Difference between revisions of "Nanocircles"
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| − | Nanocircles | + | Nanocircles are small circular single-stranded DNA that can be transcribed by phage and bacterial RNA polymerases. These plasmid-like structures were originally developed by Eric T. Kool's lab. The new technology uses a method called rolling circle transcription (RCT) to encode hammerhead, hairpin and hepatitis delta ribozymes. |
| − | [http://www.stanford.edu/group/kool/research.htm Rolling Circle Animation] Click on Rolling Circles & Artificial | + | [http://www.stanford.edu/group/kool/research.htm Rolling Circle Animation] Click on Rolling Circles & Artificial Telomeres |
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| + | == Experimental Design == | ||
Figure 1. Structrure of single-stranded DNA nanocircle composed of 63 nucleotides encoding a hammerhead ribozyme and 41 nucleotides of randomized sequences | Figure 1. Structrure of single-stranded DNA nanocircle composed of 63 nucleotides encoding a hammerhead ribozyme and 41 nucleotides of randomized sequences | ||
http://www.pnas.org/content/vol99/issue1/images/medium/pq0125890001.gif | http://www.pnas.org/content/vol99/issue1/images/medium/pq0125890001.gif | ||
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| + | Figure 2. Schematic of artificial ribozymes using error prone reverse transcripase PCR | ||
http://www.pnas.org/content/vol0/issue2001/images/data/012589099/DC1/5890Fig9.gif | http://www.pnas.org/content/vol0/issue2001/images/data/012589099/DC1/5890Fig9.gif | ||
Revision as of 07:56, 20 November 2007
Nanocircles are small circular single-stranded DNA that can be transcribed by phage and bacterial RNA polymerases. These plasmid-like structures were originally developed by Eric T. Kool's lab. The new technology uses a method called rolling circle transcription (RCT) to encode hammerhead, hairpin and hepatitis delta ribozymes.
Rolling Circle Animation Click on Rolling Circles & Artificial Telomeres
Experimental Design
Figure 1. Structrure of single-stranded DNA nanocircle composed of 63 nucleotides encoding a hammerhead ribozyme and 41 nucleotides of randomized sequences
http://www.pnas.org/content/vol99/issue1/images/medium/pq0125890001.gif
Figure 2. Schematic of artificial ribozymes using error prone reverse transcripase PCR
http://www.pnas.org/content/vol0/issue2001/images/data/012589099/DC1/5890Fig9.gif
