Difference between revisions of "Melamine iteration"
From GcatWiki
| Line 4: | Line 4: | ||
1) create an effective riboswitch system | 1) create an effective riboswitch system | ||
| − | *retest riboswitches with biosensors | + | *retest riboswitches with biosensors (dissolve ammeline in DMSO) |
*find best riboswitches, test them with the thyA fitness module (insert thyA riboswitch into mutant thyA- cells) | *find best riboswitches, test them with the thyA fitness module (insert thyA riboswitch into mutant thyA- cells) | ||
*use mathematical models to create new riboswitches | *use mathematical models to create new riboswitches | ||
Revision as of 15:44, 22 May 2014
The point of our research is to help prove the concept of Programmed Evolution. We will try to do this by creating a system in E. coli that uses melamine deaminase to create ammeline. The presence of ammeline will be shown by using a riboswitch that will turn on a biosensor module and a fitness module. Our first goal is to create a riboswitch system that works well to show the presence of ammeline and then to work to build a system that efficiently produces ammeline from melamine.
Goals For Summer
1) create an effective riboswitch system
- retest riboswitches with biosensors (dissolve ammeline in DMSO)
- find best riboswitches, test them with the thyA fitness module (insert thyA riboswitch into mutant thyA- cells)
- use mathematical models to create new riboswitches
2) create an efficient melamine deanimase
- place melamine deaminase gene in the correct location in plasmid (MWSU)
- put both the gene and the riboswitch into the plasmid
- vary the promoter, RBS, and compy number to get efficient ammeline production
Eckdahl's riboswitches File:Ammeline Riboswitch Sequences.docx
- note - change to RNA before using
