Difference between revisions of "Synergy Machine Protocol"
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(Created page with 'Synergy Machine Protocol 1. Open Gen 5 software 2. Click on Existing protocol OR move to step 3 3. Click on Create New Protocol 4. Select Standard Protocol type 5. Click on Pro…') |
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Synergy Machine Protocol | Synergy Machine Protocol | ||
− | 1. Open Gen 5 software | + | #1. Open Gen 5 software |
− | 2. Click on Existing protocol OR move to step 3 | + | #2. Click on Existing protocol OR move to step 3 |
− | 3. Click on Create New Protocol | + | #3. Click on Create New Protocol |
− | 4. Select Standard Protocol type | + | #4. Select Standard Protocol type |
− | 5. Click on Procedure | + | #5. Click on Procedure |
− | 6. Under Shake, select Orbital Shake for 3 seconds | + | #6. Under Shake, select Orbital Shake for 3 seconds |
− | 7. Under Read, select Fluorescence from drop down menu | + | #7. Under Read, select Fluorescence from drop down menu |
− | a. Enter Emission and Excitation wavelengths | + | ##a. Enter Emission and Excitation wavelengths |
− | i. If you are reading more than one type of fluorescence, click the appropriate number of types above the wavelength values. | + | ###i. If you are reading more than one type of fluorescence, click the appropriate number of types above the wavelength values. |
− | b. For RFP, select 585 nm (excitation) and 615 nm (emission) for optimal reading (with 100 gain) | + | ##b. For RFP, select 585 nm (excitation) and 615 nm (emission) for optimal reading (with 100 gain) |
− | c. For GFP, select ___ (excitation) and ___ (emission) for optimal reading (with 100 gain) | + | ##c. For GFP, select ___ (excitation) and ___ (emission) for optimal reading (with 100 gain) |
− | 8. Under Read, select Absorbance from the drop down menu and enter 590 nm | + | #8. Under Read, select Absorbance from the drop down menu and enter 590 nm |
− | 9. Once those values have been submitted, drag tasks into proper order. First Shake, then read Fluorescence, then read Absorbance. | + | #9. Once those values have been submitted, drag tasks into proper order. First Shake, then read Fluorescence, then read Absorbance. |
− | a. If you are measuring GFP and RFP, measure GFP before RFP. | + | ##a. If you are measuring GFP and RFP, measure GFP before RFP. |
− | 10. Click OK, and from the banner menu, select File, New Task, then Read Now (selecting the procedure you created) | + | #10. Click OK, and from the banner menu, select File, New Task, then Read Now (selecting the procedure you created) |
− | 11. Place the well inside the machine, and Click OK when ready. | + | #11. Place the well inside the machine, and Click OK when ready. |
− | 12. After the machine has run, you can export the data to Excel using the Excel button on the banner of the window that pops up. | + | #12. After the machine has run, you can export the data to Excel using the Excel button on the banner of the window that pops up. |
Revision as of 21:06, 5 June 2012
Synergy Machine Protocol
- 1. Open Gen 5 software
- 2. Click on Existing protocol OR move to step 3
- 3. Click on Create New Protocol
- 4. Select Standard Protocol type
- 5. Click on Procedure
- 6. Under Shake, select Orbital Shake for 3 seconds
- 7. Under Read, select Fluorescence from drop down menu
- a. Enter Emission and Excitation wavelengths
- i. If you are reading more than one type of fluorescence, click the appropriate number of types above the wavelength values.
- b. For RFP, select 585 nm (excitation) and 615 nm (emission) for optimal reading (with 100 gain)
- c. For GFP, select ___ (excitation) and ___ (emission) for optimal reading (with 100 gain)
- a. Enter Emission and Excitation wavelengths
- 8. Under Read, select Absorbance from the drop down menu and enter 590 nm
- 9. Once those values have been submitted, drag tasks into proper order. First Shake, then read Fluorescence, then read Absorbance.
- a. If you are measuring GFP and RFP, measure GFP before RFP.
- 10. Click OK, and from the banner menu, select File, New Task, then Read Now (selecting the procedure you created)
- 11. Place the well inside the machine, and Click OK when ready.
- 12. After the machine has run, you can export the data to Excel using the Excel button on the banner of the window that pops up.