Difference between revisions of "Post-transcriptional Regulation Technologies - Erin Zwack"
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Desai SK and Gallivan JP. Genetic Screens and Selections for Small Molecules Based on a Synthetic Riboswitch That Activates Protein Translation. ''J. Am. Chem. Soc.''(2004) 126:13247-54. | Desai SK and Gallivan JP. Genetic Screens and Selections for Small Molecules Based on a Synthetic Riboswitch That Activates Protein Translation. ''J. Am. Chem. Soc.''(2004) 126:13247-54. | ||
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Revision as of 05:53, 13 November 2007
Contents
Post-transcriptional Regulation Technologies
Overview:
Regulation of translation provides an excellent tool for research on metabolic and other pathways in organisms, and for the production of different sensors by controlling the translation of specific genes depending on cellular conditions. Further development of these types of technology could provide a “knock-down” equivalent to RNAi available in some eukaryotes. A gene of interest could be expressed normally at all times when the regulator is not active; thus, no ill effects will result before the pathway activates if the gene has another purpose as well. Other sythetic biologists could use these technologies to engineer fast-responding, RNA-based biological sensors for environmental chemicals, or novel pathways.
Development of Systems
In most cases, post-transcriptional regulatory mechanisms that were developed and worked in eukaryotes cannot be directly transferred to prokaryotes. Modifications are necessary because eukaryotic and prokaryotic transcription and translation do not follow the exact same path. In eukaryotes, mRNA must have introns spliced out before translation begins; thus, any mechanism that regulates translation has time to bind or manipulate the mRNA. In prokaryotes, translation begins as soon as the ribosomal binding site is transcribed and accessible to a ribosome.
The image above shows Eukaryotic transcription and translation.
The image above shows prokaryotic transcription and translation. Note that the processes are nearly simultaneous.
Eukaryotes:
Bayer TS and Smolke CD. Programmable ligand-controlled riboregulators of eukaryotic gene expression. Nat Biotechnol. (2005) 3:337-43.
Prokaryotes:
Isaacs FJ, et al. Engineered riboregulators enable post-transcriptional control of gene expression. Nat Biotechnol. (2004) 22:841-47.
Desai SK and Gallivan JP. Genetic Screens and Selections for Small Molecules Based on a Synthetic Riboswitch That Activates Protein Translation. J. Am. Chem. Soc.(2004) 126:13247-54.
