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| Google Hangout 11 am Wednesday (10 Central) | | Google Hangout 11 am Wednesday (10 Central) |
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− | <center>'''Biology'''</center> | + | <center>==[[Biology]]==</center> |
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− | '''Topics to Investigate'''
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− | <u>High Priority = more people</u>
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− | * eCDM8 working to make theophylline with biosensor and fitness modules (Tet^R) to report out. <br>
| + | <center>==[[Mathematics]]==</center> |
− | Which plasmid carries which device?<br>
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− | * Determine the junction sequences and associated primers<br>
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− | (use existing rules)<br>
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− | build scaffold for insert segments<br>
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− | include orI (collaborate with copy number research)<br>
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− | include drug resistance gene<br>
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− | test JGG design<br>
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− | * Stress module needs more work (see eCDM8 outputs)<br>
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− | * test fitness module with adhE
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− | compare with tetA<br>
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− | fine tune tetA resistance<br>
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− | combine with tetA<br>
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− | * Copy number of plasmids <br>
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− | Can we alter Ori to produce a range of plasmid densities in ''E. coli''? <br>
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− | * Does theophylline diffuse across the membrane and thus the system fails<br>
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− | <u>Second Order Priority = one person</u>
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− | * produce different CDM alleles
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− | * swap out different components (promoters, RBS, alleles)
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− | * test out programmed evolution
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− | <center>'''Mathematics'''</center>
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