Difference between revisions of "NHE Mar 8 Notes"
From GcatWiki
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*look at absolute changes in expression levels (genes that go from on to off and off to on) | *look at absolute changes in expression levels (genes that go from on to off and off to on) | ||
| + | |||
| + | ran the normalized plot with raw scores with Q-vals set to 0.01. | ||
| + | |||
| + | 38 potential genes | ||
| + | |||
| + | Potential genes: | ||
| + | *JAGN1 - was one of the POUFs. no expression in non fed. slight increase in fed. secretory protein. necessary for neutrophil survival... | ||
| + | *CYP2J2 - down regulated in Fed | ||
| + | *NDUFB6 - POUF. mitochondrial. upregulated in fed. responsible for NADH oxidation. | ||
| + | |||
| + | Is NAD production significantly upregulated? | ||
Revision as of 19:56, 8 March 2016
POUFS cannot be changed in the files because it breaks code. The gene names have to match exactly how they appears in the reference genome. Made reference excel file instead and uploaded to wiki
Our new direction:
- look at absolute changes in expression levels (genes that go from on to off and off to on)
ran the normalized plot with raw scores with Q-vals set to 0.01.
38 potential genes
Potential genes:
- JAGN1 - was one of the POUFs. no expression in non fed. slight increase in fed. secretory protein. necessary for neutrophil survival...
- CYP2J2 - down regulated in Fed
- NDUFB6 - POUF. mitochondrial. upregulated in fed. responsible for NADH oxidation.
Is NAD production significantly upregulated?
