Oligos to Build

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Lac System:

Part Forward Reverse
luxS LuxS forward.jpg LuxS reverse.jpg
lacIQ1 Promoter Placiq1 forward.jpg ###
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Lsr System:

Part Forward Reverse
lsrR Lsr forward.jpg Lsr reverse.jpg
lsrK LsrK forward.jpg LsrK reverse.jpg
plsr Plsr forward.jpg Plsr reverse.jpg
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Sequences of Parts to Build

LuxS Wildtype from MG1655


ATGCCGTTGT TAGATAGCTT CACAGTCGAT CATACCCGGA TGGAAGCGCC TGCAGTTCGG GTGGCGAAAA CAATGAACAC CCCGCATGGC GACGCAATCA CCGTGTTCGA TCTGCGCTTC TGCGTGCCGA ACAAAGAAGT GATGCCAGAA AGAGGGATCC ATACCCTGGA GCACCTGTTT GCTGGTTTTA TGCGTAACCA TCTTAACGGT AATGGTGTAG AGATTATCGA TATCTCGCCA ATGGGCTGCC GCACCGGTTT TTATATGAGT CTGATTGGTA CGCCAGATGA GCAGCGTGTT GCTGATGCCT GGAAAGCGGC AATGGAAGAC GTGCTGAAAG TGCAGGATCA GAATCAGATC CCGGAACTGA ACGTCTACCA GTGTGGCACT TACCAGATGC ACTCGTTGCA GGAAGCGCAG GATATTGCGC GTAGCATTCT GGAACGTGAC GTACGCATCA ACAGCAACGA AGAACTGGCA CTGCCGAAAG AGAAGTTGCA GGAACTGCAC ATCTAG

Map Position: 2,812,240 <- 2,812,755 (nucleotides)

Sequence Length: 516 bp / 171 AAs

Mutated LuxS

Nucleotide position 54 changed from A to T in order to destroy a PstI site; this is a sense mutation

Also changed stop codon from TAG to TAA (recommended in Registry Help)

ATGCCGTTGT TAGATAGCTT CACAGTCGAT CATACCCGGA TGGAAGCGCC TGCTGTTCGG GTGGCGAAAA CAATGAACAC CCCGCATGGC GACGCAATCA CCGTGTTCGA TCTGCGCTTC TGCGTGCCGA ACAAAGAAGT GATGCCAGAA AGAGGGATCC ATACCCTGGA GCACCTGTTT GCTGGTTTTA TGCGTAACCA TCTTAACGGT AATGGTGTAG AGATTATCGA TATCTCGCCA ATGGGCTGCC GCACCGGTTT TTATATGAGT CTGATTGGTA CGCCAGATGA GCAGCGTGTT GCTGATGCCT GGAAAGCGGC AATGGAAGAC GTGCTGAAAG TGCAGGATCA GAATCAGATC CCGGAACTGA ACGTCTACCA GTGTGGCACT TACCAGATGC ACTCGTTGCA GGAAGCGCAG GATATTGCGC GTAGCATTCT GGAACGTGAC GTACGCATCA ACAGCAACGA AGAACTGGCA CTGCCGAAAG AGAAGTTGCA GGAACTGCAC ATCTAA

LsrR Gene

ATGACAATCA ACGATTCGGC AATTTCAGAA CAGGGAATGT GTGAAGAAGA ACAGGTCGCG CGGATCGCGT GGTTTTACTA TCACGACGGG CTGACCCAGA GCGAGATCAG CGATCGTCTC GGCCTGACAC GTTTGAAAGT GTCGCGATTG CTGGAGAAAG GGCATCAGTC CGGCATTATT CGCGTACAGA TTAATTCTCG CTTTGAAGGC TGTCTGGAAT ATGAAACTCA ATTACGTCGT CAGTTTTCGC TGCAACATGT CCGGGTGATC CCTGGGCTTG CGGATGCTGA TGTCGGTGGG CGACTGGGGA TAGGCGCGGC GCATATGTTG ATGAGTTTAC TTCAACCACA ACAGATGCTG GCGATTGGTT TTGGCGAGGC AACCATGAAT ACGCTGCAAC GCTTAAGTGG TTTTATTTCG TCACAGCAAA TTCGCCTGGT CACGCTCTCC GGTGGCGTCG GTTCTTATAT GACGGGAATC GGGCAGCTTA ACGCGGCGTG CAGTGTGAAT ATTATTCCGG CTCCGTTGCG GGCATCCTCC GCTGACATTG CCCGTACGCT AAAAAATGAA AATTGCGTCA AAGATGTTCT GTTAGCCGCG CAAGCAGCGG ATGTGGCGAT TGTCGGCATT GGTGCTGTGA GTCAACAGGA CGATGCGACA ATCATTCGCT CCGGTTATAT CAGCCAGGGC GAACAGTTAA TGATTGGCCG AAAAGGGGCG GTTGGCGACA TTTTAGGCTA CTTTTTTGAT GCAAAAGGTG ACGTTGTCAC GAATATCAAA ATACATAACG AACTGATTGG CTTACCTTTA AGCGCGCTGA AGACCATACC CGTCCGGGTT GGCGTGGCAG GGGGAGAAAA TAAAGCCGAA GCAATTGCCG CTGCAATGAA AGGCGGTTAT ATCAACGCAC TGGTTACCGA TCAGGACACA GCAGCGGCGA TTTTACGTAG TTAA

Map Position: 1,598,312 <- 1,599,265 (nucleotides)

Sequence Length: 954bp/317 AAs

LsrK Gene

atgGCTCGAC TCTTTACCCT TTCAGAATCA AAGTACTACC TGATGGCGCT GGATGCAGGC ACCGGAAGTA TTCGGGCTGT GATATTCGAC CTGGAAGGCA ATCAAATAGC AGTGGGACAG GCGGAGTGGC GGCATCTGGC AGTACCGGAC GTTCCTGGTT CTATGGAATT TGATCTCAAC AAAAACTGGC AACTGGCGTG TGAGTGTATG CGCCAGGCGC TGCACAACGC CGGCATAGCC CCGGAGTATA TCGCTGCCGT TTCGGCATGT TCGATGCGTG AAGGCATTGT TTTATATAAT AATGAAGGAG CCCCGATCTG GGCCTGCGCC AATGTGGATG CCAGAGCGGC ACGCGAAGTT AGCGAACTTA AAGAACTGCA CAACAATACC TTTGAAAACG AAGTTTATCG CGCGACCGGA CAAACACTGG CTTTAAGTGC CATCCCCAGA TTACTTTGGC TGGCGCACCA TCGTTCCGAT ATTTACCGTC AGGCATCAAC CATCACCATG ATCAGCGACT GGCTGGCCTA TATGCTCAGC GGCGAACTGG CGGTGGATCC CTCTAACGCT GGCACCACGG GACTTCTTGA TCTAACCACC CGTGACTGGA AACCTGCATT GCTGGATATG GCTGGCCTAC GTGCCGATAT TCTTTCTCCT GTCAAAGAAA CCGGCACATT GCTGGGCGTG GTAAGTTCAC AAGCGGCGGA ACTCTGCGGT CTGAAGGCGG GCACTCCGGT GGTCGTTGGA GGAGGCGACG TGCAGCTTGG TTGCCTTGGG TTAGGCGTTG TGCGTCCGGC ACAAACCGCG GTTCTTGGCG GCACATTCTG GCAGCAAGTT GTAAATTTAG CCGCGCCGGT GACAGACCCA GAAATGAACG TGCGCGTTAA TCCTCATGTT ATTCCTGGCA TGGTACAAGC TGAATCTATA AGCTTTTTTA CCGGACTCAC CATGCGCTGG TTCCGCGATG CTTTCTGTGC CGAAGAAAAA CTGATTGCCG AACGTTTAGG CATCGACACC TATACGCTGC TGGAAGAGAT GGCCAGTCGG GTGCCGCCTG GGTCGTGGGG CGTAATGCCG ATCTTCTCCG ACAGAATGCG CTTTAAAACC TGGTATCACG CTGCGCCTTC CTTTATTAAC TTGTCCATTG ACCCGGATAA ATGTAACAAA GCGACATTGT TCCGTGCGCT GGAAGAAAAT GCGGCGATTG TATCAGCGTG TAACTTGCAG CAAATTGCTG ATTTCTCGAA TATTCATCCT TCATCGTTAG TCTTTGCAGG CGGAGGTTCA AAAGGGAAAT TATGGAGTCA AATTCTCGCT GATGTCTCGG GATTACCCGT CAATATTCCG GTGGTCAAAG AAGCCACTGC ATTAGGATGT GCCATTGCAG CTGGCGTCGG TGCCGGAATT TTTTCATCAA TGGCAGAAAC CGGAGAACGC CTGGTTCGCT GGGAACGGAC GCACACACCA GACCCGGAAA AGCATGAACT TTATCAGGAT TCACGCGATA AGTGGCAGGC AGTTTATCAG GATCAGCTGG GGCTGGTTGA TCATGGACTG ACGACGTCGT TATGGAAAGC GCCTGGGTTA TAG

Map Position: 1,596,641 <- 1,598,233 (nucleotides)

Sequence Length: 1593 bp / 530 AAs

lsr promoter sequence
Plsr.jpg