Difference between revisions of "Nanodrop DNA for Bio113"

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# Pipet 2 µL of your mini prep onto the small open stop on the metal circle.  
 
# Pipet 2 µL of your mini prep onto the small open stop on the metal circle.  
 
# Carefully lower the arm and click on the measure button.
 
# Carefully lower the arm and click on the measure button.
# Record these values for each of your samples:  
+
; Record these values for each of your samples:  
** absorbance value of your DNA at 260 nm
+
: absorbance value of your DNA at 260 nm
* concentration of your DNA in ng/µL
+
: concentration of your DNA in ng/µL
* 260/280 ratio value
+
: 260/280 ratio value

Latest revision as of 15:33, 3 January 2020

Nanodrop DNA

  1. The Nanodrop will be blanked using the elution buffer from your mini prep.
  2. Pipet 2 µL of your mini prep onto the small open stop on the metal circle.
  3. Carefully lower the arm and click on the measure button.
Record these values for each of your samples
absorbance value of your DNA at 260 nm
concentration of your DNA in ng/µL
260/280 ratio value