Difference between revisions of "Origin of Replication"

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(Created page with "'''RNA I and RNA II gene and promoter sites in pSB1a2-BR''' File:PSB1A2_(pMB1)-_RNA_1_and_RNA_2_sites.docx‎ This is an link to the document containing the sequences fo...")
 
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This is an link to the document containing the sequences for RNA I and RNA II sites as well as their promoters. The document contains annotations as to the location of each element. *Note that the full length of RNA II is not fully known but is a close approximation.
 
This is an link to the document containing the sequences for RNA I and RNA II sites as well as their promoters. The document contains annotations as to the location of each element. *Note that the full length of RNA II is not fully known but is a close approximation.
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----
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'''Source Literature'''
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Replication and Control of Circular Bacterial Plasmids
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[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC98921/pdf/mr000434.pdf]
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 +
Plasmid ColE1 Incompatibility Determined by Interaction of RNA I
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with Primer Transcript
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[http://www.pnas.org/content/78/10/6096.full.pdf]
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Formation of an RNA Primer for Initiation of Replication of ColE1
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DNA by ribonuclease H
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[http://www.pnas.org/content/77/5/2450.full.pdf]
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Control of pMB1 Replication: Inhibition of Primer Formation
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by Rop Requires RNA1
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[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC557524/pdf/emboj00310-0150.pdf
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High Copy Number of the pUC Plasmid Results from a Rom/Rop--Suppressible Point Mutation in RNA II.
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[http://content.ebscohost.com/pdf9/pdf/1992/ESL/15Nov92/15903115.pdf?T=P&P=AN&K=15903115&S=R&D=eih&EbscoContent=dGJyMMTo50SeprU4yOvsOLCmr0uep65Ssa24SLSWxWXS&ContentCustomer=dGJyMK6nr0W2pq5T69fnhrnb5ofx6gAA]
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Control of ColE1 Replication: Low Affinity Specific Binding
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of Rop (Rom) to RNAI and RNAII
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[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC454354/pdf/emboj00139-0256.pdf]
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 +
Plasmid pT181 Replication is Regulated by Two Countertranscripts
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[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC397100/pdf/pnas00343-0012.pdf]
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The mutH Gene Regulates the Replication and Methylation
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of the pMB1 Origin
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[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC207916/pdf/jbacter00100-0187.pdf]
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 +
Attempt to Construct a Rop+ pUC19 from pBR322 Mutant Lacking
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the Tetracycline Resistance Gene
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[http://www.microbiology.ubc.ca/sites/default/files/roles/drupal_ungrad/JEMI/10/10-18.pdf]

Revision as of 14:48, 7 June 2013

RNA I and RNA II gene and promoter sites in pSB1a2-BR

File:PSB1A2 (pMB1)- RNA 1 and RNA 2 sites.docx

This is an link to the document containing the sequences for RNA I and RNA II sites as well as their promoters. The document contains annotations as to the location of each element. *Note that the full length of RNA II is not fully known but is a close approximation.


Source Literature

Replication and Control of Circular Bacterial Plasmids [1]

Plasmid ColE1 Incompatibility Determined by Interaction of RNA I with Primer Transcript [2]

Formation of an RNA Primer for Initiation of Replication of ColE1 DNA by ribonuclease H [3]

Control of pMB1 Replication: Inhibition of Primer Formation by Rop Requires RNA1 [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC557524/pdf/emboj00310-0150.pdf

High Copy Number of the pUC Plasmid Results from a Rom/Rop--Suppressible Point Mutation in RNA II. [4]

Control of ColE1 Replication: Low Affinity Specific Binding of Rop (Rom) to RNAI and RNAII [5]

Plasmid pT181 Replication is Regulated by Two Countertranscripts [6]

The mutH Gene Regulates the Replication and Methylation of the pMB1 Origin [7]

Attempt to Construct a Rop+ pUC19 from pBR322 Mutant Lacking the Tetracycline Resistance Gene [8]