Difference between revisions of "Ribozyme Switch"
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See also [[Riboswitches]] for additional information | See also [[Riboswitches]] for additional information | ||
== Experimental Design == | == Experimental Design == | ||
| + | http://www.pnas.org/content/vol104/issue36/images/medium/zpq0340773700001.gif | ||
== Link to Main Page == | == Link to Main Page == | ||
[[Applications of Ribozymes in Synthetic Systems - Danielle Jordan]] | [[Applications of Ribozymes in Synthetic Systems - Danielle Jordan]] | ||
Revision as of 11:11, 20 November 2007
A ribozyme switch is a part of an mRNA that can directly bind to a small target molecule and whose binding affects the gene's ability. There are two aspects of a riboswitch, the aptamer and the expression platform. The aptamer portion binds to a target molecules and changes shape, affecting the expression platform, which is how gene expression is regulated. Some types of riboswitch mechanisms include:
- Formation of transcription termination hairpins
- Blockage of translation by folding to isolate ribosome-binding sites
- Effect of folding on splicing of mRNA
- Self-cleavage ribozymes that cleave themselves in the presence of a target molecule
- This paper focuses on self-cleaving ribozymes with an aptamer sequence and a hammerhead ribozyme sequence
- Helix-slipping based switch
- Strand-displacement based switch
- This paper focuses on self-cleaving ribozymes with an aptamer sequence and a hammerhead ribozyme sequence
See also Riboswitches for additional information
Experimental Design
http://www.pnas.org/content/vol104/issue36/images/medium/zpq0340773700001.gif
Link to Main Page
Applications of Ribozymes in Synthetic Systems - Danielle Jordan
