Difference between revisions of "Ribozyme Switch"
| Line 1: | Line 1: | ||
| − | A ribozyme switch | + | A ribozyme switch is a part of an mRNA that can directly bind to a small target molecule and whose binding affects the gene's ability. There are two aspects of a riboswitch, the aptamer and the expression platform. The aptamer portion binds to a target molecules and changes shape, affecting the expression platform, which is how gene expression is regulated. Some types of riboswitch mechanisms include: |
* Formation of transcription termination hairpins | * Formation of transcription termination hairpins | ||
* Blockage of translation by folding to isolate ribosome-binding sites | * Blockage of translation by folding to isolate ribosome-binding sites | ||
Revision as of 08:01, 6 December 2007
A ribozyme switch is a part of an mRNA that can directly bind to a small target molecule and whose binding affects the gene's ability. There are two aspects of a riboswitch, the aptamer and the expression platform. The aptamer portion binds to a target molecules and changes shape, affecting the expression platform, which is how gene expression is regulated. Some types of riboswitch mechanisms include:
- Formation of transcription termination hairpins
- Blockage of translation by folding to isolate ribosome-binding sites
- Effect of folding on splicing of mRNA
- Self-cleavage ribozymes that cleave themselves in the presence of a target molecule
- This paper focuses on self-cleaving ribozymes with an aptamer sequence and a hammerhead ribozyme sequence
- Strand-Displacement based switch
- Helix-Slipping based switch
- This paper focuses on self-cleaving ribozymes with an aptamer sequence and a hammerhead ribozyme sequence
See also Riboswitches for additional information
Contents
Experimental Design
http://www.pnas.org/content/vol104/issue36/images/large/zpq0340773700001.jpeg
Strand-Displacement
Helix-Slipping
Results
Modularity and Specificity of Strand-Displacement-based Ribozyme Switches
http://www.pnas.org/content/vol104/issue36/images/medium/zpq0340773700005.gif
Examples of Modularity of Various Ribozyme switches in Cellular Engineering Applications
http://www.pnas.org/content/vol104/issue36/images/medium/zpq0340773700006.gif
Link to Main Page
Applications of Ribozymes in Synthetic Systems - Danielle Jordan
