Difference between revisions of "TBLASTn and Protein Sequence Analysis"

From GcatWiki
Jump to: navigation, search
(When Nucleotides Don't Work)
(When Nucleotides Don't Work)
Line 9: Line 9:
 
If, for any of these reasons, you aren't getting any hits, it might be time to make sure you're doing it right:
 
If, for any of these reasons, you aren't getting any hits, it might be time to make sure you're doing it right:
  
First -
+
::'''''First''''' -
  
 
== Beyond blastn ==
 
== Beyond blastn ==
  
 
== Why Amino Acids? ==
 
== Why Amino Acids? ==

Revision as of 18:36, 22 February 2011

When Nucleotides Don't Work

Occasionally BLASTing genes with just a nucleotide sequence doesn't work. This can be due to a number of reasons:

  • Lack of conservation between species
  • Incomplete genome database
  • Rearrangement of introns/exons

If, for any of these reasons, you aren't getting any hits, it might be time to make sure you're doing it right:

First -

Beyond blastn

Why Amino Acids?