Difference between revisions of "Synergy Machine Protocol"
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Synergy Machine Protocol | Synergy Machine Protocol | ||
| − | # | + | # Open Gen 5 software |
| − | # | + | # Click on Existing protocol OR move to step 3 |
| − | # | + | # Click on Create New Protocol |
| − | # | + | # Select Standard Protocol type |
| − | # | + | # Click on Procedure |
| − | # | + | # Under Shake, select Orbital Shake for 3 seconds |
| − | # | + | # Under Read, select Fluorescence from drop down menu |
| − | ## | + | ## Enter Emission and Excitation wavelengths |
| − | ### | + | ### If you are reading more than one type of fluorescence, click the appropriate number of types above the wavelength values. |
| − | ## | + | ## For RFP, select 585 nm (excitation) and 615 nm (emission) for optimal reading (with 100 gain) |
| − | ## | + | ## For GFP, select ___ (excitation) and ___ (emission) for optimal reading (with 100 gain) |
| − | # | + | # Under Read, select Absorbance from the drop down menu and enter 590 nm |
| − | # | + | # Once those values have been submitted, drag tasks into proper order. First Shake, then read Fluorescence, then read Absorbance. |
| − | ## | + | ## If you are measuring GFP and RFP, measure GFP before RFP. |
| − | # | + | # Click OK, and from the banner menu, select File, New Task, then Read Now (selecting the procedure you created) |
| − | # | + | # Place the well inside the machine, and Click OK when ready. |
| − | # | + | # After the machine has run, you can export the data to Excel using the Excel button on the banner of the window that pops up. |
Revision as of 21:07, 5 June 2012
Synergy Machine Protocol
- Open Gen 5 software
- Click on Existing protocol OR move to step 3
- Click on Create New Protocol
- Select Standard Protocol type
- Click on Procedure
- Under Shake, select Orbital Shake for 3 seconds
- Under Read, select Fluorescence from drop down menu
- Enter Emission and Excitation wavelengths
- If you are reading more than one type of fluorescence, click the appropriate number of types above the wavelength values.
- For RFP, select 585 nm (excitation) and 615 nm (emission) for optimal reading (with 100 gain)
- For GFP, select ___ (excitation) and ___ (emission) for optimal reading (with 100 gain)
- Enter Emission and Excitation wavelengths
- Under Read, select Absorbance from the drop down menu and enter 590 nm
- Once those values have been submitted, drag tasks into proper order. First Shake, then read Fluorescence, then read Absorbance.
- If you are measuring GFP and RFP, measure GFP before RFP.
- Click OK, and from the banner menu, select File, New Task, then Read Now (selecting the procedure you created)
- Place the well inside the machine, and Click OK when ready.
- After the machine has run, you can export the data to Excel using the Excel button on the banner of the window that pops up.
