Difference between revisions of "Ribozyme Switch"
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− | == [[Strand-Displacement]] == | + | === [[Strand-Displacement]] === |
− | == [[Helix-Slipping]] == | + | === [[Helix-Slipping]] === |
== Results == | == Results == |
Revision as of 08:03, 6 December 2007
A ribozyme switch is a part of an mRNA that can directly bind to a small target molecule and whose binding affects the gene's ability. There are two aspects of a riboswitch, the aptamer and the expression platform. The aptamer portion binds to a target molecules and changes shape, affecting the expression platform, which is how gene expression is regulated. Some types of riboswitch mechanisms include:
- Formation of transcription termination hairpins
- Blockage of translation by folding to isolate ribosome-binding sites
- Effect of folding on splicing of mRNA
- Self-cleavage ribozymes that cleave themselves in the presence of a target molecule
- This paper focuses on self-cleaving ribozymes with an aptamer sequence and a hammerhead ribozyme sequence
- Strand-Displacement based switch
- Helix-Slipping based switch
- This paper focuses on self-cleaving ribozymes with an aptamer sequence and a hammerhead ribozyme sequence
See also Riboswitches for additional information
Contents
Experimental Design
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Strand-Displacement
Helix-Slipping
Results
Modularity and Specificity of Strand-Displacement-based Ribozyme Switches
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Examples of Modularity of Various Ribozyme switches in Cellular Engineering Applications
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Link to Main Page
Applications of Ribozymes in Synthetic Systems - Danielle Jordan