Status Of Building Parts

MWSU + Davidson Status of Building Sender and Receiver

plux-lac hybrid promoter:

• I751502
• In the process of building another plux-lac hybrid promoter that is repressed by HSL and lacI

Responsible: Robert Cool, Erin Feeney and Pallavi Penumetcha

Parts we have Mini-Preps and Digestions for

Part	Description	Size of Insert (w/ BioBrick ends)	Verification using PCR
BBa_F2620	Accepts 3OC6HSL as an input and outputs PoPS	1102 bp	Appears to be reversed with J04430
BBa_J04430	GFP generator switched on by IPTG	1124 bp	Appears to be reversed with F2620
BBa_P0440	Produces tetR	881 bp	Verified
BBa_E0240	GFP generator	917 bp	Verified
BBa_I132623	Lux receiver that produces luxR and outputs EYFP	2085 bp	Much smaller on gel
BBa_I0426		2839 bp	Verified
BBa_C0061	Produces luxI (w/ LVA tag)	659 bp	Verified
BBa_C0261	Produces luxI (w/ RBS)	702 bp	No successful Mini-Preps
BBa_R0062	luxR and HSL regulated promoter	96 bp	Verified
BBa_I0466	RhlR protein generator	983 bp	Verified
BBa_I0407	Produces lasI and outputs ECFP	1674 bp	Verified
BBa_R0079	LasR and PAI regulated promoter	198 bp	Verified
BBa_R0063	LuxR and HSL regulated promoter	192 bp	Have not yet digested
BBa_K09100	Receiver for AHL and outputs GFP	1987 bp	Verified
BBa_J04450	RFP coding device switched on by IPTG	2110 bp	Much smaller on gel

Look at USTC 2007 for logic gates

T9002 - no colonies

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F2622 + E0240 (ligated/colonies coming up)
+

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S03632 (ligations needed)

But BBa_S03632 is not available, so we will need to build our own version using the 3 parts below:
Parts: R0011 + C0261 + B0015
+ +

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R0011 + F1610 (minis, frag needed/colonies coming up, minis thurs)
+

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F2620 + E0240 (minis growing/ frag available)
+

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P0440