Difference between revisions of "Repeating Eckdahl's 20-clone selection"
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Protocol created on 05/21/14: [[Eckdahl-replication-protocol]] | Protocol created on 05/21/14: [[Eckdahl-replication-protocol]] | ||
| − | [https://www.dropbox.com/sh/2jqkqfz755ng0vc/AAA26M9kEeYii0pFtFlCQ17na Photos from Davidson's first replication (four plates)] | + | [https://www.dropbox.com/sh/2jqkqfz755ng0vc/AAA26M9kEeYii0pFtFlCQ17na Photos from Davidson's first replication (four plates)] (Updated May 30, 2014) |
Revision as of 17:22, 31 May 2014
We need to replicate Dr. Eckdahl’s 20-clone experiment twice on both campuses and mathematically analyze the data. Continue adding arabinose to induce the chaperones.
We need to grow the surviving clones on individual plates: no antibiotic, amp, amp+chlor. This will enable us to determine whether both plasmids (CDM+amp, chaperone+chlor) are still present and being transcribed.
Depending on the survivorship of the clones in the previous step, set up plates to run head-to-head competition between two surviving clones, and determine which strain is most competent. This will confirm the results of the 20-clone experiment, in a more controlled environment than a plate with all 20 clones.
Protocol created on 05/21/14: Eckdahl-replication-protocol
Photos from Davidson's first replication (four plates) (Updated May 30, 2014)
