TAS2R38 PCR amplification

1. When the DNA extraction cools (Isolate_genomic_DNA_from_a_single_hair_follicle), vortex the tubes for 30 seconds and then set up a new 500 µL microfuge tube by adding the following:

Reagent	Volume
extracted DNA	10.5 µL
2X Green GoTaq Mix	12.5 µL
Forward Primer	1.0 µL
Reverse Primer	1.0 µL
Final Volume	25.0 µL

2. Run the thermocycler program called TAS2R38 with the heated lid enabled. PCR conditions:

1. 5 minutes at 95˚C.
2. 1 minute at 95˚C.
3. 1 minute at 65˚C.
4. 1 minute at 72˚C.
5. repeat steps 2 - 5 29 more times.
6. hold at room temperature.
7. END

3. When the PCR is completed (~2 hours), store DNA frozen until you are ready to clean up the DNA in order to have your two alleles sequenced.

4. Before you can send your DNA off for sequencing, you will need to clean up your PCR products

Reference: