Suppressor Logic Toolkit and Quasi-Hash Function

Suppressor Logic Toolkit and Quasi-Hash Function

The following slides describe the basic toolkit design and a specific experiment that would result in a quasi-hash function

Basic Toolkit Design

The AND gate was designed by Anderson using termination suppressors, while the new design would use frame shift suppressors.

Toolkit with Specific Inputs and Outputs

PprpB/propionate and luxO/pluxT are new communication players that would need to be looked into further to verify their usefulness.

See references at bottom of page.

Experimental Design

Parts Needed

Parts from Registry

Promoters

pR R00512009 Kit Plate 1 6K pSB1A2

pLacIQ1 K091112MWSU

pTetR R00402009 Kit Plate 1 6I pSB1A2

pLuxR R00622009 Kit Plate 1 6O pSB1A2

pMnt R00732009 Kit Plate 1 16B pSB2K3

Genes

RBS+lamba cI+TT P04512009 Kit Plate 2 1N pSB1AK3

pBAD+RBS+LacI+TT S03980MWSU

pBAD+RBS+tetR+TT K091145MWSU

pBAD+RBS+luxR+TT K091179MWSU

RBS(B0030)+GFP E55002007 Parts Kit Plate 1 9J pSB1A2

RBS(B0034)+RFP MWSU

Mnt C00722009 Kit Plate 1 12L pSB2K3

LuxO-P K1310222009 Kit Plate 3 8K pSB1AC3

Parts needed to be built from scratch

prpBp Ecocyc

http://gcat.davidson.edu/GcatWiki/images/2/2f/PrpBp.JPG

prpB 2-methylisocitrate lyase in E.coli MG1655 [1]http://gcat.davidson.edu/GcatWiki/images/6/6c/PrpB.JPG

pLuxT [2] http://gcat.davidson.edu/GcatWiki/images/e/e3/LuxT_upstream.JPG

frame shift suppressor 1 CUGUCCUAA

frame shift suppressor 2 UUACUAGAC

References

Powerpoint File:Suppressorlogic3.ppt

Anderson et al. 2007. “Environmental signal integration by a modular AND gate” [3]

luxO luxT [4]

PprpB propionate [5]