Origin of Replication
RNA I and RNA II gene and promoter sites in pSB1a2-BR
File:PSB1A2 (pMB1)- RNA 1 and RNA 2 sites.docx
This is an link to the document containing the sequences for RNA I and RNA II sites as well as their promoters. The document contains annotations as to the location of each element. *Note that the full length of RNA II is not fully known but is a close approximation.
RNA I/RNA II Literature
Replication and Control of Circular Bacterial Plasmids [1]
Plasmid ColE1 Incompatibility Determined by Interaction of RNA I with Primer Transcript [2]
Formation of an RNA Primer for Initiation of Replication of ColE1 DNA by ribonuclease H [3]
Control of pMB1 Replication: Inhibition of Primer Formation by Rop Requires RNA1 [4]
High Copy Number of the pUC Plasmid Results from a Rom/Rop--Suppressible Point Mutation in RNA II. [5]
Control of ColE1 Replication: Low Affinity Specific Binding of Rop (Rom) to RNAI and RNAII [6]
Plasmid pT181 Replication is Regulated by Two Countertranscripts [7]
The mutH Gene Regulates the Replication and Methylation of the pMB1 Origin [8]
Attempt to Construct a Rop+ pUC19 from pBR322 Mutant Lacking the Tetracycline Resistance Gene [9]
Quantitative PCR Literature
Improved Determination of Plasmid Copy Number Using Quantitative Real-Time PCR for Monitoring Fermentation Processes [10]
Fast SYBR Green Master Mix Protocol [11]
