATATTGCAGAATCAGAAGCCCAAGTAAGAGCGCAAAATACCATAGTGGTTGTAGAAGcGGAGGTCGATATAATGTGCACCAGTCAATCGAAAGAAACCCACTCAATTGATGCAATCTGTCGATTCCAAGTGCATTAGGGAgaGACCCAGTCAACGTCGTGGACCAACCCAATCAGTTTAGAAGTCAATCAGAAgTtACCCAGTAAATTTAGGTAGAACTACCCAAAAGATTATGAACATGATCTGAAGTCATCGTGGCCaGaCTGGAGATTGGTTCGCCCAAGGTCGTCGGAGCAAGTCAcTGGGTGGgAGGTGGCTGCTGCTGCTATTGTGTTGTTGTAcGTGATGTTGCTGCTGGTGCGATGAACAGTTCATgTGAATGCCCCGcGAATGGTGGCAAGCCTTAGGcTgCCcGCCGGCGAGtGAATATCCGACAtgCCTAGATCTTATTGCTCTGATACCATGTGACTTTGCAAAGAaGGAAAACAAAACTAACGTATTATtGCaTTATGAAAGTCTAAACTATTACAAGAATAATGAAGGGTTTtATAGTCTAACCcTAAAAaCCTTAGCAAAaCccTAATagCCCAAAaGAAAaTACCTGGACATAAAAGATAAATAAcGATAATCTTActGAAACCCTAATGACACAAAAAGACTAAACTAcAATGAAAATAAACAATAAACAAAAAGACTAAATCAAAGGTTGGTAACTTTGCATGTAAGGGACAACCCACAACTCTACTAAAAAaGGGGGACAAACCATAGATTTCTTTTCAATAACAGCTTtCTTGGAGAGAGAGAGAGAGAaGCATACcTGTAATAAtCCTTTTAGGTCCAAGCTCCCTCACAAAATCAAGTAGTCTAGACAATGCATCAGGAGTACGAGCATAATCCAcAATCACCCCAAATGCTTGTCCCTCATCTATCAACTCACACCTACCAGGAACTGCATCAACCTCTtCAATCCcTCTAACAATATCCTCCAAAGGTGCTCCAACCGCAATTCCAACTGCAaCAGCTGCAAGAATGTTGTATACATTATGTCTGCCAAGCAACCCCGAAGAAATCTCCAATAAACCGTCGGGCGTTTTTACCAAGACCTGGGTCTCAAATAGAGATAGTTCAAATTTCAAAGAATGAACGTCAGCatCTTTATTCTCCACTGCATACGTCACAACAGGCACATCTGGGTTgCCTTGTGCAATAAAGGAaGCTGCATTCGGATCATCAAaGTTGACGATTTTCCTGTGTCTTTctGGGTCCACCATTCTTGAAAACaGCTTGACCTTAGCATCCCTATaCTCTTCCTCagaCCcTTCAAAaTCGTAACGgtCCCtGTCAAATTTGTGAAAACGgCAATGTCAAAGTCAACCTCATTGCACCTCCCTAATGCTAGTCCATGTGACGAAGCCTCCATCACAACTGCTTCTGTCCCATTATGCACCATTTTCGCCagCAACTTCTGAACCAAAACAGCATCTGGAGtcGTACTTGGCGACTGCAACTTaTTATCTCCATGAATATAATACGCCACGGAGCTGAACATCCCAGTTCTCAATCCCATGGCTTCATACAACCCTTTAATCAAATACGCGGTGGTAGTCTTCCCATTTGTACCAGTAATCCCTATCACGGACATTTTCGTAgAGGGGTAaCCAAAAAAAGCTGCCGCCAATGCTGGgAGCACCGAACTTGTGTCTTCCACAATAACCACAGCCTTACAACCTAATGTCTCCTCTAAATCTATCTCTTTACTAGTTACCACAGCAACTGCACCTCTCTTGTATGCCTCCGTCAGATACAAATGCCCATCAGTTTCCCTtCcGACACAACACACAAaCAAATCACCAGCCTCAACAAACCTCGAATCATGTTGAATCCCACTAATCTCTACCTCTAAATTACCATAAACTGAAACAGGTACTACTTTACTCTCATCTAAAAGCTCAGCTAATGTCATATAAAACGTGGGGTCAACAATCCTAAACTTGTGACTTTTATCAATCTTATCGAAATCAATATCGAACGATGAATCAGAACTCAAATCAGACTTaCCCAAATCAGAACTCACCTCATTCTCCAATTTCTCACCCTCTTCATCATCACCCGCATCTGCCGGAATCACAGTGAGCCCTCGCAGCTCCTCAATTTCCTCCAAATCAACCACTTCTTCAGGCTCCAGCTCATCAATACCCTCATCCACTTCCTCAACCTTCTTCTCTTTCCTAGGGTTAGGTTTCAACAAaCCCTTTTCAACAAACTCTTTCCTTGACAAAGCAATAGCCTTATCAATATCAGCAAACACATCATCCCCCGAATCTGTTGGAAAAATATTTTAGAATTTCTGTAAACcAAAAAAaCCAGGGACAACAaTACAActATAGCTACCATAAACATTTGTGGCTACGGGAGTTACATTTGAAATTTGTAACTCTTTTACAACTTTCAATTAGCAAATCATTACAATTAAGTACAATTACAAAGCTATCACTGTTGTAAAATCAAATAATAGGTACCATGAAAATAAGAGAGAGTTCACTCTTGCCTTGGGcgAGGATAACTTTatCCACATTCGGCGGGgTTTTGTGGGTTCCGATTCGGTAATCAGAACCATTcATTTCGTATGACTTGATTTATCCACATTCGgCGGGGTTTTgTGGGTTCcGATTCgTTAATCAGAACCATTTATTTCGTATTACTTGATTTATTAGGAGACcaTGTGAAAaTTCAGCTTAATCATAtAGTTGTAAGCACTCAaTCCAAAATTttAaTTTTtCTCCATCCagTTCTAAAGCTAaGGAATTaGACTTtCTATGCTCAaTATctAATTGATCTAAATTTTTTTGCGTTGTCTCCTAACAAATCAATTCATCCAAAaTAAGCAGTTCCAATTCCCATGGGTAAAATTACACTAGCTCTGTTGAACAGAATTGCTCGCTACAAACAATCAAATAAAATTCAAATCACAAAAAATCTAACAGAATCACCACCTCGGTCTTCGAACCCGGGATCGTCGGGTGCGTCCTCCTCGTCGGCAATTGCATTGAGAAAAATTGATTGCTCCTTTTCAGACCGCTCTTCTTGGATCTTTCTTGCTTTATCAGCATGGCGCTTGACTTGCTGGAACCTGGATACCCCGTGCATGGAGTCTTCTTCCGCTTCGGGCGGGTCATCGTCGGATGGGTCCGGTTGGAATACGCCGTcGGGgCCGATTGCGGCAGGGGGTCGGAGTTGGAGTTGGGGTTTtAGGgTTtGGTGAGgAaGAGaGAGGAaaGGgAaGGgTTtGAaTtGGGgAAAAaaTGTtGGATTTTtCAGAGAGGGGGTGAAGAGTGGgAAGGAGATgAAGGTGGGTGGCAATGGCATTGTAGAGAGAGATTAGAGAGAGAGAGAGAgNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAAACTCAATTCTTGAGGCCGCAAACaCAATTCATGAGGCCTCGAACTCAGACTTTGTGGCCGAAaTGCTTTGGCTAAAAAAaaaCATTTTtttCTTtGGTCTTCATTAAAATAAAAaTTTGCATtcGTTAGTTTTGCGACGATTTTTGTGGTCTATTGGTTCGTtCcGACGAGATGAATCTAAAgAGtaTAAAAttAAAATTTTtaaagaTTTCACTAAAGACAAAAaTAATCTAGATTTTTgTgATTtATTAGATTgTTTTtGTCTTTATTCaCAAAAATTTAAGTTtCGTTCATAATTTTAtACTTTTTtAGATTCCTCTCGtGgAGAGAATCCAATAAATCACAAAAAAaTCGATGCAAAACTAGCAAAAACAAATTtCTTTTGAaTTAAAAAAAAaCATTTtAGTTTTTTtAGCCAAAGCAAGTGAACGAAACTTGAATCTTGTGACCTGAAACTcccACCTTGTGACCACTAACTCGAACCTTTTGGCCTCAAACTCCAACCCTTtGGGCCGACAAAATGTtATTtCTCATCTTAAAAActtAGGCCTaTTTTGGCCAAAGTAGAAAATCAAGCTTTtCcTTTtAgCTTTTTTTTTTttGGgAAAAAAaCTCAAAGGTAAAaGGTGGATtGTTTtGGCcTTTTTTttGTGCTTTTTGCTTTTCACCTTTCAAATTGTAATtGTTTTTTtATtACTTTTtGCTTATtGTAAaGTTACTTCTTTAAGCCAAATTCTATGACTTTtCgAaTCtCAAAAAAGCAAAAGGCTTGCTTTTCTAGTTTGGCCAAAATAAGCcTTtATCTCCATAAtttgttGACTACTTTGTtATTCATGATTTTTAaTCTTTAGtgATTTCTGTCAAATATTTtATTTTTTCCCTTTTTAGATGCATTCATACCTCTgTTTtAAAAAATATAAAAAaaaTTATGATATTAAGAAAAAaGACTAAAAaCgAAAAaTAGGCAACTATATACAAGCCTTTTtAAtCtAaaTAAAAAaCTCATCCAAaTAAATGCTACTCTAACTTGTTTTtCTCAACACTTTTGTCAACAACGTTTAAaTAATCTTGTGAAATGGAAATGAAAACTATTTTAACGCTCCTTTTTTTCTTTCTATCTATTGgCACTCAATTTCTTATCTTTTATtgCGTGACAAAaCTAAACTATCCTTGgTtCTTATCTCTTGAGTCAATTTTTtCATATAAAAGGTAaTTCGAtACTtCCGCATGAATAAAAACCAAAAAaTGGGTGCCAATGGATAAAAAaTTaGCGTTAAAatCATTTCCCAAAATAAAaaTaGTTgCGTCAAGGTTCTAAAtaCCATAtCGTTTTTTCtAGTTGTACAATATGAACCAGtNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTGCACTATGCTGCACCCTCCTCagATATTTTTGGTCagCTTCCCGCCAAACCCACTAGgCTAAAACCCTAGTTGCCTTTTCCCCCACTCCAaAACCCTAGTTCtCTCTCTCTCCCTCCGCCACCAGTTCCCCACCCcACCACAACCAACCATGGACCCTCAGTCcGTTCAGACAAACCTAGTCCTCATCCTAGACTTCGGTtCTCAGTACACCCACCTCATCACgCGACGCATCCGATCCCTCTCCGTCTTCTCCCTCTGCATCTCAGGCACCTCCCCTCTCAAATCCATCACAGACCtAAaCCCAAGTGTCATAATCCTCTCCGGTGGTCCCCACTCGGTtCATgCGCCCAACGCGCCGTGTTTCCCtGATGGGTTCATAGAGTATGTGGAGTCTAAGGGCGTGTTTGTTTtGGGCATATGTTATGGGTTGCAGCTGATTGTGGAGAGGCTGGGTGGGTGTGTtGGGgTtGGCGAGAAGCAGGAGTATGGGAGGATGGAGATTGAGGTGGAGAAGGCTTGTGGTTTGTTTGGTGGGTCAAAGAAAGTTGGTGATAAGCAGGTTGTTTGGATGAGTCACGGGGATGAGGCTGTGAAGTTGCCTGATGGGTTTGAAGTGGTGGCGCGGAGTCAGCAGGGGtcGGTGGCGGCTGTGGAGTGTCCTGGGAGAAGGTTTTATGGCCTTCAGTATCATCCGGAGGTAATTGGGTATCTTCATTCATTTCATTCTTGGCCTTGTGATACGCTGCCCTGTTTTAATTGTCTTTGTAATTTGGTtCTTTGGCTGGCTTTGTTTGGTTCTTTACTAGTGGTTATTGTTTGTACGCGAAGTGCTTACTTGCTGATATCATCTTCATGTTGAAGTGGTAAtAATGAATGAAAAATGGAATGAATTGACCCAATATCTGAAAAGTTATCGTCTTTAGTGGTACTGTGGTAGTGGCCTATAAAACTGTTAGCGAcACTCCCTCGtGATGTATCCTGCTCAGGCCcAGATCCTTAGCAAGAATGAGTCAATGACCATTGATCCCTTTtCAAGCACTTAAGCtgAaCTTCCACATGgTACCATAAAATCCCTGAATCCTCTCAGCACAAGCACTGATGATCTCTCTGTTTGAGTTTCACATCACTAGCCCAACCCCCAGAAAACcATCCTTATAGTTATAGTAGCCCCGTCAAACTGAATTGTGAGCTCACCTTCATTTGGAGCTTTCCATGAGCCAGCAAGATGTTGGGATAGTACTACAGTCTTTGGAAATACAATGGGAGTTCTCTCAGGTAGCTAGCTGCATATTCtGCAAGATCACTGGCTCCCCTGTTTGTCCCtccAAAAAaTGTAATTGCCTATTTAACCAAATTACCCAAGCCACAATTAGAAagAGATCTATTATTtGTTGGAACCAACCCCCTCAATCTGCATAGCCCAGAGATGCCCCTTTCTTCAAGCACATGCAAATCTTTGAATCGATAACCTTCCGTAAcATTCTTTGCTTACTCACaCtCTTTTAGACAACGAATGATGGTCTCCGGTGCGGCGCTGTCCCTCCACACAGGCTACAACAATCCTTAATATTAACCATTTTTTtGACAAGCTTATGGTTCATTGGGAAAATCCCCAAACACAtCTTTCAcacaatgaCACAAACAATTTACTTTgTGTTGGgAaTTTTCAGATTCCAGATTTCTCCTAAGATCTcATATTATCCATGTCAGAAGATGAGAGTTGAGAGTgCTGAAAATGTAATGTCCCTTGTGGTGTAACCAGACtCcATGGTGTAAGTTTCTTGTTTAGTGAAGTTCCATGTTTAGTTGTCTTCATTTCCCAGGaTAATAGGGGAATGTTTCATGtCcTATgAGCTTCAAAAGGGaTAAAaGATCACAGCTAAGATGTTCCTTCCAAACCCCAGTATTGTGGTCAACGAGGCCCTTtACTaCCATTCTCTCCGTATCAGGCTTACAaGAGCTTGTAGTTTTAaGGGAGAAGTGCTTGGgATCCATtGGTCTGGCCACACCCTGGTTTtAtATCCcTTTCcGATCCtCcACCTAAGaCCCTTTTTAAGCACCTCCATACCCTCACACAT

>DR067752 Length = 574 

Score = 46.1 bits (23), Expect = 3e-04
Identities = 29/31 (93%)
Strand = Plus / Minus


Query: 459 gaagaactgatgccgaatagatgcaaatagg 489 

          ||||||| ||||||||| |||||||||||||

Sbjct: 353 gaagaacagatgccgaacagatgcaaatagg 323


12) UFGT search with amino acids using tBLASTn SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL


>CV091309 Length = 663 

Score =  124 bits (310), Expect = 1e-30
Identities = 60/60 (100%), Positives = 60/60 (100%)
Frame = +3

Query: 1 SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL 60 

          SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL

Sbjct: 138 SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL 317


>DR067838 Length = 557 

Score =  119 bits (297), Expect = 4e-29
Identities = 58/60 (96%), Positives = 58/60 (96%)
Frame = +1

Query: 1 SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGKEL 60 

          SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKG  L

Sbjct: 352 SIVAGVPIIGRPFFGDHQVDTWMVENVWKIGVRVEGGVFTKSGTMSALELVLSQEKGTGL 531

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