Suppressor Logic Toolkit and Quasi-Hash Function

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Suppressor Logic Toolkit and Quasi-Hash Function

The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function

Basic Toolkit Design

The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors,

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Toolkit with Specific Inputs and Outputs

PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.

See references at bottom of page.

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Experimental Design

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Parts Needed

Parts from Registry

Promoters

pR R0051

pLacIQ1 K091112

pTetR R0040

pLuxR R0062

pMnt R0073

Genes

RBS+lamba cI+TT K082022

pBAD+RBS+LacI+TT S03980

pBAD+RBS+tetR+TT K091145

pBAD+RBS+luxR+TT K091179

RBS(B0030)+GFP E5500

RBS(B0030)+RFP S04120

Mnt C0072

LuxO-P K131022

Parts needed to be built from scratch

Pprp

prpB 2-methylisocitrate lyase in E.coli MG1655 [1]

pLuxT

frame shift suppressor 1

frame shift suppressor 2

References

Powerpoint File:Suppressorlogic3.ppt

Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [2]

luxO luxT [3]

PprpB propionate [4]

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