Difference between revisions of "Riboswitch"
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| − | A | + | A ribozyme switch is a part of an mRNA that can directly bind to a small target molecule and whose binding affects the gene's ability. There are two aspects of a riboswitch, the aptamer and the expression platform. The aptamer portion binds to a target molecules and changes shape, affecting the expression platform, which is how gene expression is regulated. Some types of riboswitch mechanisms include: |
* Formation of transcription termination hairpins | * Formation of transcription termination hairpins | ||
* Blockage of translation by folding to isolate ribosome-binding sites | * Blockage of translation by folding to isolate ribosome-binding sites | ||
* Effect of folding on splicing of mRNA | * Effect of folding on splicing of mRNA | ||
* Self-cleavage ribozymes that cleave themselves in the presence of a target molecule | * Self-cleavage ribozymes that cleave themselves in the presence of a target molecule | ||
| + | This paper focuses on self-cleaving ribozymes with an aptamer sequence and a hammerhead ribozyme sequence | ||
See also [[Riboswitches]] for additional information | See also [[Riboswitches]] for additional information | ||
| + | |||
| + | Experimental Design | ||
Latest revision as of 10:54, 20 November 2007
A ribozyme switch is a part of an mRNA that can directly bind to a small target molecule and whose binding affects the gene's ability. There are two aspects of a riboswitch, the aptamer and the expression platform. The aptamer portion binds to a target molecules and changes shape, affecting the expression platform, which is how gene expression is regulated. Some types of riboswitch mechanisms include:
- Formation of transcription termination hairpins
- Blockage of translation by folding to isolate ribosome-binding sites
- Effect of folding on splicing of mRNA
- Self-cleavage ribozymes that cleave themselves in the presence of a target molecule
This paper focuses on self-cleaving ribozymes with an aptamer sequence and a hammerhead ribozyme sequence See also Riboswitches for additional information
Experimental Design
