Difference between revisions of "Test pLuxR/cI"
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luxR/CI hybrid promoter is constitutively off. Ligate with GFP on the 3’ end. If luxR is present first then add AI-1, the bacteria should glow green. Then add CI, the bacteria should not glow. | luxR/CI hybrid promoter is constitutively off. Ligate with GFP on the 3’ end. If luxR is present first then add AI-1, the bacteria should glow green. Then add CI, the bacteria should not glow. | ||
| − | Some promoter→LuxR+t+t+pLuxR/cI→GFP does not | + | Some promoter→LuxR+t+t+pLuxR/cI→GFP does not glow |
Latest revision as of 20:17, 15 July 2008
luxR/CI hybrid promoter is constitutively off. Ligate with GFP on the 3’ end. If luxR is present first then add AI-1, the bacteria should glow green. Then add CI, the bacteria should not glow.
Some promoter→LuxR+t+t+pLuxR/cI→GFP does not glow
add AI-1
Some promoter→LuxR+t+t+pLuxR/cI→GFP glows
add AI-1
Some promoter→LuxR+cI+t+t+pLuxR/cI→GFP does not glow
| LuxR | cI | AI-1 + GFP | AI-1 - GFP |
|---|---|---|---|
| 0 | 0 | 0 | 0 |
| 1 | 0 | 1 | 0 |
| 0 | 1 | 0 | 0 |
| 1 | 1 | 0 | 0 |
