Difference between revisions of "Pouring an Agarose Gel"
From GcatWiki
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# Weigh out agarose in 125 ml flask (eg. 0.35 grams agarose for 1% gel). | # Weigh out agarose in 125 ml flask (eg. 0.35 grams agarose for 1% gel). | ||
| − | # Add 35 mL of TAE buffer | + | # Add 35 mL of 1X TAE buffer |
| − | # Microwave for | + | # Microwave for 50 seconds |
| − | # Swirl and microwave for | + | # Swirl and microwave for 5 seconds - repeat 3 times |
| − | # | + | # Place flask in 55 C water bath to cool |
| + | # Add 5 µL of 10 mg/ml Ethidium Bromide (CAUTION: WEAR GLOVES) | ||
# Swirl to mix and pour into gel tray with comb | # Swirl to mix and pour into gel tray with comb | ||
Revision as of 19:01, 15 February 2017
Pouring an Agarose Gel
- Go to http://www.bio.davidson.edu/courses/Molbio/Protocols/molwt.html to determine the percentage of agarose and calculate amount of agarose for 35 ml.
- Weigh out agarose in 125 ml flask (eg. 0.35 grams agarose for 1% gel).
- Add 35 mL of 1X TAE buffer
- Microwave for 50 seconds
- Swirl and microwave for 5 seconds - repeat 3 times
- Place flask in 55 C water bath to cool
- Add 5 µL of 10 mg/ml Ethidium Bromide (CAUTION: WEAR GLOVES)
- Swirl to mix and pour into gel tray with comb
