Difference between revisions of "Ramping up Programmed Evolution"
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Macampbell (talk | contribs) (Created page with "# Riboswitch * Dual ligand * Turning on and off riboswitch # Increased variations in the module including the chaperones * Increase in variation within current categories. (...") |
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| − | + | 1) Riboswitch | |
* Dual ligand | * Dual ligand | ||
* Turning on and off riboswitch | * Turning on and off riboswitch | ||
| − | + | 2) Increased variations in the module including the chaperones | |
* Increase in variation within current categories. (Copy number, promoter strength) | * Increase in variation within current categories. (Copy number, promoter strength) | ||
* New category: mutation of individual base pairs | * New category: mutation of individual base pairs | ||
* Controlling interaction of riboswitch and aptamer | * Controlling interaction of riboswitch and aptamer | ||
| − | + | 3) Ecoli-yeast | |
* Temperature difference | * Temperature difference | ||
* Simple vs. complex pathway of translation | * Simple vs. complex pathway of translation | ||
| − | + | 4) The number of steps in the metabolic pathway | |
* The formation of byproducts | * The formation of byproducts | ||
* More than one compound of interest, possibly by decomposition. | * More than one compound of interest, possibly by decomposition. | ||
* The number of steps to reach differences in significant results. | * The number of steps to reach differences in significant results. | ||
| − | + | 5) Introducing mutations like in the promoter region, the ribosome binding site, elements found in nature like nickel, cadmium | |
| − | + | 6) Utilize other bacterial species i.e. B.subtilius | |
Revision as of 11:56, 21 May 2014
1) Riboswitch
- Dual ligand
- Turning on and off riboswitch
2) Increased variations in the module including the chaperones
- Increase in variation within current categories. (Copy number, promoter strength)
- New category: mutation of individual base pairs
- Controlling interaction of riboswitch and aptamer
3) Ecoli-yeast
- Temperature difference
- Simple vs. complex pathway of translation
4) The number of steps in the metabolic pathway
- The formation of byproducts
- More than one compound of interest, possibly by decomposition.
- The number of steps to reach differences in significant results.
5) Introducing mutations like in the promoter region, the ribosome binding site, elements found in nature like nickel, cadmium 6) Utilize other bacterial species i.e. B.subtilius
