Research

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There are 6 different ammeline riboswitches that have been created by Neil Dixon, John N. Duncan, Torsten Geerlings, Mark S. Dunstan, John E. G. McCarthy, David Leys, and Jason Micklefield. Their research is described in the article Reengineering orthogonally selective riboswitches. The article shows that the two best riboswitches seem to be M6 and M6C, however neither of these riboswitches appear to be truly "off". When there is no ammeline present there is still a production of GFP. This presents a problem because when using a fitness module like antibiotic resistant those cells that are not producing ammeline will still be able to live, not allowing for the evolution that we desire. There are two options that we can

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