Difference between revisions of "Status Of Building Parts"
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|P0440|| Produces tetR | |P0440|| Produces tetR | ||
|- | |- | ||
+ | |I13263|| Lux Receiver | ||
+ | |- | ||
+ | |I0407|| Produces lasI and outputs ECFP | ||
+ | |- | ||
+ | |I0466||RhlR protein generator | ||
+ | |- | ||
+ | |K09100|| AHL receiver and outputs GFP | ||
+ | |- | ||
+ | |J04430|| GFP generator switched on by IPTG | ||
+ | |- | ||
+ | |E0240||GFP generator | ||
+ | |- | ||
+ | |F2620|| Accepts 30C6HSL as an input and outputs PoPS | ||
+ | |- | ||
+ | | | ||
|} | |} | ||
[http://parts.mit.edu/igem07/index.php/USTC '''Look at USTC 2007 for logic gates'''] | [http://parts.mit.edu/igem07/index.php/USTC '''Look at USTC 2007 for logic gates'''] |
Revision as of 19:42, 17 June 2008
MWSU + Davidson Status of Building Sender and Receiver
plux-lac hybrid promoter:
- I751502
- In the process of building another plux-lac hybrid promoter that is repressed by HSL and lacI
Responsible: Robert Cool, Erin Feeney and Pallavi Penumetcha
Parts we have Mini-Preps and Digestions for
Part | Description | Size of Insert (w/ BioBrick ends) | Verification using Gel Electrophoresis |
---|---|---|---|
BBa_F2620 | Accepts 3OC6HSL as an input and outputs PoPS | 1102 bp | Appears to be reversed with J04430 |
BBa_J04430 | GFP generator switched on by IPTG | 1124 bp | Appears to be reversed with F2620 |
BBa_P0440 | Produces tetR | 881 bp | Verified |
BBa_E0240 | GFP generator | 917 bp | Verified |
BBa_I132623 | Lux receiver that produces luxR and outputs EYFP | 2085 bp | Much smaller on gel |
BBa_I0426 | Produces lasR and outputs ECFP followed by a lasR-PAI regulated promoter that outputs EYFP | 2839 bp | Verified |
BBa_C0061 | Produces luxI (w/ LVA tag) | 659 bp | Verified |
BBa_C0261 | Produces luxI (w/ RBS) | 702 bp | No successful Mini-Preps |
BBa_R0062 | luxR and HSL regulated promoter | 96 bp | Verified |
BBa_I0466 | RhlR protein generator | 983 bp | Verified |
BBa_I0407 | Produces lasI and outputs ECFP | 1674 bp | Verified |
BBa_R0079 | LasR and PAI regulated promoter | 198 bp | Verified |
BBa_R0063 | LuxR and HSL regulated promoter | 192 bp | No successful Mini-Preps |
BBa_K09100 | Receiver for AHL and outputs GFP | 1987 bp | Verified |
BBa_J04450 | RFP coding device switched on by IPTG | 2110 bp | Much smaller on gel |
-We will be sending all of these parts to be sequenced
Gels used to verify parts
Gel A
These are the parts run on a 0.5% gel.
Top portion of Gel A
Lane | Part |
---|---|
1 | MW |
2 | F0260 |
3 | J04430 |
4 | P0440 |
5 | E0240 |
6 | I13263 |
7 | J0440 |
Bottom portion of Gel A
Lane | Part |
---|---|
1 | MW |
2 | C0061 |
3 | I0466 |
4 | I0407 |
5 | I0426 |
6 | K09100 |
GelB
These parts were run on a 2.5% gel.
Lane | Part |
---|---|
1 | MW |
2 | R0062 |
3 | R0079 |
9 | pTet-lac PCR product |
10 | MW |
Parts we have Glycerol Stocks for
Part | Description |
---|---|
R0062 | LuxR and HSL regulated promoter |
R0063 | LuxR and HSL regulate promoter |
P0440 | Produces tetR |
I13263 | Lux Receiver |
I0407 | Produces lasI and outputs ECFP |
I0466 | RhlR protein generator |
K09100 | AHL receiver and outputs GFP |
J04430 | GFP generator switched on by IPTG |
E0240 | GFP generator |
F2620 | Accepts 30C6HSL as an input and outputs PoPS |
Look at USTC 2007 for logic gates
T9002 - no colonies
F2622 + E0240 (ligated/colonies coming up)
+
S03632 (ligations needed)
But BBa_S03632 is not available, so we will need to build our own version using the 3 parts below:
Parts: R0011 + C0261 + B0015
+ +
R0011 + F1610 (minis, frag needed/colonies coming up, minis thurs)
+
F2620 + E0240 (minis growing/ frag available)
+