Difference between revisions of "Mastering the Art of NCBI: It's a BLAST"
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The [http://www.ncbi.nlm.nih.gov/ National Center for Biotechnology Information] (NCBI) is an organization founded in 1988 as a national resource available to the public for access to molecular biology information. NCBI creates numerous databases, online tools and research software programs to analyze genomes. The [http://blast.ncbi.nlm.nih.gov/Blast.cgi Basic Local Alignment Search Tool] (BLAST) is an online tool designed to enable users to rapidly search through nucleotide and protein databases.While the website is designed for both novice and veteran users, the task of mastering the tool and the art can be daunting. This website is designed to provide a step-by-step process of how to use BLAST and interpret your results. | The [http://www.ncbi.nlm.nih.gov/ National Center for Biotechnology Information] (NCBI) is an organization founded in 1988 as a national resource available to the public for access to molecular biology information. NCBI creates numerous databases, online tools and research software programs to analyze genomes. The [http://blast.ncbi.nlm.nih.gov/Blast.cgi Basic Local Alignment Search Tool] (BLAST) is an online tool designed to enable users to rapidly search through nucleotide and protein databases.While the website is designed for both novice and veteran users, the task of mastering the tool and the art can be daunting. This website is designed to provide a step-by-step process of how to use BLAST and interpret your results. | ||
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'''How to use Basic BLAST:''' | '''How to use Basic BLAST:''' | ||
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[[Image:BLAST Home.png]] | [[Image:BLAST Home.png]] | ||
| − | [[Image:nucleotide blast.png]] | + | To search for matching nucleotide sequences in the database, choose: [[Image:nucleotide blast.png]] |
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| + | This link will take you to the page shown below. | ||
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| + | [[Image:Nucleotide BLAST Entry.png]] | ||
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| + | In the entry box below '''Enter Query Sequence''' paste the nucleotide sequence (ATCG, etc.) you wish to search for. | ||
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| + | [[Image:Enter Query Sequence.png]] | ||
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Created by Claudia M. Carcelen, 2009. | Created by Claudia M. Carcelen, 2009. | ||
Revision as of 03:21, 8 October 2009
The National Center for Biotechnology Information (NCBI) is an organization founded in 1988 as a national resource available to the public for access to molecular biology information. NCBI creates numerous databases, online tools and research software programs to analyze genomes. The Basic Local Alignment Search Tool (BLAST) is an online tool designed to enable users to rapidly search through nucleotide and protein databases.While the website is designed for both novice and veteran users, the task of mastering the tool and the art can be daunting. This website is designed to provide a step-by-step process of how to use BLAST and interpret your results.
How to use Basic BLAST:
When you get to the main page, you may notice you have a number of options to choose from:
To search for matching nucleotide sequences in the database, choose: 
This link will take you to the page shown below.
In the entry box below Enter Query Sequence paste the nucleotide sequence (ATCG, etc.) you wish to search for.
Created by Claudia M. Carcelen, 2009.
