View source for Repeating Eckdahl's 20-clone selection

We need to replicate Dr. Eckdahl’s 20-clone experiment twice on both campuses and mathematically analyze the data. Continue adding arabinose to induce the chaperones.

We need to grow the surviving clones on individual plates: no antibiotic, amp, amp+chlor. This will enable us to determine whether both plasmids (CDM+amp, chaperone+chlor) are still present and being transcribed.

Depending on the survivorship of the clones in the previous step, set up plates to run head-to-head competition between two surviving clones, and determine which strain is most competent. This will confirm the results of the 20-clone experiment, in a more controlled environment than a plate with all 20 clones.

Protocol created on 05/21/14: [[Eckdahl-replication-protocol]]

[https://www.dropbox.com/sh/2jqkqfz755ng0vc/AAA26M9kEeYii0pFtFlCQ17na Photos from Davidson's first replication (four plates)] (Updated May 31, 2014)

Template for results: [[File:Replication Results Template.xlsx]]
Upload a file for each campus with the results displayed in the table. There is a tab in the file for each trial.