Difference between revisions of "Rational design of memory in eukaryotic cells"

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[http://en.wikipedia.org/wiki/Zinc_fingers What are zinc fingers?]
 
[http://en.wikipedia.org/wiki/Zinc_fingers What are zinc fingers?]
  
Note, again, that in this paper, the creation of a bistable system relies on cooperative binding (in this case, cooperative binding of an activator). Pay attention to this when looking over the math modeling.
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Note, again, that in this paper, the creation of a bistable system relies on cooperative binding [http://en.wikipedia.org/wiki/Cooperativity] (in this case, cooperative binding of an activator). Pay attention to this when looking over the math modeling.
  
 
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[[Will DeLoache - Synthetic Biology Seminar | Back to Will's Main Page]]
 
[[Will DeLoache - Synthetic Biology Seminar | Back to Will's Main Page]]

Revision as of 02:08, 7 October 2007

Authors

Caroline Ajo-Franklin
David Drubin
Julian Eskin
Elain Gee
Dirk Landgraf
Ira Phillips
Pamela Silver

Methods

Flow Cytometry

Supplementary Material

Materials and Methods

Figure S1

Table S1

Table S2

Helpful Information

Keep in mind that the first half of the paper discusses the creation of an activator. This construct is intended to produce both red and yellow fluorescence in the presence of galactose. The second part of the paper discusses how this activator is then modified to serve as a sythetic memory device with three separate states:

Yeast that have never been in the presence of galactose will not fluoresce.
Yeast that are currently in the presence of galactose will fluoresce red (and yellow).
Yeast that have at one time been in the presence of galactose will fluoresce yellow.

What are zinc fingers?

Note, again, that in this paper, the creation of a bistable system relies on cooperative binding [1] (in this case, cooperative binding of an activator). Pay attention to this when looking over the math modeling.


Back to Will's Main Page