Difference between revisions of "Strand-Displacement"
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| − | The strand-displacement mechanism uses competitive binding of two identical nucleic acid sequences, the | + | The strand-displacement mechanism uses competitive binding of two identical nucleic acid sequences, the competing strand and the switching strand. It is based on rational design and results in the disruption or restoration of the hammerhead ribozyme as a result of restoration in the aptamer domain. |
== Competing strand == | == Competing strand == | ||
The competing strand is the nucleic acid sequence that is bound to the the general transmission region in the restored switch conformation in the presence of a ligand | The competing strand is the nucleic acid sequence that is bound to the the general transmission region in the restored switch conformation in the presence of a ligand | ||
| − | == | + | == Switching strand == |
The switching strand is the nucleic acid sequence that is bound to the general transmission region in the disrupted switch conformation in the absense of a ligand | The switching strand is the nucleic acid sequence that is bound to the general transmission region in the disrupted switch conformation in the absense of a ligand | ||
http://www.pnas.org/content/vol104/issue36/images/large/zpq0340773700002.jpeg | http://www.pnas.org/content/vol104/issue36/images/large/zpq0340773700002.jpeg | ||
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| + | http://www.pnas.org/content/vol104/issue36/images/large/zpq0340773700004.jpeg | ||
== Links == | == Links == | ||
Revision as of 11:47, 20 November 2007
The strand-displacement mechanism uses competitive binding of two identical nucleic acid sequences, the competing strand and the switching strand. It is based on rational design and results in the disruption or restoration of the hammerhead ribozyme as a result of restoration in the aptamer domain.
Competing strand
The competing strand is the nucleic acid sequence that is bound to the the general transmission region in the restored switch conformation in the presence of a ligand
Switching strand
The switching strand is the nucleic acid sequence that is bound to the general transmission region in the disrupted switch conformation in the absense of a ligand
http://www.pnas.org/content/vol104/issue36/images/large/zpq0340773700002.jpeg
http://www.pnas.org/content/vol104/issue36/images/large/zpq0340773700004.jpeg
Links
Applications of Ribozymes in Synthetic Systems - Danielle Jordan
