Difference between revisions of "Melamine iteration"

Revision as of 14:41, 22 May 2014

The point of our research is to help prove the concept of Programmed Evolution. We will try to do this by creating a system in E. coli that uses melamine deaminase to create ammeline. The presence of ammeline will be shown by using a riboswitch that will turn on a biosensor module and a fitness module. Our first goal is to create a riboswitch system that works well to show the presence of ammeline and then to work to build a system that efficiently produces ammeline from melamine.

Goals For Summer

1) create an effective riboswitch system

• retest riboswitches with biosensors
• find best riboswitches, test them with the thyA fitness module (insert thyA riboswitch into mutant thyA- cells)
• use mathematical models to create new riboswitches

2) create an efficient melamine deanimase

• place melamine deaminase gene in the correct location in plasmid (MWSU)
• put both the gene and the riboswitch into the plasmid
• vary the promoter, RBS, and compy number to get efficient ammeline production

MWSU

Davidson

Online UNAcode interface

• note - change to RNA before using