Difference between revisions of "Nanocircles"
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== Goals == | == Goals == | ||
| + | synthesize self-processing ribozymes | ||
| + | use ribozymes to regulate genes | ||
| + | change ribozymes/keep randomized domain (universality) | ||
| + | change gene to something useful | ||
| + | reinforce importance of secondary structure/cleavage site | ||
| + | |||
== Experimental Design == | == Experimental Design == | ||
Revision as of 08:19, 20 November 2007
Nanocircles are small circular single-stranded DNA that can be transcribed by phage and bacterial RNA polymerases. These plasmid-like structures were originally developed by Eric T. Kool's lab. The new technology uses a method called rolling circle transcription (RCT) to encode hammerhead, hairpin and hepatitis delta ribozymes.
Rolling Circle Animation Click on Rolling Circles & Artificial Telomeres
Goals
synthesize self-processing ribozymes use ribozymes to regulate genes change ribozymes/keep randomized domain (universality) change gene to something useful reinforce importance of secondary structure/cleavage site
Experimental Design
Figure 1. Structrure of single-stranded DNA nanocircle composed of 63 nucleotides encoding a hammerhead ribozyme and 41 nucleotides of randomized sequences
http://www.pnas.org/content/vol99/issue1/images/medium/pq0125890001.gif
Figure 2. Schematic of artificial ribozymes using error prone reverse transcripase PCR
http://www.pnas.org/content/vol0/issue2001/images/data/012589099/DC1/5890Fig9.gif
Results
Applications of Ribozymes in Synthetic Systems - Danielle Jordan
