Difference between revisions of "Template Preparation for RT-qPCR"
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Bgrieshaber (talk | contribs) (Created page with "'''Template Preparation''' 1. Place 1mL of grown bacteria into a 1.5mL tube. 2. Heat the tube at 95C for 5min in a water bath. (If tubes pop open frequently try reducing the...") |
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1. Place 1mL of grown bacteria into a 1.5mL tube. | 1. Place 1mL of grown bacteria into a 1.5mL tube. | ||
| − | 2. Heat the tube at 95C for | + | 2. Heat the tube at 95C for 10min in a water bath. |
| − | 3. Immediately after heating, place the tubes in a -20C cooler/freezer for | + | 3. Immediately after heating, place the tubes in a -20C cooler/freezer for 40-60min or until frozen. |
4. Take out and allow to thaw for dilutions. | 4. Take out and allow to thaw for dilutions. | ||
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'''Dilutions of Prepared Templates''' | '''Dilutions of Prepared Templates''' | ||
| − | After prepared templates have thawed perform serial dilutions of | + | After prepared templates have thawed perform serial dilutions of 10^2, 10^2.5, 10^3, 10^3.5, 10^4 making sure to vortex before each dilution. |
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Latest revision as of 16:33, 30 July 2013
Template Preparation
1. Place 1mL of grown bacteria into a 1.5mL tube.
2. Heat the tube at 95C for 10min in a water bath.
3. Immediately after heating, place the tubes in a -20C cooler/freezer for 40-60min or until frozen.
4. Take out and allow to thaw for dilutions.
Dilutions of Prepared Templates
After prepared templates have thawed perform serial dilutions of 10^2, 10^2.5, 10^3, 10^3.5, 10^4 making sure to vortex before each dilution.
