Difference between revisions of "Nanocircles"

Revision as of 08:22, 20 November 2007

Nanocircles are small circular single-stranded DNA that can be transcribed by phage and bacterial RNA polymerases. These plasmid-like structures were originally developed by Eric T. Kool's lab. The new technology uses a method called rolling circle transcription (RCT) to encode hammerhead, hairpin and hepatitis delta ribozymes.

Rolling Circle Animation Click on Rolling Circles & Artificial Telomeres

Goals

synthesize self-processing ribozymes use ribozymes to regulate genes change ribozymes/keep randomized domain (universality) change gene to something useful reinforce importance of secondary structure/cleavage site

Experimental Design

Figure 1. Structrure of single-stranded DNA nanocircle composed of 63 nucleotides encoding a hammerhead ribozyme and 41 nucleotides of randomized sequences

http://www.pnas.org/content/vol99/issue1/images/medium/pq0125890001.gif

Figure 2. Schematic of artificial ribozymes using error prone reverse transcripase PCR

http://www.pnas.org/content/vol0/issue2001/images/data/012589099/DC1/5890Fig9.gif

Results

Applications of Ribozymes in Synthetic Systems - Danielle Jordan

http://www.pnas.org/content/vol99/issue1/images/medium/pq0125890004.gif