Missouri Western/Davidson iGEM2009

This space will be used starting April, 2009 for brainstorming and a shared whiteboard space.

Davidson Lab Protocols
MWSU Lab Protocols
BioMath Connections Page
GCAT-along Freezer Stocks

We need to learn more about these topics:

Biology-based

1. What is msDNA?
2. How is msDNA normally produced? Olivia
3. How is msDNA stored in E. coli? Olivia
4. How many copies are carried per cell? Alyndria
5. What is the sequence of bacterial reverse transcriptase and can we clone that gene? Shamita
6. Can we redesign the normal msDNA pathway to produce new segments of DNA of our choosing? All
7. Can we use suppressor tRNAs to encode logical operators (suppressor suppressor logic, SSL)? Leland
8. Can we solve a 3-SAT problem with supressor logic?
9. What role can physical modeling of protein structure play in our project? Romina

Math-based

1. What interesting challenges or problems does origami offer?
2. Can we produce a series of increasingly difficult goals that might be possible to produce in the lab?
3. What has been done before and how can we improve upon that?
4. We can perform some pilot experiments using synthesized DNA and later switch to msDNA (maybe).
5. Can we address the Boolean Satisfiability (SAT) problem with a bacterial computer?
6. How has 3SAT been addressed with a DNA computer? Can we use those methods?
7. Can we get bacteria to solve a problem large enough to challenge a person?
8. Can we get bacteria to solve a problem large enough to challenge a computer (probably not, but it is fun to think about)?
9. What are some linear algebra applications for DNA origami?
10. How can we use origami to solve 3-SAT problems?

Behavior-based

1. What constructs are we testing?
2. What school districts do we have access to?
3. Where is the Synthetic Biology page we want high school teachers to use after the survey?
4. Do you need any more input from the veterans before the survey is ready?

Questions from BMC 4/17/09

(In no particular order)

1. Could we have/use multiple synthetic organelles in a cell?
2. What ideas from previous iGEM teams are useful to us?
3. What other math problems (e.g. NP- complete) are accessible to us? Siya Sun
4. What is the relationship between 3-SAT and map coloring? Ashley Schnoor
5. Could we do something with clocks/counting?
6. What role can physical modeling of proteins play in our project? Eric Sawyer
7. What activators are there that turn on a promoter without any help?
8. What other cool reporters are there? (Discrete On/Off or Continuous) Bryce Szczepanik
9. Can we use promoter strength/opposite directions to subtract? Clif Davis
10. Can we use protein interactions to compute? (Post-translation, proteases, quaternary structure) Will Vernon