Origin of Replication

RNA I and RNA II gene and promoter sites in pSB1a2-BR

File:PSB1A2 (pMB1)- RNA 1 and RNA 2 sites.docx

This is an link to the document containing the sequences for RNA I and RNA II sites as well as their promoters. The document contains annotations as to the location of each element. *Note that the full length of RNA II is not fully known but is a close approximation.

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Source Literature

Replication and Control of Circular Bacterial Plasmids [1]

Plasmid ColE1 Incompatibility Determined by Interaction of RNA I with Primer Transcript [2]

Formation of an RNA Primer for Initiation of Replication of ColE1 DNA by ribonuclease H [3]

Control of pMB1 Replication: Inhibition of Primer Formation by Rop Requires RNA1 [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC557524/pdf/emboj00310-0150.pdf

High Copy Number of the pUC Plasmid Results from a Rom/Rop--Suppressible Point Mutation in RNA II. [4]

Control of ColE1 Replication: Low Affinity Specific Binding of Rop (Rom) to RNAI and RNAII [5]

Plasmid pT181 Replication is Regulated by Two Countertranscripts [6]

The mutH Gene Regulates the Replication and Methylation of the pMB1 Origin [7]

Attempt to Construct a Rop+ pUC19 from pBR322 Mutant Lacking the Tetracycline Resistance Gene [8]