Difference between revisions of "PMnt/Lac"
From GcatWiki
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pMnt/Lac is constitutively on but is repressed by the LacI protein. The promoter is also repressed by the Mnt protein. The addition of IPTG should repress the LacI repressor and glow in the absence of the Mnt protein. | pMnt/Lac is constitutively on but is repressed by the LacI protein. The promoter is also repressed by the Mnt protein. The addition of IPTG should repress the LacI repressor and glow in the absence of the Mnt protein. | ||
| + | |||
| + | Plan to Test the promoter | ||
Some promoter→LacI+t+t+pLac/Mnt→GFP does not glow | Some promoter→LacI+t+t+pLac/Mnt→GFP does not glow | ||
Revision as of 19:39, 30 June 2008
pMnt/Lac is constitutively on but is repressed by the LacI protein. The promoter is also repressed by the Mnt protein. The addition of IPTG should repress the LacI repressor and glow in the absence of the Mnt protein.
Plan to Test the promoter
Some promoter→LacI+t+t+pLac/Mnt→GFP does not glow
Some promoter→Mnt+t+t+pLac/Mnt→GFP does not glow
add IPTG
Some promoter→LacI+t+t+pLac/Mnt→GFP glows
Some promoter→LacI+Mnt+t+t+pLac/Mnt→GFP does not glow
| LacI | Mnt | IPTG + GFP | IPTG - GFP |
|---|---|---|---|
| 0 | 0 | 1 | 1 |
| 0 | 1 | 0 | 0 |
| 1 | 0 | 1 | 0 |
| 1 | 1 | 0 | 0 |
