Difference between revisions of "Wet Lab Pages"

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m (Registry parts to clone)
(Registry parts to clone)
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!width="225"|Description
 
!width="225"|Description
 
!width="225"|Student Responsible (DC or MW)
 
!width="225"|Student Responsible (DC or MW)
!width="30"|Part # and Vector
+
!width="30"|Location and Vector
 
!width="30"|Resistance
 
!width="30"|Resistance
 
!width="30"|Results from 5/22/08
 
!width="30"|Results from 5/22/08
 
|-
 
|-
|BBa_F2621|| AHL receiver with lux pR and codes for LuxR || Karlesha Roland (DC) || 1001 12B PSB1A2 || amp || growth MW
+
|BBa_F2621|| AHL receiver with lux pR and codes for LuxR || Karlesha Roland (DC) || 1001 12B pSB1A2 || amp || growth MW
 
|-
 
|-
 
|BBa_I15030 || Lux-sender(Autoinducing)Codes for LuxI and LuxR || Kelly Davis (DC)  will this send to part BBa_I13263 || 1015 8F pSB3K3 || kan || growth MW
 
|BBa_I15030 || Lux-sender(Autoinducing)Codes for LuxI and LuxR || Kelly Davis (DC)  will this send to part BBa_I13263 || 1015 8F pSB3K3 || kan || growth MW
 
|-
 
|-
|BBa_I13263 || Lux Receiver (HSL & R0063 driven)produces YFP || Pallavi Penumetcha (DC)|| 1002 8E SB1A2 || amp || growth MW & DC
+
|BBa_I13263 || Lux Receiver (HSL & R0063 driven)produces YFP || Pallavi Penumetcha (DC)|| 1002 8E pSB1A2 || amp || growth MW & DC
 
|-
 
|-
|BBa_f2622 || 3OC6HSL receiver that outputs PoPS || James Barron (DC) || 1001 12E SB1A2 || amp || no growth
+
|BBa_f2622 || 3OC6HSL receiver that outputs PoPS || James Barron (DC) || 1001 12E pSB1A2 || amp || no growth
 
|-
 
|-
|BBa_F1610 || Device that receives PoPS and outputs LuxI || Malcolm Campbell (DC) || 1016 10D SB1AK3 || amp & kan || growth MW
+
|BBa_F1610 || Device that receives PoPS and outputs LuxI || Malcolm Campbell (DC) || 1016 10D pSB1AK3 || amp & kan || growth MW
 
|-
 
|-
|BBa_I0426 || Las-reciver(EYFP) || Kristi Muscalino (DC) || 1008 5B SB1A2 || amp || growth MW & DC
+
|BBa_I0426 || Las-reciver(EYFP) || Kristi Muscalino (DC) || 1008 5B pSB1A2 || amp || growth MW & DC
 
|-
 
|-
|BBa_I0407 || LasI test || Erin Feeney (DC) || 1008 4F SB1A2 || amp || growth MW & DC
+
|BBa_I0407 || LasI test || Erin Feeney (DC) || 1008 4F pSB1A2 || amp || growth MW & DC
 
|-
 
|-
|BBa_I0466|| RhlR Protein Generator without LVA || Laurie Heyer (DC) || 1001 5C SB1A2 || amp || growth MW
+
|BBa_I0466|| RhlR Protein Generator without LVA || Laurie Heyer (DC) || 1001 5C pSB1A2 || amp || growth MW
 
|-
 
|-
 
|}
 
|}
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!width="225"|Description
 
!width="225"|Description
 
!width="225"|Student Responsible (DC or MW)
 
!width="225"|Student Responsible (DC or MW)
!width="30"|Part # and Vector
+
!width="30"|Location and Vector
 
!width="30"|Resistance
 
!width="30"|Resistance
 
!width="30"|Results from 5/22/08
 
!width="30"|Results from 5/22/08
 
|-
 
|-
|BBa_C0061 || LuxI gene with LVA || Madeline Parra (DC) || 1000 3E SB1A2 || amp || growth MW
+
|BBa_C0061 || LuxI gene with LVA || Madeline Parra (DC) || 1000 3E pSB1A2 || amp || growth MW
 
|-
 
|-
|BBa_C0161 || LuxI gene without LVA || Xiao Zhu (MW) || 1001 8B SB1A2 || amp ||
+
|BBa_C0161 || LuxI gene without LVA || Xiao Zhu (MW) || 1001 8B pSB1A2 || amp ||
 
|-
 
|-
|BBa_C0062 || LuxR gene without LVA || John Igo (MW) || 1000 3F SB1A2 || amp ||
+
|BBa_C0062 || LuxR gene without LVA || John Igo (MW) || 1000 3F pSB1A2 || amp ||
 
|-
 
|-
|BBa_R0063 || lux pL|| Aaron Lewis (MW) || 1000 4H SB1A2 || amp ||  
+
|BBa_R0063 || lux pL|| Aaron Lewis (MW) || 1000 4H pSB1A2 || amp ||  
 
|-
 
|-
|BBa_R0062|| lux pR|| Andrew Gordon(MW) || 1000 4G SB1A2 || amp ||
+
|BBa_R0062|| lux pR|| Andrew Gordon(MW) || 1000 4G pSB1A2 || amp ||
 
|-
 
|-
|BBa_C0070||  rhlI gene  ||  Jeff Poet (MW) || 1013 3E SB2K3 || kan ||  
+
|BBa_C0070||  rhlI gene  ||  Jeff Poet (MW) || 1013 3E pSB2K3 || kan ||  
 
|-
 
|-
|BBa_C0071|| rhlR gene with LVA|| Max Win (DC) || 1013 3F SB2K3 || kan || no growth
+
|BBa_C0071|| rhlR gene with LVA|| Max Win (DC) || 1013 3F pSB2K3 || kan || no growth
 
|-
 
|-
|BBa_C0171|| rhlR gene without LVA || MWSU || 1001 6F SB1A2 || amp ||  
+
|BBa_C0171|| rhlR gene without LVA || MWSU || 1001 6F pSB1A2 || amp ||  
 
|-
 
|-
|BBa_R0079 || las pR || Todd Eckdahl (MW) || 1001 10F SB1A2 || amp ||  
+
|BBa_R0079 || las pR || Todd Eckdahl (MW) || 1001 10F pSB1A2 || amp ||  
 
|-
 
|-
|BBa_C0079 || lasR gene || Robert Cool (MW) || 1013 4F SB2K3 || kan ||  
+
|BBa_C0079 || lasR gene || Robert Cool (MW) || 1013 4F pSB2K3 || kan ||  
 
|-
 
|-
|BBa_C0078 || LasI gene || Alicia Allen (MW) || 1013 4E SB2K3 || kan ||  
+
|BBa_C0078 || LasI gene || Alicia Allen (MW) || 1013 4E pSB2K3 || kan ||  
 
|-
 
|-
|BBa_J07019 || fecA promoter with Fur box || Not in Registry
+
|BBa_J07019 || fecA promoter with Fur box || Not in Registry|| || ||
 
|-
 
|-
|BBa_#### || Description || Sven(MW)
+
|BBa_#### || Description || Sven(MW) || || ||
 
|-
 
|-
|BBa_#### || Description || Sven(MW)
+
|BBa_#### || Description || Sven(MW) || || ||
 
|-
 
|-
|BBa_#### || Description || Sven(MW)
+
|BBa_#### || Description || Sven(MW) || || ||
 +
|}
 +
 
 +
'''Fluorescent Protein Gradient Testers''':
 +
 
 +
{| border="1" cellpadding="2"
 +
!width="50"|Part
 +
!width="225"|Description
 +
!width="225"|Student Responsible (DC or MW)
 +
!width="30"|Location and Vector
 +
!width="30"|Resistance
 +
!width="30"|Results
 +
|-
 +
|BBa_#### || Description || Surfer dude (DC) || || ||
 +
|-
 +
|BBa_#### || Description || Surfer dude (DC) || || ||
 +
|-
 +
|BBa_#### || Description || Surfer dude (DC) || || ||
 +
|-
 +
|BBa_#### || Description || Surfer dude (DC) || || ||
 
|}
 
|}

Revision as of 13:26, 23 May 2008

This is the space for MWSU and DC wet lab students to create content. Our part numbers will be in this range only: BBa_K091000 to BBa_K091999.

  1. Davidson Wet Lab Protocols
  2. MWSU Wet Lab Protocols
  3. Plasmid Compatability
  4. Davidson -80 Stocks
  5. FAQs for Wikis



Registry parts to clone

Composite Parts:

Part Description Student Responsible (DC or MW) Location and Vector Resistance Results from 5/22/08
BBa_F2621 AHL receiver with lux pR and codes for LuxR Karlesha Roland (DC) 1001 12B pSB1A2 amp growth MW
BBa_I15030 Lux-sender(Autoinducing)Codes for LuxI and LuxR Kelly Davis (DC) will this send to part BBa_I13263 1015 8F pSB3K3 kan growth MW
BBa_I13263 Lux Receiver (HSL & R0063 driven)produces YFP Pallavi Penumetcha (DC) 1002 8E pSB1A2 amp growth MW & DC
BBa_f2622 3OC6HSL receiver that outputs PoPS James Barron (DC) 1001 12E pSB1A2 amp no growth
BBa_F1610 Device that receives PoPS and outputs LuxI Malcolm Campbell (DC) 1016 10D pSB1AK3 amp & kan growth MW
BBa_I0426 Las-reciver(EYFP) Kristi Muscalino (DC) 1008 5B pSB1A2 amp growth MW & DC
BBa_I0407 LasI test Erin Feeney (DC) 1008 4F pSB1A2 amp growth MW & DC
BBa_I0466 RhlR Protein Generator without LVA Laurie Heyer (DC) 1001 5C pSB1A2 amp growth MW

Basic Parts:

Part Description Student Responsible (DC or MW) Location and Vector Resistance Results from 5/22/08
BBa_C0061 LuxI gene with LVA Madeline Parra (DC) 1000 3E pSB1A2 amp growth MW
BBa_C0161 LuxI gene without LVA Xiao Zhu (MW) 1001 8B pSB1A2 amp
BBa_C0062 LuxR gene without LVA John Igo (MW) 1000 3F pSB1A2 amp
BBa_R0063 lux pL Aaron Lewis (MW) 1000 4H pSB1A2 amp
BBa_R0062 lux pR Andrew Gordon(MW) 1000 4G pSB1A2 amp
BBa_C0070 rhlI gene Jeff Poet (MW) 1013 3E pSB2K3 kan
BBa_C0071 rhlR gene with LVA Max Win (DC) 1013 3F pSB2K3 kan no growth
BBa_C0171 rhlR gene without LVA MWSU 1001 6F pSB1A2 amp
BBa_R0079 las pR Todd Eckdahl (MW) 1001 10F pSB1A2 amp
BBa_C0079 lasR gene Robert Cool (MW) 1013 4F pSB2K3 kan
BBa_C0078 LasI gene Alicia Allen (MW) 1013 4E pSB2K3 kan
BBa_J07019 fecA promoter with Fur box Not in Registry
BBa_#### Description Sven(MW)
BBa_#### Description Sven(MW)
BBa_#### Description Sven(MW)

Fluorescent Protein Gradient Testers:

Part Description Student Responsible (DC or MW) Location and Vector Resistance Results
BBa_#### Description Surfer dude (DC)
BBa_#### Description Surfer dude (DC)
BBa_#### Description Surfer dude (DC)
BBa_#### Description Surfer dude (DC)