Difference between revisions of "Nanocircles"
From GcatWiki
| Line 1: | Line 1: | ||
| − | Nanocircles arre small circular single-stranded DNA that can be transcribed by phage and bacterial RNA polymerases. These plasmid-like structures were originally developed by Eric T. Kool's lab. The new technology uses a method called rolling circle transcription (RCT) to encode hammerhead, hairpin and hepatitis delta ribozymes. | + | Nanocircles arre small circular single-stranded DNA that can be transcribed by phage and bacterial RNA polymerases. These plasmid-like structures were originally developed by Eric T. Kool's lab. The new technology uses a method called rolling circle transcription (RCT) [http://www.stanford.edu/group/kool/research.htm animation] to encode hammerhead, hairpin and hepatitis delta ribozymes. |
Figure 1. Structrure of single-stranded DNA nanocircle composed of 63 nucleotides encoding a hammerhead ribozyme and 41 nucleotides of randomized sequences | Figure 1. Structrure of single-stranded DNA nanocircle composed of 63 nucleotides encoding a hammerhead ribozyme and 41 nucleotides of randomized sequences | ||
http://www.pnas.org/content/vol99/issue1/images/medium/pq0125890001.gif | http://www.pnas.org/content/vol99/issue1/images/medium/pq0125890001.gif | ||
Revision as of 07:31, 20 November 2007
Nanocircles arre small circular single-stranded DNA that can be transcribed by phage and bacterial RNA polymerases. These plasmid-like structures were originally developed by Eric T. Kool's lab. The new technology uses a method called rolling circle transcription (RCT) animation to encode hammerhead, hairpin and hepatitis delta ribozymes.
Figure 1. Structrure of single-stranded DNA nanocircle composed of 63 nucleotides encoding a hammerhead ribozyme and 41 nucleotides of randomized sequences
http://www.pnas.org/content/vol99/issue1/images/medium/pq0125890001.gif
