Difference between revisions of "Pouring an Agarose Gel"
From GcatWiki
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'''Pouring an Agarose Gel''' | '''Pouring an Agarose Gel''' | ||
| − | # | + | # Add 35 mL of 1X TAE buffer to a 125 ml flask |
| − | + | # Add agarose (0.35 grams agarose for 1% gel). | |
| − | # | ||
| − | |||
# Microwave for 50 seconds | # Microwave for 50 seconds | ||
# Swirl and microwave for 5 seconds - repeat 3 times | # Swirl and microwave for 5 seconds - repeat 3 times | ||
Revision as of 19:02, 15 February 2017
Pouring an Agarose Gel
- Add 35 mL of 1X TAE buffer to a 125 ml flask
- Add agarose (0.35 grams agarose for 1% gel).
- Microwave for 50 seconds
- Swirl and microwave for 5 seconds - repeat 3 times
- Place flask in 55 C water bath to cool
- Add 5 µL of 10 mg/ml Ethidium Bromide (CAUTION: WEAR GLOVES)
- Swirl to mix and pour into gel tray with comb
