Difference between revisions of "Washing Beads"
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(Created page with "'''Protocol for washing the plating beads used for bacterial distribution''' '''Step 1''' Place used beads in a large beaker (depending on amount of beads) and soak for 30 m...") |
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'''Step 1''' | '''Step 1''' | ||
| − | Place used beads in a large beaker (depending on amount of beads) and soak for 30 minutes in dishwashing soap and hot water. | + | Place used beads in a large beaker (depending on amount of beads) and soak for 30 minutes in dishwashing soap and hot water, stirring occasionally. |
'''Step 2''' | '''Step 2''' | ||
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'''Step 3''' | '''Step 3''' | ||
| − | Dry beaker, then take beads from sieve and place in the beaker with 95% EtOH. Soak 30 minutes. | + | Dry beaker, then take beads from sieve and place in the beaker with 95% EtOH. Soak 30 minutes, stirring occasionally. |
'''Step 4''' | '''Step 4''' | ||
Latest revision as of 14:27, 17 June 2014
Protocol for washing the plating beads used for bacterial distribution
Step 1
Place used beads in a large beaker (depending on amount of beads) and soak for 30 minutes in dishwashing soap and hot water, stirring occasionally.
Step 2
Rinse beads after 30 minutes in a sieve (or strainer) with cold water.
Step 3
Dry beaker, then take beads from sieve and place in the beaker with 95% EtOH. Soak 30 minutes, stirring occasionally.
Step 4
Place in sieve for 5 minutes.
Step 5
Let dry by placing in a warm autoclave for 1 hour (WARNING: DO NOT RUN AUTOCLAVE)
Step 6
Place dry beads in bottles or smaller beakers with a funnel, then autoclave with the caps loosened (if using beakers, just place aluminum foil over the top).
