User contributions
From GcatWiki
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- 15:00, 21 June 2018 (diff | hist) . . (+376) . . DNA Sequencing at Eurofins (current)
- 18:25, 18 June 2018 (diff | hist) . . (+22) . . Zymo Research Clean and Concentrate for Plasmid DNA (current)
- 18:23, 18 June 2018 (diff | hist) . . (+2) . . Zymo Research Clean and Concentrate for Plasmid DNA
- 18:23, 18 June 2018 (diff | hist) . . (+104) . . Zymo Research Clean and Concentrate for Plasmid DNA
- 20:10, 14 June 2018 (diff | hist) . . (+14) . . Zymo Research Clean and Concentrate for Plasmid DNA
- 20:09, 14 June 2018 (diff | hist) . . (+1) . . Zymo Research Clean and Concentrate for Plasmid DNA
- 20:09, 14 June 2018 (diff | hist) . . (+8) . . Zymo Research Clean and Concentrate for Plasmid DNA
- 20:08, 14 June 2018 (diff | hist) . . (+710) . . N Zymo Research Clean and Concentrate for Plasmid DNA (Created page with "Clean and Concentrate Protocol When using plasmid DNA that has been through Miniprep Protocol. 1. Combine 2 volumes of DNA Binding Buffer to each volume of plasmid. (Ex. 200...")
- 20:08, 14 June 2018 (diff | hist) . . (+57) . . MWSU protocols (current)
- 15:27, 16 February 2017 (diff | hist) . . (+50) . . MWSU protocols
- 15:24, 16 February 2017 (diff | hist) . . (+449) . . N DNA Sequencing at Eurofins (Created page with "# Prepare template and primer solutions at the concentrations listed at the bottom of this page. # Grab the appropriate number of bar-code tubes to be sent off for sequencing....")
- 21:14, 15 February 2017 (diff | hist) . . (+32) . . MWSU protocols
- 19:57, 15 February 2017 (diff | hist) . . (+2,086) . . N How to make a new Registry page (Created page with "'''Making a Registry Page''' # Go to Registry of Biological Parts then login as the correct username # Hit the tools tab and click add a part # Click add a Basic Part # Allow...") (current)
- 19:05, 15 February 2017 (diff | hist) . . (+37) . . MWSU protocols
- 19:03, 15 February 2017 (diff | hist) . . (+1) . . Pouring an Agarose Gel (current)
- 19:03, 15 February 2017 (diff | hist) . . (-1) . . Pouring an Agarose Gel
- 19:02, 15 February 2017 (diff | hist) . . (-161) . . Pouring an Agarose Gel
- 19:01, 15 February 2017 (diff | hist) . . (+39) . . Pouring an Agarose Gel
- 16:15, 9 February 2017 (diff | hist) . . (+3) . . Golden Gate Assembly Protocol (current)
- 16:12, 9 February 2017 (diff | hist) . . (+45) . . Golden Gate Assembly Protocol
- 15:39, 9 February 2017 (diff | hist) . . (0) . . Golden Gate Assembly Protocol
- 21:39, 2 February 2017 (diff | hist) . . (+208) . . Ligation and Transformation (current)
- 21:33, 2 February 2017 (diff | hist) . . (+288) . . Ligation and Transformation
- 19:44, 1 March 2016 (diff | hist) . . (0) . . Golden Gate Assembly Protocol
- 19:22, 1 March 2016 (diff | hist) . . (+239) . . Golden Gate Assembly Protocol
- 19:08, 1 March 2016 (diff | hist) . . (+11) . . Golden Gate Assembly Protocol
- 19:06, 1 March 2016 (diff | hist) . . (+14) . . Golden Gate Assembly Protocol
- 14:53, 4 June 2015 (diff | hist) . . (-11) . . Ethanol Precipitation of Vector DNA (current)
- 13:39, 2 June 2015 (diff | hist) . . (+893) . . N Gradient/Standard PCR (Created page with " Gradient/ Standard PCR 1. Create standard PCR mix. 10ul 2X GoTaq Green PCR mix 1ul forward primer (10mMl) 1ul reverse primer (10mM) 7ul dH2O 19ul Total Add 1ul of ...") (current)
- 13:33, 2 June 2015 (diff | hist) . . (+9) . . MWSU protocols
- 13:31, 2 June 2015 (diff | hist) . . (+41) . . Standard PCR (current)
- 13:17, 2 June 2015 (diff | hist) . . (+371) . . Standard PCR
- 19:32, 1 June 2015 (diff | hist) . . (+954) . . N Golden Gate Assembly Single Molecule Protocol (Created page with "Golden Gate Assembly Single Molecule Protocol GGA mixture contains: :1 µL (50 ng) Plasmid :1 µL 10X Promega Ligase Buffer :7 µL dH<sub>2</sub>O :0.5 µL Restrictio...") (current)
- 19:28, 1 June 2015 (diff | hist) . . (+51) . . MWSU protocols
- 19:27, 1 June 2015 (diff | hist) . . (+977) . . N Golden Gate Assembly Protocol (Created page with "Golden Gate Assembly Protocol GGA mixture contains: :1 µL (50 ng) Plasmid :1 µL promoter or other insert :1 µL 10X Promega Ligase Buffer :6 µL dH<sub>2</sub>O :0...")
- 19:27, 1 June 2015 (diff | hist) . . (-10) . . MWSU protocols
- 19:26, 1 June 2015 (diff | hist) . . (+6) . . Golden Gate Assembly Protocol for BsmB1
- 19:25, 1 June 2015 (diff | hist) . . (-45) . . Golden Gate Assembly Protocol for BsmB1
- 14:22, 20 May 2015 (diff | hist) . . (-1) . . Measuring Fluorescence in Bacteria (current)
- 14:21, 20 May 2015 (diff | hist) . . (-451) . . Measuring Fluorescence in Bacteria
- 14:13, 19 May 2015 (diff | hist) . . (-355) . . Fragment Purification (current)
- 20:42, 6 February 2015 (diff | hist) . . (-6) . . References 2015 (current)
- 20:40, 6 February 2015 (diff | hist) . . (+65) . . References 2015
- 21:20, 3 February 2015 (diff | hist) . . (-31) . . References 2015 (Blanked the page)
- 21:19, 3 February 2015 (diff | hist) . . (-77) . . References 2015
- 21:18, 3 February 2015 (diff | hist) . . (-6) . . References 2015
- 21:17, 3 February 2015 (diff | hist) . . (+6) . . References 2015
- 21:16, 3 February 2015 (diff | hist) . . (-6) . . References 2015
- 21:15, 3 February 2015 (diff | hist) . . (+39) . . References 2015
- 21:13, 3 February 2015 (diff | hist) . . (+15) . . References 2015
- 21:03, 3 February 2015 (diff | hist) . . (-15) . . File (Blanked the page) (current)
- 21:02, 3 February 2015 (diff | hist) . . (+15) . . N File (Created page with "Media:Alper")
- 20:58, 3 February 2015 (diff | hist) . . (+60) . . References 2015
- 20:56, 3 February 2015 (diff | hist) . . (-220) . . References 2015 (Blanked the page)
- 20:54, 3 February 2015 (diff | hist) . . (+19) . . References 2015
- 16:49, 3 February 2015 (diff | hist) . . (-57) . . References 2015
- 16:41, 3 February 2015 (diff | hist) . . (-7) . . References 2015
- 16:40, 3 February 2015 (diff | hist) . . (+98) . . References 2015
- 16:37, 3 February 2015 (diff | hist) . . (+92) . . References 2015
- 16:31, 3 February 2015 (diff | hist) . . (+17) . . References 2015
- 16:29, 3 February 2015 (diff | hist) . . (+58) . . N References 2015 (Created page with " Tuning Genetic Control through Promoter Engineering File:")
- 15:42, 3 February 2015 (diff | hist) . . (+1) . . Summer 2014 SynBio Project (Davidson and MWSU) (current)
- 15:42, 3 February 2015 (diff | hist) . . (+20) . . Summer 2014 SynBio Project (Davidson and MWSU)
- 20:58, 7 July 2014 (diff | hist) . . (-10) . . Electroporation Transformation
- 18:56, 7 July 2014 (diff | hist) . . (-39) . . Electroporation Transformation
- 15:42, 10 March 2014 (diff | hist) . . (+65) . . Electroporation Transformation
- 14:39, 10 March 2014 (diff | hist) . . (+6) . . Electroporation Transformation
- 14:38, 10 March 2014 (diff | hist) . . (+16) . . Electroporation Transformation
- 14:37, 10 March 2014 (diff | hist) . . (+1) . . Electroporation Transformation
- 14:36, 10 March 2014 (diff | hist) . . (+1,682) . . N Electroporation Transformation (Created page with 'This procedure uses electroporation to achieve transformation of bacteria with plasmid DNA. '''Cell Preparation''' 1. This procedure yields enough bacteria for two transformati…')
- 14:15, 10 March 2014 (diff | hist) . . (+36) . . MWSU protocols
- 15:02, 1 February 2014 (diff | hist) . . (+69) . . Fragment Purification
- 14:42, 1 February 2014 (diff | hist) . . (+95) . . Fragment Purification
- 14:36, 1 February 2014 (diff | hist) . . (+43) . . Fragment Purification
- 14:34, 1 February 2014 (diff | hist) . . (+169) . . Fragment Purification
- 20:25, 16 January 2014 (diff | hist) . . (+1,407) . . N Zymo Research Plasmid Minipreps (Created page with "Zymo Research Zippy Plasmid Minipreps Zymo Research: http://www.zymoresearch.com/ Cat. D4036, D4037 The kit suggests starting with 600 ul of overnight bacterial culture. W...") (current)
- 20:10, 16 January 2014 (diff | hist) . . (+37) . . MWSU protocols
- 19:42, 31 May 2013 (diff | hist) . . (-1) . . J-GGA Scaffold Sequences
- 18:42, 31 May 2013 (diff | hist) . . (+874) . . N J-GGA Scaffold Sequences (Created page with 'J-GGA Scaffold Oligos Prefix: Gaattcgcggccgcttctagag Junction A: Atagtctgttcatggtgc Spacer: acgt Junction B: Tattgatgtagttaaggc Spacer: cgga Junction C: Tttcta…')
- 18:41, 31 May 2013 (diff | hist) . . (+9) . . Biology
- 18:33, 31 May 2013 (diff | hist) . . (+22) . . Biology (Junction Golden Gate Assembly Sequences)
- 19:49, 20 May 2013 (diff | hist) . . (+7) . . IPCR (current)
- 19:48, 20 May 2013 (diff | hist) . . (+14) . . Golden Gate Assembly Protocol for BsmB1
- 19:46, 20 May 2013 (diff | hist) . . (+124) . . GCAT Library of Quality Parts (current)
- 19:44, 20 May 2013 (diff | hist) . . (+114) . . MWSU Freezer Parts (current)
- 19:41, 20 May 2013 (diff | hist) . . (+2,913) . . N MWSU Freezer Parts (MWSU Freezer Parts)
- 19:40, 20 May 2013 (diff | hist) . . (+6,581) . . N GCAT Library of Quality Parts (GCAT Library of Quality Parts)
- 19:40, 20 May 2013 (diff | hist) . . (+35) . . MWSU protocols
- 19:37, 20 May 2013 (diff | hist) . . (+6,581) . . N DNA glycerol stock (GCAT Library of Quality Parts) (current)
- 19:35, 20 May 2013 (diff | hist) . . (0) . . MWSU protocols
- 19:34, 20 May 2013 (diff | hist) . . (+46) . . MWSU protocols
- 19:32, 20 May 2013 (diff | hist) . . (+744) . . N IPCR (Inverted PCR/Biobrick Cloning)
- 19:31, 20 May 2013 (diff | hist) . . (+10) . . MWSU protocols
- 18:26, 27 June 2012 (diff | hist) . . (+129) . . N Philosophy and Ethics of our Project (Created page with 'This page is for the Philosophical and Ethical considerations surrounding our project to optimize a metabolic pathway in E. coli.')
- 18:25, 27 June 2012 (diff | hist) . . (+47) . . Summer 2012 SynBio Project (Davidson and MWSU)
- 14:13, 27 June 2012 (diff | hist) . . (-1) . . m Annealing Oligos for Cloning (Change in boiling time for annealing)
- 18:50, 25 May 2012 (diff | hist) . . (0) . . Ligation and Transformation
- 19:03, 23 May 2012 (diff | hist) . . (+39) . . Summer 2012 SynBio Project (Davidson and MWSU) (→Questions to Consider About Network Pathways)
- 18:34, 23 May 2012 (diff | hist) . . (+239) . . Summer 2012 SynBio Project (Davidson and MWSU)
- 18:23, 23 May 2012 (diff | hist) . . (+69) . . Summer 2012 SynBio Project (Davidson and MWSU) (→Papers)
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