Summer 2013 SynBio Project (Davidson and MWSU)

Google Hangout 11 am Wednesday (10 Central)

Biology

Topics to Investigate

High Priority = more people

• eCDM8 working to make theophylline with biosensor and fitness modules (Tet^R) to report out.
  

Which plasmid carries which device?

• Determine the junction sequences and associated primers
  

(use existing rules)
build scaffold for insert segments
include orI (collaborate with copy number research)
include drug resistance gene
test JGG design

• Stress module needs more work (see eCDM8 outputs)
  
• test fitness module with adhE

compare with tetA
fine tune tetA resistance
combine with tetA

• Copy number of plasmids
  

Can we alter Ori to produce a range of plasmid densities in E. coli?

• Does theophylline diffuse across the membrane and thus the system fails
  

Second Order Priority = one person

• produce different CDM alleles
• swap out different components (promoters, RBS, alleles)
• test out programmed evolution

Mathematics