Difference between revisions of "Summer 2013 SynBio Project (Davidson and MWSU)"
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* Determine the junction sequences and associated primers<br> | * Determine the junction sequences and associated primers<br> | ||
(use existing rules)<br> | (use existing rules)<br> | ||
| − | build scaffold <br> | + | build scaffold for insert segments<br> |
| + | include orI (collaborate with copy number research)<br> | ||
| + | include drug resistance gene<br> | ||
test JGG design<br> | test JGG design<br> | ||
* Stress module needs more work (see eCDM8 outputs)<br> | * Stress module needs more work (see eCDM8 outputs)<br> | ||
| Line 20: | Line 22: | ||
* Copy number of plasmids <br> | * Copy number of plasmids <br> | ||
Can we alter Ori to produce a range of plasmid densities in ''E. coli''? <br> | Can we alter Ori to produce a range of plasmid densities in ''E. coli''? <br> | ||
| + | * Does theophylline diffuse across the membrane and thus the system fails<br> | ||
| − | |||
| + | <u>Second Order Priority = one person</u> | ||
| + | * produce different CDM alleles | ||
| + | * swap out different components (promoters, RBS, alleles) | ||
| + | * test out programmed evolution | ||
<center>'''Mathematics'''</center> | <center>'''Mathematics'''</center> | ||
Revision as of 14:23, 30 May 2013
Google Hangout 11 am Wednesday (10 Central)
Topics to Investigate
High Priority = more people
- eCDM8 working to make theophylline with biosensor and fitness modules (Tet^R) to report out.
Which plasmid carries which device?
- Determine the junction sequences and associated primers
(use existing rules)
build scaffold for insert segments
include orI (collaborate with copy number research)
include drug resistance gene
test JGG design
- Stress module needs more work (see eCDM8 outputs)
- test fitness module with adhE
compare with tetA
fine tune tetA resistance
combine with tetA
- Copy number of plasmids
Can we alter Ori to produce a range of plasmid densities in E. coli?
- Does theophylline diffuse across the membrane and thus the system fails
Second Order Priority = one person
- produce different CDM alleles
- swap out different components (promoters, RBS, alleles)
- test out programmed evolution
